High-resolution bacterial 16S rRNA gene profile meta-analysis and biofilm status reveal common colorectal cancer consortia.
Julia L. Drewes,James R. White,Christine M. Dejea,Payam Fathi,Thevambiga Iyadorai,Jamuna Vadivelu,April Camilla Roslani,Elizabeth C. Wick,Emmanuel F. Mongodin,Mun Fai Loke,Kumar Thulasi,Han Ming Gan,Khean-Lee Goh,Hoong Yin Chong,Sandip Kumar,Jane W. Wanyiri,Cynthia L. Sears +16 more
- Vol. 3, Iss: 1, pp 34-34
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TLDR
Researchers in the USA and Malaysia found that bowel cancers were enriched in invasive bacterial biofilms as well as several specific gut and oral species, including one - Fusobacterium nucleatum - known to promote tumorigenesis in mouse models.Abstract:
Colorectal cancer (CRC) remains the third most common cancer worldwide, with a growing incidence among young adults. Multiple studies have presented associations between the gut microbiome and CRC, suggesting a link with cancer risk. Although CRC microbiome studies continue to profile larger patient cohorts with increasingly economical and rapid DNA sequencing platforms, few common associations with CRC have been identified, in part due to limitations in taxonomic resolution and differences in analysis methodologies. Complementing these taxonomic studies is the newly recognized phenomenon that bacterial organization into biofilm structures in the mucus layer of the gut is a consistent feature of right-sided (proximal), but not left-sided (distal) colorectal cancer. In the present study, we performed 16S rRNA gene amplicon sequencing and biofilm quantification in a new cohort of patients from Malaysia, followed by a meta-analysis of eleven additional publicly available data sets on stool and tissue-based CRC microbiota using Resphera Insight, a high-resolution analytical tool for species-level characterization. Results from the Malaysian cohort and the expanded meta-analysis confirm that CRC tissues are enriched for invasive biofilms (particularly on right-sided tumors), a symbiont with capacity for tumorigenesis (Bacteroides fragilis), and oral pathogens including Fusobacterium nucleatum, Parvimonas micra, and Peptostreptococcus stomatis. Considered in aggregate, species from the Human Oral Microbiome Database are highly enriched in CRC. Although no detected microbial feature was universally present, their substantial overlap and combined prevalence supports a role for the gut microbiota in a significant percentage (>80%) of CRC cases.read more
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Tumor Microbiome Diversity and Composition Influence Pancreatic Cancer Outcomes
Erick Riquelme,Erick Riquelme,Yu Zhang,Liangliang Zhang,Maria Fernanda Montiel,Michelle Zoltan,Wenli Dong,Pompeyo R. Quesada,Ismet Sahin,Vidhi Chandra,Anthony San Lucas,Paul Scheet,Hanwen Xu,Samir M. Hanash,Lei Feng,Jared K. Burks,Kim Anh Do,Christine B. Peterson,Deborah Nejman,Ching Wei D. Tzeng,Michael P. Kim,Cynthia L. Sears,Nadim J. Ajami,Joseph F. Petrosino,Laura D. Wood,Anirban Maitra,Ravid Straussman,Matthew H.G. Katz,James R. White,Robert R. Jenq,Jennifer A. Wargo,Florencia McAllister +31 more
TL;DR: This study demonstrates that PDAC microbiome composition, which cross-talks to the gut microbiome, influences the host immune response and natural history of the disease.
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TL;DR: The role of microorganisms in colorectal carcinogenesis, and the potential clinical translation of the gut microbiota as a biomarker for CRC diagnosis and prognosis are described, and as an approach for disease prevention and to improve therapy are described.
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Metagenomic analysis of colorectal cancer datasets identifies cross-cohort microbial diagnostic signatures and a link with choline degradation
Andrew Maltez Thomas,Andrew Maltez Thomas,Paolo Manghi,Francesco Asnicar,Edoardo Pasolli,Federica Armanini,Moreno Zolfo,Francesco Beghini,Serena Manara,Nicolai Karcher,Chiara Pozzi,Sara Gandini,Davide Serrano,Sonia Tarallo,Antonio Francavilla,Gaetano Gallo,Mario Trompetto,Giulio Ferrero,Sayaka Mizutani,Sayaka Mizutani,Hirotsugu Shiroma,Satoshi Shiba,Tatsuhiro Shibata,Shinichi Yachida,Takuji Yamada,Takuji Yamada,Jakob Wirbel,Petra Schrotz-King,Cornelia M. Ulrich,Hermann Brenner,Manimozhiyan Arumugam,Manimozhiyan Arumugam,Peer Bork,Georg Zeller,Francesca Cordero,Emmanuel Dias-Neto,João C. Setubal,João C. Setubal,Adrian Tett,Barbara Pardini,Maria Rescigno,Levi Waldron,Alessio Naccarati,Nicola Segata +43 more
TL;DR: The combined analysis of heterogeneous CRC cohorts identified reproducible microbiome biomarkers and accurate disease-predictive models that can form the basis for clinical prognostic tests and hypothesis-driven mechanistic studies.
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The Intestinal Microbiota in Colorectal Cancer
TL;DR: Large metagenomic studies in human CRC associated microbiome signatures with the colorectal adenoma-carcinoma sequence suggest a fundamental role of the intestinal microbiota in the evolution of gastrointestinal malignancy.
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Host-microbiota maladaptation in colorectal cancer.
TL;DR: The host-microbiota interactions in CRC are discussed, with particular focus on mechanisms that are linked to intestinal barrier disruption, genotoxicity and deleterious inflammation.
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