MicroRNA-199a-3p is downregulated in human osteosarcoma and regulates cell proliferation and migration.
Zhenfeng Duan,Edwin Choy,David C. Harmon,Xianzhe Liu,Michiro Susa,Henry J. Mankin,Francis J. Hornicek +6 more
TLDR
New insights for miRNAs in osteosarcoma are provided and it is suggested that miR-199a-3p may play a functional role in osteOSARcoma cell growth and proliferation.Abstract:
microRNAs (miRNA, miR) play an important role in cancer cell growth and migration; however, the potential roles of miRNAs in osteosarcoma remain largely uncharacterized. By applying a miRNA microarray platform and unsupervised hierarchical clustering analysis, we found that several miRNAs have altered expression levels in osteosarcoma cell lines and tumor tissues when compared with normal human osteoblasts. Three miRNAs, miR-199a-3p, miR-127-3p, and miR-376c, were significantly decreased in osteosarcoma cell lines, whereas miR-151-3p and miR-191 were increased in osteosarcoma cell lines in comparison with osteoblasts. Transfection of precursor miR-199a-3p into osteosarcoma cell lines significantly decreased cell growth and migration, thus indicating that the inhibition effect is associated with an increase in the G1-phase and a decrease of the S-phase cell population. In addition, we observed decreased mTOR and Stat3 expression in miR-199a-3p transfected cells. This study provides new insights for miRNAs in osteosarcoma and suggests that miR-199a-3p may play a functional role in osteosarcoma cell growth and proliferation. Restoring miR-199a-3p's function may provide therapeutic benefits in osteosarcoma. Mol Cancer Ther; 10(8); 1337–45. ©2011 AACR .read more
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The Study of Sarcoma Microenvironment Heterogeneity Associated With Prognosis Based on an Immunogenomic Landscape Analysis
TL;DR: The significant relationship between genetic alterations and prognostic-related TME characteristics in sarcomas were determined and may provide new clues for the treatment of sarcoma patients.
Journal ArticleDOI
Synergistic and independent action of endogenous microRNAs 122a and 199a for post-transcriptional liver detargeting of gene vectors
TL;DR: A novel dual targeted system whereby miRNA122a and miRNA199a act either synergistically or independently in regulating transgene expression with vectors harbouring binding sites of both miRNAs is presented and have implications in detargeting vectors from multiple cell types in the liver.
Journal Article
Exploration and validation of downregulated microRNA-199a-3p, downstream messenger RNA targets and transcriptional regulation in osteosarcoma
Wen-Ting Huang,An-gui Liu,Kai-Teng Cai,Rong-Quan He,Zhen Li,Qing-jun Wei,Ming-Yue Chen,Jing-Yuan Huang,Wan-Yun Yan,Hong Zhou,Gang Chen,Jie Ma +11 more
TL;DR: It is determined that miR-199a-3p may serve as a molecular marker useful for distinguishing OS tissues from normal tissues with high sensitivity and specificity, with the measured outcomes being 0.94 (95% CI: 0.80, 0.96) and 0.78, 1.00, respectively.
Journal ArticleDOI
The Molecular Genetic Expression as a Novel Biomarker in the Evaluation and Monitoring of Patients With Osteosarcoma-Subtype Bone Cancer Disease.
Delia Hutanu,Roxana Popescu,Horatiu Stefanescu,Laurentiu Pirtea,Adrian Constantin Candea,Cristi Sarau,Ovidiu Boruga,Lagtonbi Mehdi,Ioana Ciuca,Sonia Tanasescu +9 more
TL;DR: This updated work wants to present a series of changes in the expression of miRNAs in bone cancer, and the implications of mi RNAs in targeted therapy in such patients, using studies available in scientific databases such as PubMed and Scopus.
Journal ArticleDOI
Role of microRNA-92a in metastasis of osteosarcoma cells in vivo and in vitro by inhibiting expression of TCF21 with the transmission of bone marrow derived mesenchymal stem cells
TL;DR: It is demonstrated that the expression of miR-92a is high in OS and BMSCs transfers miR -92a to inhibit TCF21 and promotes growth and metastasis of OS in vitro and in vivo.
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