MicroRNA-199a-3p is downregulated in human osteosarcoma and regulates cell proliferation and migration.
Zhenfeng Duan,Edwin Choy,David C. Harmon,Xianzhe Liu,Michiro Susa,Henry J. Mankin,Francis J. Hornicek +6 more
TLDR
New insights for miRNAs in osteosarcoma are provided and it is suggested that miR-199a-3p may play a functional role in osteOSARcoma cell growth and proliferation.Abstract:
microRNAs (miRNA, miR) play an important role in cancer cell growth and migration; however, the potential roles of miRNAs in osteosarcoma remain largely uncharacterized. By applying a miRNA microarray platform and unsupervised hierarchical clustering analysis, we found that several miRNAs have altered expression levels in osteosarcoma cell lines and tumor tissues when compared with normal human osteoblasts. Three miRNAs, miR-199a-3p, miR-127-3p, and miR-376c, were significantly decreased in osteosarcoma cell lines, whereas miR-151-3p and miR-191 were increased in osteosarcoma cell lines in comparison with osteoblasts. Transfection of precursor miR-199a-3p into osteosarcoma cell lines significantly decreased cell growth and migration, thus indicating that the inhibition effect is associated with an increase in the G1-phase and a decrease of the S-phase cell population. In addition, we observed decreased mTOR and Stat3 expression in miR-199a-3p transfected cells. This study provides new insights for miRNAs in osteosarcoma and suggests that miR-199a-3p may play a functional role in osteosarcoma cell growth and proliferation. Restoring miR-199a-3p's function may provide therapeutic benefits in osteosarcoma. Mol Cancer Ther; 10(8); 1337–45. ©2011 AACR .read more
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Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A.
TL;DR: Exosomes from miR-199-3p-modified ADSCs promote proliferation and migration of endothelial tip cells by downregulation of sema3A, and the effect of Exos199 mimic on the protein expression of TIMP3, MMP9 and PCNA were negated by upregulation of Sema3a.
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Gene and cell delivery for intervertebral disc degeneration
TL;DR: In this paper, Gene and cell delivery for intervertebral disc degeneration was proposed for intervebral disc deformation, and the results showed that the cell delivery was beneficial.
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Roles of MicroRNAs in Osteogenesis or Adipogenesis Differentiation of Bone Marrow Stromal Progenitor Cells.
TL;DR: In this article, the authors present an emerging concept of microRNA regulation in the bone/fat formation switch in bone marrow, the evidence for which is assembled mainly from in vivo and in vitro human or animal models.
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MeCP2 controls neural stem cell fate specification through miR-199a-mediated inhibition of BMP-Smad signaling
Hideyuki Nakashima,Keita Tsujimura,Koichiro Irie,Takuya Imamura,Cleber A. Trujillo,Masataka Ishizu,Masahiro Uesaka,Miao Pan,Hirofumi Noguchi,Kanako Okada,Kei Aoyagi,Tomoko Andoh-Noda,Hideyuki Okano,Alysson R. Muotri,Kinichi Nakashima +14 more
TL;DR: In this article, the MeCP2/miR-199a axis regulates neural stem/precursor cell (NS/PC) differentiation, and a shift occurs from neuronal to astrocytic differentiation due to the upregulation of a miR- 199a target, Smad1, a downstream transcription factor of bone morphogenetic protein (BMP) signaling.
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Circular RNA circNRIP1 plays oncogenic roles in the progression of osteosarcoma
TL;DR: In this article, the role of circNRIP1 in osteosarcoma and explored its possible underlying mechanisms were investigated, which indicated that aberrant expression of circRNAs is associated with the occurrence and progression of many cancers.
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Journal ArticleDOI
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Journal ArticleDOI
Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability.
François Denizot,Rita Lang +1 more
TL;DR: The reliability and sensitivity of the test have been increased to the point where it can in many cases replace the [3H]thymidine uptake assay to measure cell proliferation or survival in growth factor or cytotoxicity assays.
Journal Article
Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing.
TL;DR: The clonogenic assay was more sensitive when continuous drug exposures were utilized, although this was primarily related to the increased drug exposure time, and therefore it offers a valid, simple method of assessing chemosensitivity in established cell lines.
PatentDOI
MicroRNA Signatures in Human Ovarian Cancer
TL;DR: In this paper, the authors presented novel methods for the diagnosis of ovarian cancer using at least one miR selected from miR-200b, miR -200b and miR −200c.
Journal ArticleDOI
The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44
Can Liu,Kevin Kelnar,Bigang Liu,Xin Chen,Tammy Calhoun-Davis,Hangwen Li,Lubna Patrawala,Hong Yan,Collene R. Jeter,Sofia Honorio,Jason F. Wiggins,Andreas G. Bader,Randy Fagin,David F.M. Brown,Dean G. Tang +14 more
TL;DR: This study shows that miR-34a is a key negative regulator of CD44(+) prostate cancer cells and establishes a strong rationale for developing miR -34a as a novel therapeutic agent against prostate CSCs.