MicroRNA-199a-3p is downregulated in human osteosarcoma and regulates cell proliferation and migration.
Zhenfeng Duan,Edwin Choy,David C. Harmon,Xianzhe Liu,Michiro Susa,Henry J. Mankin,Francis J. Hornicek +6 more
TLDR
New insights for miRNAs in osteosarcoma are provided and it is suggested that miR-199a-3p may play a functional role in osteOSARcoma cell growth and proliferation.Abstract:
microRNAs (miRNA, miR) play an important role in cancer cell growth and migration; however, the potential roles of miRNAs in osteosarcoma remain largely uncharacterized. By applying a miRNA microarray platform and unsupervised hierarchical clustering analysis, we found that several miRNAs have altered expression levels in osteosarcoma cell lines and tumor tissues when compared with normal human osteoblasts. Three miRNAs, miR-199a-3p, miR-127-3p, and miR-376c, were significantly decreased in osteosarcoma cell lines, whereas miR-151-3p and miR-191 were increased in osteosarcoma cell lines in comparison with osteoblasts. Transfection of precursor miR-199a-3p into osteosarcoma cell lines significantly decreased cell growth and migration, thus indicating that the inhibition effect is associated with an increase in the G1-phase and a decrease of the S-phase cell population. In addition, we observed decreased mTOR and Stat3 expression in miR-199a-3p transfected cells. This study provides new insights for miRNAs in osteosarcoma and suggests that miR-199a-3p may play a functional role in osteosarcoma cell growth and proliferation. Restoring miR-199a-3p's function may provide therapeutic benefits in osteosarcoma. Mol Cancer Ther; 10(8); 1337–45. ©2011 AACR .read more
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LncRNA CCAT2 promoted osteosarcoma cell proliferation and invasion
TL;DR: Overexpression of CCAT2 promoted osteosarcoma cell proliferation, invasion and cell cycle and promotion of mesenchymal markers N‐cadherin, vimentin and snail and reduced the expression of N‐ cadher in marker E‐c Cadherin.
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MiR-199a/b-3p suppresses migration and invasion of breast cancer cells by downregulating PAK4/MEK/ERK signaling pathway.
TL;DR: It is found that miR‐199a/b‐3p functions as a tumor suppressor and has an important role in breast cancer metastasis through PAK4/MEK/ERK signaling pathway.
Journal ArticleDOI
Nanodelivery systems for nucleic acid therapeutics in drug resistant tumors.
TL;DR: The principles and mechanisms based on the RNA interference phenomenon and the barriers to its successful clinical translation are described and illustrative examples of miRNA, siRNA, and gene-drug combination delivery using nanoparticle systems that have shown promising potentials for the treatment of diseases such as MDR cancers are covered.
Journal ArticleDOI
MicroRNA-127-3p inhibits proliferation and invasion by targeting SETD8 in human osteosarcoma cells.
TL;DR: The results revealed that miR-127-3p acts as a tumor suppressor and that its down-regulation in cancer may contribute to OS progression and metastasis, suggesting thatMiR- 127-3P could be a potential therapeutic target in the treatment of OS.
Journal ArticleDOI
Aberrant expression of miR-199a-3p and its clinical significance in colorectal cancers
Daiwei Wan,Songbing He,Binhui Xie,Guohui Xu,Wen Gu,Chenglong Shen,You Hu,Xinsheng Wang,Qiaoming Zhi,Liang Wang +9 more
TL;DR: Clinopathologic analysis revealed that high miR-199a-3p expression contributed to more advanced lymphatic invasion, lymph node metastasis, liver metastases and late TNM stage in colorectal cancer, and suggested that miR,199a,3p may serve as an efficient biomarker for diagnosis and novel prognostic indicator in colorescopy cancer.
References
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Journal ArticleDOI
Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
TL;DR: A tetrazolium salt has been used to develop a quantitative colorimetric assay for mammalian cell survival and proliferation and is used to measure proliferative lymphokines, mitogen stimulations and complement-mediated lysis.
Journal ArticleDOI
Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability.
François Denizot,Rita Lang +1 more
TL;DR: The reliability and sensitivity of the test have been increased to the point where it can in many cases replace the [3H]thymidine uptake assay to measure cell proliferation or survival in growth factor or cytotoxicity assays.
Journal Article
Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing.
TL;DR: The clonogenic assay was more sensitive when continuous drug exposures were utilized, although this was primarily related to the increased drug exposure time, and therefore it offers a valid, simple method of assessing chemosensitivity in established cell lines.
PatentDOI
MicroRNA Signatures in Human Ovarian Cancer
TL;DR: In this paper, the authors presented novel methods for the diagnosis of ovarian cancer using at least one miR selected from miR-200b, miR -200b and miR −200c.
Journal ArticleDOI
The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44
Can Liu,Kevin Kelnar,Bigang Liu,Xin Chen,Tammy Calhoun-Davis,Hangwen Li,Lubna Patrawala,Hong Yan,Collene R. Jeter,Sofia Honorio,Jason F. Wiggins,Andreas G. Bader,Randy Fagin,David F.M. Brown,Dean G. Tang +14 more
TL;DR: This study shows that miR-34a is a key negative regulator of CD44(+) prostate cancer cells and establishes a strong rationale for developing miR -34a as a novel therapeutic agent against prostate CSCs.