Mapping Protein-Protein Interactions for the Yeast ABC Transporter Ycf1p by Integrated Split-Ubiquitin Membrane Yeast Two-Hybrid Analysis
Christian M. Paumi,Javier Menendez,Anthony Arnoldo,Kim Engels,Kavitha Ravee Iyer,Safia Thaminy,Oleg Georgiev,Yves Barral,Susan Michaelis,Igor Stagljar +9 more
TLDR
This study reports the development of a modified version of the split-ubiquitin membrane yeast two-hybrid technology using genomically integrated "bait" constructs and provides proof of principle that iMYTH is an ideal methodology to identify physiological interactors and regulators of ABC transporters and other yeast transmembrane proteins.About:
This article is published in Molecular Cell.The article was published on 2007-04-13 and is currently open access. It has received 112 citations till now. The article focuses on the topics: ATP-binding domain of ABC transporters & ATP-binding cassette transporter.read more
Citations
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Universal strategies in research and drug discovery based on protein-fragment complementation assays
TL;DR: Experimental strategies — based on protein-fragment complementation assays (PCAs) — that can achieve the direct detection of the dynamics of protein–protein interactions can be used to probe the effects of any perturbation on any pathway of interest are described.
Journal ArticleDOI
How Saccharomyces cerevisiae copes with toxic metals and metalloids
TL;DR: A review of the molecular mechanisms that contribute to metal toxicity, detoxification and tolerance acquisition in budding yeast Saccharomyces cerevisiae focuses on the metals/metalloids arsenic, cadmium, antimony, mercury, chromium and selenium.
Journal ArticleDOI
Interaction landscape of membrane - protein complexes in Saccharomyces cerevisiae
Mohan Babu,James Vlasblom,Shuye Pu,Xinghua Guo,Chris Graham,Björn D. M. Bean,Helen E. Burston,Franco J. Vizeacoumar,Jamie Snider,Sadhna Phanse,Vincent Fong,Yuen Yi C. Tam,Michael Davey,Olha Hnatshak,Navgeet Bajaj,Shamanta Chandran,Thanuja Punna,Constantine Christopolous,Victoria Wong,Analyn Yu,Gouqing Zhong,Joyce Li,Igor Stagljar,Elizabeth Conibear,Shoshana J. Wodak,Andrew Emili,Jack Greenblatt +26 more
TL;DR: A complete survey of putative integral, peripheral and lipid-anchored MPs from Saccharomyces cerevisiae was reported, which revealed unexpected physical associations underlying the membrane biology of eukaryotes and delineates the global topological landscape of the membrane interactome.
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mCSM-PPI2: predicting the effects of mutations on protein-protein interactions.
Carlos H M Rodrigues,Carlos H M Rodrigues,Yoochan Myung,Yoochan Myung,Douglas E. V. Pires,Douglas E. V. Pires,David B. Ascher +6 more
TL;DR: McSm-PPI2 as mentioned in this paper uses graph-based structural signatures to model effects of variations on the inter-residue interaction network, evolutionary information, complex network metrics and energetic terms to generate an optimised predictor.
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Fundamentals of protein interaction network mapping
TL;DR: This review discusses the strengths and weaknesses of individual assays, how to select a method appropriate for the problem being studied, and general guidelines for carrying out the necessary follow-up analyses of interactome mapping.
References
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Functional organization of the yeast proteome by systematic analysis of protein complexes
Anne-Claude Gavin,Markus Bösche,Roland Krause,Paola Grandi,Martina Marzioch,Andreas Bauer,Jörg Schultz,Jens Rick,Anne-Marie Michon,Cristina-Maria Cruciat,Marita Remor,Christian Höfert,Malgorzata Schelder,Miro Brajenovic,Heinz Ruffner,Alejandro Merino,Karin Klein,Manuela Hudak,David Dickson,Tatjana Rudi,Volker Gnau,Angela Bauch,Sonja Bastuck,Bettina Huhse,Christina Leutwein,Marie-Anne Heurtier,Richard R. Copley,Angela Edelmann,Erich Querfurth,Vladimir Rybin,Gerard Drewes,Manfred Raida,Tewis Bouwmeester,Peer Bork,Bertrand Séraphin,Bernhard Kuster,Gitte Neubauer,Giulio Superti-Furga +37 more
TL;DR: The analysis provides an outline of the eukaryotic proteome as a network of protein complexes at a level of organization beyond binary interactions, which contains fundamental biological information and offers the context for a more reasoned and informed approach to drug discovery.
Journal ArticleDOI
Global analysis of protein expression in yeast
Sina Ghaemmaghami,Won-Ki Huh,Kiowa Bower,Russell W. Howson,Archana Belle,Noah Dephoure,Erin K. O'Shea,Jonathan S. Weissman +7 more
TL;DR: A Saccharomyces cerevisiae fusion library is created where each open reading frame is tagged with a high-affinity epitope and expressed from its natural chromosomal location, and it is found that about 80% of the proteome is expressed during normal growth conditions.
Journal ArticleDOI
A comprehensive two-hybrid analysis to explore the yeast protein interactome
TL;DR: The comprehensive analysis using a system to examine two-hybrid interactions in all possible combinations between the budding yeast Saccharomyces cerevisiae is completed and would significantly expand and improve the protein interaction map for the exploration of genome functions that eventually leads to thorough understanding of the cell as a molecular system.
Journal ArticleDOI
Proteome survey reveals modularity of the yeast cell machinery
Anne-Claude Gavin,Patrick Aloy,Paola Grandi,Roland Krause,Markus Boesche,Martina Marzioch,Christina Rau,Lars Juhl Jensen,Sonja Bastuck,Birgit Dümpelfeld,Angela Edelmann,Marie-Anne Heurtier,Verena Hoffman,Christian Hoefert,Karin Klein,Manuela Hudak,Anne-Marie Michon,Malgorzata Schelder,Markus Schirle,Marita Remor,Tatjana Rudi,Sean D. Hooper,Andreas Bauer,Tewis Bouwmeester,Georg Casari,Gerard Drewes,Gitte Neubauer,Jens Rick,Bernhard Kuster,Peer Bork,Robert B. Russell,Giulio Superti-Furga +31 more
TL;DR: This study reports the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry and provides the largest collection of physically determined eukaryotic cellular machines so far and a platform for biological data integration and modelling.
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Creating new fluorescent probes for cell biology.
TL;DR: Advances include the continued development of 'passive' markers for the measurement of biomolecule expression and localization in live cells, and 'active' indicators for monitoring more complex cellular processes such as small-molecule-messenger dynamics, enzyme activation and protein–protein interactions.
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