Endothelial Progenitors: A Consensus Statement on Nomenclature.
Reinhold J. Medina,Chad L. Barber,Florence Sabatier,Françoise Dignat-George,Juan M. Melero-Martin,Juan M. Melero-Martin,Kiarash Khosrotehrani,Osamu Ohneda,Anna M. Randi,Jerry Kok Yen Chan,Teruhide Yamaguchi,Victor W.M. van Hinsbergh,Mervin C. Yoder,Alan W. Stitt +13 more
TLDR
This review seeks to discourage the indiscriminate use of “EPCs,” and instead proposes precise terminology based on defining cellular phenotype and function, which should become more precise in the light of strong scientific evidence.Abstract:
Endothelial progenitor cell (EPC) nomenclature remains ambiguous and there is a general lack of concordance in the stem cell field with many distinct cell subtypes continually grouped under the term “EPC.” It would be highly advantageous to agree on standards to confirm an endothelial progenitor phenotype and this should include detailed immunophenotyping, potency assays, and clear separation from hematopoietic angiogenic cells which are not endothelial progenitors. In this review, we seek to discourage the indiscriminate use of “EPCs,” and instead propose precise terminology based on defining cellular phenotype and function. Endothelial colony forming cells and myeloid angiogenic cells are examples of two distinct and well-defined cell types that have been considered EPCs because they both promote vascular repair, albeit by completely different mechanisms of action. It is acknowledged that scientific nomenclature should be a dynamic process driven by technological and conceptual advances; ergo the ongoing “EPC” nomenclature ought not to be permanent and should become more precise in the light of strong scientific evidence. This is especially important as these cells become recognized for their role in vascular repair in health and disease and, in some cases, progress toward use in cell therapy.read more
Citations
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Consensus guidelines for the use and interpretation of angiogenesis assays
Patrycja Nowak-Sliwinska,Kari Alitalo,Elizabeth Allen,Andrey Anisimov,Alfred C. Aplin,Robert Auerbach,Hellmut G. Augustin,Hellmut G. Augustin,David O. Bates,Judy R. van Beijnum,R. Hugh F. Bender,Gabriele Bergers,Gabriele Bergers,Andreas Bikfalvi,Joyce Bischoff,Barbara C. Böck,Barbara C. Böck,Peter C. Brooks,Federico Bussolino,Bertan Cakir,Peter Carmeliet,Daniel Castranova,Anca Maria Cimpean,Ondine Cleaver,George Coukos,George E. Davis,Michele De Palma,Anna Dimberg,Ruud P.M. Dings,Valentin Djonov,Andrew C. Dudley,Neil Dufton,Sarah-Maria Fendt,Napoleone Ferrara,Marcus Fruttiger,Dai Fukumura,Bart Ghesquière,Bart Ghesquière,Yan Gong,Robert J. Griffin,Adrian L. Harris,Christopher C.W. Hughes,Nan W. Hultgren,M. Luisa Iruela-Arispe,Melita Irving,Rakesh K. Jain,Raghu Kalluri,Joanna Kalucka,Robert S. Kerbel,Jan Kitajewski,Ingeborg Klaassen,Hynda K. Kleinmann,Pieter Koolwijk,Elisabeth Kuczynski,Brenda R. Kwak,Koen Marien,Juan M. Melero-Martin,Lance L. Munn,Roberto F. Nicosia,Agnès Noël,Jussi Nurro,Anna-Karin Olsson,Tatiana V. Petrova,Kristian Pietras,Roberto Pili,Jeffrey W. Pollard,Mark J. Post,Paul H.A. Quax,Gabriel A. Rabinovich,Marius Raica,Anna M. Randi,Domenico Ribatti,Curzio Rüegg,Reinier O. Schlingemann,Reinier O. Schlingemann,Stefan Schulte-Merker,Lois E.H. Smith,Jonathan W. Song,Steven A. Stacker,Jimmy Stalin,Amber N. Stratman,Maureen Van de Velde,Victor W.M. van Hinsbergh,Peter B. Vermeulen,Johannes Waltenberger,Brant M. Weinstein,Hong Xin,Bahar Yetkin-Arik,Seppo Ylä-Herttuala,Mervin C. Yoder,Arjan W. Griffioen +90 more
TL;DR: In vivo, ex vivo, and in vitro bioassays that are available for the evaluation of angiogenesis are described and critical aspects that are relevant for their execution and proper interpretation are highlighted.
Journal ArticleDOI
Tissue Engineering at the Blood-Contacting Surface: A Review of Challenges and Strategies in Vascular Graft Development.
TL;DR: The current state of TEVGs is reviewed and several strategies to modify blood‐contacting surfaces to resist thrombosis and control cellular recruitment are reviewed, including coatings of biomimetic peptides and heparin.
Journal ArticleDOI
Current understanding of the molecular and cellular pathology of diabetic retinopathy.
TL;DR: In this article, a review of retinal pathophysiology during diabetes mellitus has been presented, which has uncovered potential new therapeutic avenues to treat this debilitating disease, which leads to profound vascular abnormalities, loss of the blood-retinal barrier and impaired neuronal function.
Journal ArticleDOI
Endothelial function in cardiovascular medicine: a consensus paper of the European Society of Cardiology Working Groups on Atherosclerosis and Vascular Biology, Aorta and Peripheral Vascular Diseases, Coronary Pathophysiology and Microcirculation, and Thrombosis.
Yvonne Alexander,Elena Osto,Elena Osto,Arno Schmidt-Trucksäss,Michael Shechter,Michael Shechter,Danijela Trifunovic,Dirk J. Duncker,Victor Aboyans,Magnus Bäck,Magnus Bäck,Lina Badimon,Francesco Cosentino,Marco De Carlo,Maria Dorobantu,David G. Harrison,Tomasz J. Guzik,Tomasz J. Guzik,Imo E. Hoefer,Paul Morris,Giuseppe Danilo Norata,Rosa Suades,Stefano Taddei,Gemma Vilahur,Johannes Waltenberger,Christian Weber,Fiona L. Wilkinson,Marie-Luce Bochaton-Piallat,Paul C. Evans +28 more
TL;DR: It is proposed that a consensus methodology for FMD is universally adopted to minimize technical variation between studies, and that reference FMD values are established for different populations of healthy individuals and patient groups.
Journal ArticleDOI
YAP and TAZ limit cytoskeletal and focal adhesion maturation to enable persistent cell motility.
Devon E. Mason,Devon E. Mason,Joseph M. Collins,James H. Dawahare,Trung Dung Nguyen,Trung Dung Nguyen,Yang Lin,Sherry L. Voytik-Harbin,Pinar Zorlutuna,Mervin C. Yoder,Joel D. Boerckel,Joel D. Boerckel +11 more
TL;DR: It is found that global inhibition of transcription or translation does not impair initial cell polarization or migration initiation, but causes eventual migratory arrest through excessive cytoskeletal tension and over-maturation of focal adhesion reinforcement.
References
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Isolation of putative progenitor endothelial cells for angiogenesis.
Takayuki Asahara,Toyoaki Murohara,Alison Sullivan,Marcy Silver,Rien van der Zee,Tong Li,Bernhard Witzenbichler,Gina C. Schatteman,Jeffrey M. Isner +8 more
TL;DR: It is suggested that EC progenitors may be useful for augmenting collateral vessel growth to ischemic tissues (therapeutic angiogenesis) and for delivering anti- or pro-angiogenic agents, respectively, to sites of pathologic or utilitarianAngiogenesis.
Journal ArticleDOI
Expression of VEGFR-2 and AC133 by circulating human CD34+ cells identifies a population of functional endothelial precursors
Mario Peichev,Afzal J. Naiyer,Daniel S. Pereira,Zhenping Zhu,William J. Lane,Mathew R. Williams,Mehmet C. Oz,Daniel J. Hicklin,Larry Witte,Malcolm A.S. Moore,Shahin Rafii +10 more
TL;DR: In an in vivo human model, it is found that the neo-intima formed on the surface of left ventricular assist devices is colonized with AC133(+)VEGFR-2(+) cells, suggesting a phenotypically and functionally distinct population of circulating endothelial cells that may play a role in neo-angiogenesis.
Journal ArticleDOI
Circulating endothelial progenitor cells and cardiovascular outcomes.
Nikos Werner,Sonja Kosiol,Tobias Schiegl,Patrick Ahlers,Katrin Walenta,Andreas Link,Michael Böhm,Georg Nickenig +7 more
TL;DR: The association between baseline levels of endothelial progenitor cells and death from cardiovascular causes, the occurrence of a first major cardiovascular event, revascularization, hospitalization, anddeath from all causes were evaluated.
Journal ArticleDOI
Origins of circulating endothelial cells and endothelial outgrowth from blood
TL;DR: Analysis of blood samples from bone marrow transplant recipients who had received gender-mismatched transplants 5-20 months earlier showed that most CEC in fresh blood had recipient genotype, and data indicate that outgrowth of endothelial cells from cultures of blood is mostly derived from transplantable marrow-derived cells.
Journal ArticleDOI
Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood.
David A. Ingram,Laura E. Mead,Hiromi Tanaka,Virginia M. Meade,Amy Fenoglio,Kelly E. Mortell,Karen E. Pollok,Michael J. Ferkowicz,David Gilley,Mervin C. Yoder +9 more
TL;DR: These studies describe a clonogenic method to define a hierarchy of EPCs based on their proliferative potential, and they identify a unique population of high proliferation potential-endothelial colony-forming cells (HPP-ECFCs) in human umbilical cord blood.