MicroRNA-199a-3p is downregulated in human osteosarcoma and regulates cell proliferation and migration.
Zhenfeng Duan,Edwin Choy,David C. Harmon,Xianzhe Liu,Michiro Susa,Henry J. Mankin,Francis J. Hornicek +6 more
TLDR
New insights for miRNAs in osteosarcoma are provided and it is suggested that miR-199a-3p may play a functional role in osteOSARcoma cell growth and proliferation.Abstract:
microRNAs (miRNA, miR) play an important role in cancer cell growth and migration; however, the potential roles of miRNAs in osteosarcoma remain largely uncharacterized. By applying a miRNA microarray platform and unsupervised hierarchical clustering analysis, we found that several miRNAs have altered expression levels in osteosarcoma cell lines and tumor tissues when compared with normal human osteoblasts. Three miRNAs, miR-199a-3p, miR-127-3p, and miR-376c, were significantly decreased in osteosarcoma cell lines, whereas miR-151-3p and miR-191 were increased in osteosarcoma cell lines in comparison with osteoblasts. Transfection of precursor miR-199a-3p into osteosarcoma cell lines significantly decreased cell growth and migration, thus indicating that the inhibition effect is associated with an increase in the G1-phase and a decrease of the S-phase cell population. In addition, we observed decreased mTOR and Stat3 expression in miR-199a-3p transfected cells. This study provides new insights for miRNAs in osteosarcoma and suggests that miR-199a-3p may play a functional role in osteosarcoma cell growth and proliferation. Restoring miR-199a-3p's function may provide therapeutic benefits in osteosarcoma. Mol Cancer Ther; 10(8); 1337–45. ©2011 AACR .read more
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DissertationDOI
Identification of miRNAs with impact on melanoma cell invasion
TL;DR: Findings indicate that miR-339-3p can act as a tumor suppressor in melanoma and the extent of its expression levels might contribute to the varying aggressiveness of different melanomas.
Journal Article
Effect of miR-135b inhibitor on biological characteristics of osteosarcoma cells through up-regulating PPM1A.
TL;DR: MiR-135b is up-regulated in OS cells and down regulating miR- 135b expression could inhibit the proliferation and invasion of OS cells by up-regulating PPM1A.
Journal ArticleDOI
MicroRNAs and exosomes: Cardiac stem cells in heart diseases
TL;DR: In this article , the role of miRNAs and exosomes in the regulation of cardiac stem cells' behavior is discussed. But, the authors do not discuss the effect of miRNA on the function of the exosome.
Book ChapterDOI
MicroRNAs in Bone Diseases: Progress and Prospects
TL;DR: The role of miRNAs in normal osteoblast and osteosarcoma cells is focused on and the great potential of miRNA as a new therapeutic approach to treat human bone diseases will also be discussed.
Journal ArticleDOI
Changes in morphology and miRNAs expression in small intestines of Shaoxing ducks in response to high temperature
TL;DR: The results demonstrated that varying degrees of damage to each intestinal segment were found in heat-treated ducks, and there were more severe injuries in duodenum and jejunum than those in ileum, suggesting the involvement of specific molecular mechanisms of post-transcriptional regulation to explain the high temperature-induced changes in the duck small intestine.
References
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Journal Article
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Journal ArticleDOI
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