Integrative Genomics Identifies the Corepressor SMRT as a Gatekeeper of Adipogenesis through the Transcription Factors C/EBPβ and KAISO
Sunil K. Raghav,Sebastian M. Waszak,Irina Krier,Carine Gubelmann,Alina Isakova,Tarjei S. Mikkelsen,Tarjei S. Mikkelsen,Bart Deplancke +7 more
TLDR
Genome-wide DNA-binding profiling revealed that this corepressor SMRT is predominantly located in active chromatin regions and that most distal SMRT binding events are lost after differentiation induction, revealing a role for SMRT in masking enhancers from proadipogenic factors in preadipocytes.About:
This article is published in Molecular Cell.The article was published on 2012-05-11 and is currently open access. It has received 87 citations till now. The article focuses on the topics: Corepressor & Adipogenesis.read more
Citations
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The Genetics of Transcription Factor DNA Binding Variation
TL;DR: The findings that led to this important paradigm shift are summarized and proposed mechanisms for local, proximal, or distal genetic variation-driven variable TF-DNA binding are reviewed.
Journal ArticleDOI
Mapping genome-wide transcription-factor binding sites using DAP-seq.
Anna Bartlett,Ronan C. O'Malley,Ronan C. O'Malley,Shao-shan Carol Huang,Mary Galli,Mary Galli,Joseph R. Nery,Andrea Gallavotti,Joseph R. Ecker +8 more
TL;DR: DAP-seq is developed, a transcription factor (TF)-binding site (TFBS) discovery assay that couples affinity-purified TFs with next-generation sequencing of a genomic DNA library, enabling low-cost, high-throughput generation of cistrome and epicistrome maps for any organism.
Journal ArticleDOI
Emerging roles of the corepressors NCoR1 and SMRT in homeostasis
TL;DR: The results from recent in vivo studies that reveal the wide-ranging roles of NCoR1 and SMRT in developmental as well as homeostatic processes, including metabolism, inflammation, and circadian rhythms are summarized.
Journal ArticleDOI
Gain Fat???Lose Metastasis: Converting Invasive Breast Cancer Cells into Adipocytes Inhibits Cancer Metastasis
Dana Ishay-Ronen,Maren Diepenbruck,Ravi Kiran Reddy Kalathur,Nami Sugiyama,Stefanie Tiede,Robert Ivanek,Glenn R. Bantug,Marco Francesco Morini,Junrong Wang,Christoph Hess,Gerhard Christofori +10 more
TL;DR: It is demonstrated that cancer cell plasticity can be exploited therapeutically by forcing the trans-differentiation of EMT-derived breast cancer cells into post-mitotic and functional adipocytes leading to the repression of primary tumor invasion and metastasis formation.
Journal ArticleDOI
Dynamic Rewiring of Promoter-Anchored Chromatin Loops during Adipocyte Differentiation
Rasmus Siersbæk,Jesper Grud Skat Madsen,Biola M. Javierre,Ronni Nielsen,Emilie Kristine Bagge,Jonathan Cairns,Steven W. Wingett,Sofie Traynor,Mikhail Spivakov,Peter Fraser,Peter Fraser,Susanne Mandrup +11 more
TL;DR: Intriguingly, formation of loops connecting activated enhancers and promoters is also associated with extensive recruitment of corepressors such as NCoR and HDACs, indicating that this class of coregulators may play a previously unrecognized role during enhancer activation.
References
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