Anna Bartlett

TL;DR: DNA affinity purification sequencing (DAP-seq) is described, a high-throughput TF binding site discovery method that interrogates genomic DNA with in-vitro-expressed TFs and determined that >75% of Arabidopsis TFs surveyed were methylation sensitive, a property that strongly impacts the epicistrome landscape.

TL;DR: DAP-seq is developed, a transcription factor (TF)-binding site (TFBS) discovery assay that couples affinity-purified TFs with next-generation sequencing of a genomic DNA library, enabling low-cost, high-throughput generation of cistrome and epicistrome maps for any organism.

TL;DR: The primary motor cortex (M1) is essential for voluntary fine-motor control and is functionally conserved across mammals using high-throughput transcriptomic and epigenomic profiling of more than 450k single nuclei in humans, marmoset monkeys and mice as mentioned in this paper.

TL;DR: This study reveals a unified molecular genetic landscape of cortical cell types that congruently integrates their transcriptome, open chromatin and DNA methylation maps, and establishes a unified and mechanistic framework of neuronal cell type organization that integrates multi-layered molecular genetic and spatial information with multi-faceted phenotypic properties.