MicroRNA-199a-3p is downregulated in human osteosarcoma and regulates cell proliferation and migration.
Zhenfeng Duan,Edwin Choy,David C. Harmon,Xianzhe Liu,Michiro Susa,Henry J. Mankin,Francis J. Hornicek +6 more
TLDR
New insights for miRNAs in osteosarcoma are provided and it is suggested that miR-199a-3p may play a functional role in osteOSARcoma cell growth and proliferation.Abstract:
microRNAs (miRNA, miR) play an important role in cancer cell growth and migration; however, the potential roles of miRNAs in osteosarcoma remain largely uncharacterized. By applying a miRNA microarray platform and unsupervised hierarchical clustering analysis, we found that several miRNAs have altered expression levels in osteosarcoma cell lines and tumor tissues when compared with normal human osteoblasts. Three miRNAs, miR-199a-3p, miR-127-3p, and miR-376c, were significantly decreased in osteosarcoma cell lines, whereas miR-151-3p and miR-191 were increased in osteosarcoma cell lines in comparison with osteoblasts. Transfection of precursor miR-199a-3p into osteosarcoma cell lines significantly decreased cell growth and migration, thus indicating that the inhibition effect is associated with an increase in the G1-phase and a decrease of the S-phase cell population. In addition, we observed decreased mTOR and Stat3 expression in miR-199a-3p transfected cells. This study provides new insights for miRNAs in osteosarcoma and suggests that miR-199a-3p may play a functional role in osteosarcoma cell growth and proliferation. Restoring miR-199a-3p's function may provide therapeutic benefits in osteosarcoma. Mol Cancer Ther; 10(8); 1337–45. ©2011 AACR .read more
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The microRNAs within the DLK1-DIO3 genomic region: involvement in disease pathogenesis.
Leonidas Benetatos,Eleftheria Hatzimichael,Eleftheria Hatzimichael,Eric Londin,George Vartholomatos,Phillipe Loher,Isidore Rigoutsos,Evangelos Briasoulis +7 more
TL;DR: An in-depth review of this vital genomic region is presented, and the role the microRNAs of this region may play in controlling tissue homeostasis and in the pathogenesis of some human diseases, mostly cancer, when aberrantly expressed is examined.
Journal ArticleDOI
miR-30a-5p suppresses breast tumor growth and metastasis through inhibition of LDHA-mediated Warburg effect.
Ling Li,Lei Kang,Wei Zhao,Yingying Feng,Wenpeng Liu,Tao Wang,Haixing Mai,Jun Huang,Siyu Chen,Yingchun Liang,Juqiang Han,Xiaojie Xu,Qinong Ye +12 more
TL;DR: Through inhibition of LDHA, miR-30a-5p dampens glycolysis by decreasing glucose uptake, lactate production, ATP generation, and extracellular acidification rate (ECAR), and increasing oxygen consumption rate (OCR) in breast cancer cells.
Journal ArticleDOI
MicroRNA Involvement in Osteosarcoma
TL;DR: The current understanding of the roles that miRNAs play in OS is summarized, their potential as biomarkers or therapeutic targets are highlighted, and there are several challenges that must be addressed in order to translate miRNA-based therapeutics to the clinical setting.
Journal ArticleDOI
MicroRNA in intervertebral disc degeneration.
TL;DR: Current research enlightenment towards understanding roles of microRNAs in regulating nucleus pulposus cell functions in IDD is summarized, supporting the notion that specific modulation of micro RNAs may represent an attractive approach for management of IDD.
Journal ArticleDOI
MicroRNAs and Potential Targets in Osteosarcoma: Review
TL;DR: It is suggested a global approach to the understanding of the pathogenesis of osteosarcoma may identify candidate miRNAs as promising biomarkers for this rare disease.
References
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Journal ArticleDOI
MiR-199a-3p Regulates mTOR and c-Met to Influence the Doxorubicin Sensitivity of Human Hepatocarcinoma Cells
Francesca Fornari,Maddalena Milazzo,Pasquale Chieco,Massimo Negrini,George A. Calin,Gian Luca Grazi,Daniela Pollutri,Carlo M. Croce,Luigi Bolondi,Laura Gramantieri +9 more
TL;DR: In vitro findings suggest that tactics to regulate mTOR and c-Met by elevating levels of miR-199a-3p may have therapeutic benefits in highly lethal cancers such as HCC.
Journal ArticleDOI
Correlation and quantitation of microRNA aberrant expression in tissues and sera from patients with breast tumor
TL;DR: A high correlation of miRNA expression level was found between breast tumor tissues and sera and should encourage further studies on the use of miRNAs in serum samples as an easy and convenient method of breast cancer screening.
Journal ArticleDOI
MicroRNA-1/206 targets c-Met and inhibits rhabdomyosarcoma development.
Dongsheng Yan,Dongsheng Yan,Xiang Da Eric Dong,Xiaoyan Chen,Xiaoyan Chen,Lihua Wang,Lihua Wang,Chunjing Lu,Chunjing Lu,Jiao Wang,Jiao Wang,Jia Qu,Jia Qu,LiLi Tu,LiLi Tu +14 more
TL;DR: The results demonstrated that miR-1/206 could function as a potent tumor suppressor in c-Met-overexpressing tumors and could contribute to aberrant cell proliferation and migration, leading to rhabdomyosarcoma development.
Journal ArticleDOI
Therapy for osteosarcoma: where do we go from here?
TL;DR: This protocol is designed to clarify whether the addition of ifosfamide and etoposide to postoperative chemotherapy with cisplatin, doxorubicin, and methotrexate improves the event-free survival and overall survival for patients with resectable osteosarcoma and a poor histologic response to 10 weeks of preoperative chemotherapy.
Journal ArticleDOI
MicroRNA expression signature of human sarcomas.
Subree Subramanian,Weng-Onn Lui,C-H Lee,C-H Lee,Inigo Espinosa,Torsten O. Nielsen,Michael Heinrich,Christopher L. Corless,Andrew Fire,M van de Rijn +9 more
TL;DR: Different histological types of sarcoma have distinct miRNA expression patterns, reflecting the apparent lineage and differentiation status of the tumors, and the identification of unique miRNA signatures in each tumor type may indicate their role in tumorigenesis and may aid in diagnosis of soft tissue sarcomas.