MicroRNA-199a-3p is downregulated in human osteosarcoma and regulates cell proliferation and migration.
Zhenfeng Duan,Edwin Choy,David C. Harmon,Xianzhe Liu,Michiro Susa,Henry J. Mankin,Francis J. Hornicek +6 more
TLDR
New insights for miRNAs in osteosarcoma are provided and it is suggested that miR-199a-3p may play a functional role in osteOSARcoma cell growth and proliferation.Abstract:
microRNAs (miRNA, miR) play an important role in cancer cell growth and migration; however, the potential roles of miRNAs in osteosarcoma remain largely uncharacterized. By applying a miRNA microarray platform and unsupervised hierarchical clustering analysis, we found that several miRNAs have altered expression levels in osteosarcoma cell lines and tumor tissues when compared with normal human osteoblasts. Three miRNAs, miR-199a-3p, miR-127-3p, and miR-376c, were significantly decreased in osteosarcoma cell lines, whereas miR-151-3p and miR-191 were increased in osteosarcoma cell lines in comparison with osteoblasts. Transfection of precursor miR-199a-3p into osteosarcoma cell lines significantly decreased cell growth and migration, thus indicating that the inhibition effect is associated with an increase in the G1-phase and a decrease of the S-phase cell population. In addition, we observed decreased mTOR and Stat3 expression in miR-199a-3p transfected cells. This study provides new insights for miRNAs in osteosarcoma and suggests that miR-199a-3p may play a functional role in osteosarcoma cell growth and proliferation. Restoring miR-199a-3p's function may provide therapeutic benefits in osteosarcoma. Mol Cancer Ther; 10(8); 1337–45. ©2011 AACR .read more
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The microRNAs within the DLK1-DIO3 genomic region: involvement in disease pathogenesis.
Leonidas Benetatos,Eleftheria Hatzimichael,Eleftheria Hatzimichael,Eric Londin,George Vartholomatos,Phillipe Loher,Isidore Rigoutsos,Evangelos Briasoulis +7 more
TL;DR: An in-depth review of this vital genomic region is presented, and the role the microRNAs of this region may play in controlling tissue homeostasis and in the pathogenesis of some human diseases, mostly cancer, when aberrantly expressed is examined.
Journal ArticleDOI
miR-30a-5p suppresses breast tumor growth and metastasis through inhibition of LDHA-mediated Warburg effect.
Ling Li,Lei Kang,Wei Zhao,Yingying Feng,Wenpeng Liu,Tao Wang,Haixing Mai,Jun Huang,Siyu Chen,Yingchun Liang,Juqiang Han,Xiaojie Xu,Qinong Ye +12 more
TL;DR: Through inhibition of LDHA, miR-30a-5p dampens glycolysis by decreasing glucose uptake, lactate production, ATP generation, and extracellular acidification rate (ECAR), and increasing oxygen consumption rate (OCR) in breast cancer cells.
Journal ArticleDOI
MicroRNA Involvement in Osteosarcoma
TL;DR: The current understanding of the roles that miRNAs play in OS is summarized, their potential as biomarkers or therapeutic targets are highlighted, and there are several challenges that must be addressed in order to translate miRNA-based therapeutics to the clinical setting.
Journal ArticleDOI
MicroRNA in intervertebral disc degeneration.
TL;DR: Current research enlightenment towards understanding roles of microRNAs in regulating nucleus pulposus cell functions in IDD is summarized, supporting the notion that specific modulation of micro RNAs may represent an attractive approach for management of IDD.
Journal ArticleDOI
MicroRNAs and Potential Targets in Osteosarcoma: Review
TL;DR: It is suggested a global approach to the understanding of the pathogenesis of osteosarcoma may identify candidate miRNAs as promising biomarkers for this rare disease.
References
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Twist-1 regulates the miR-199a/214 cluster during development
Youn-Bok Lee,Ioannis Bantounas,Do-Young Lee,Leonidas A. Phylactou,Maeve A. Caldwell,James B. Uney +5 more
TL;DR: This study shows the expression of the miR199a/214 cluster is controlled by Twist-1 via an E-Box promoter element and supports a role for these miRNAs as novel intermediates in the pathways controlling the development of specific neural cell populations.
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MicroRNA miR-199a* Regulates the MET Proto-oncogene and the Downstream Extracellular Signal-regulated Kinase 2 (ERK2)
Seonhoe Kim,Ui Jin Lee,Mi Na Kim,Eun-Ju Lee,Jiyoung Kim,Mi-Young Lee,Sorim Choung,Young-Joo Kim,Young Chul Choi +8 more
TL;DR: Coordinated down-regulation of both MET and its downstream effector ERK2 by miR-199a* may be effective in inhibiting not only cell proliferation but also motility and invasive capabilities of tumor cells.
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Tumor suppression by RNA from the 3′ untranslated region of α-tropomyosin
TL;DR: It is concluded that untranslated RNAs can function as regulators (riboregulators) that suppress tumor formation in NMU2 cells and that suppression of tumorigenicity is dependent on the continued expression of Tm transcripts lacking a coding region.
Journal ArticleDOI
MicroRNAs Impair MET-Mediated Invasive Growth
Cristina Migliore,Annalisa Petrelli,Elena Ghiso,Simona Corso,Lorena Capparuccia,Adriana Eramo,Paolo M. Comoglio,Silvia Giordano +7 more
TL;DR: This work has identified miRNAs that behave as oncosuppressors by negatively targeting MET and might thus provide an additional option to inhibit this oncogene in tumors displaying its deregulation.
Journal ArticleDOI
Downregulation of the Rho GTPase signaling pathway is involved in the microRNA-138-mediated inhibition of cell migration and invasion in tongue squamous cell carcinoma.
Lu Jiang,Xiqiang Liu,Xiqiang Liu,Antonia Kolokythas,Jinsheng Yu,Anxun Wang,Anxun Wang,Caroline E. Heidbreder,Fei Shi,Xiaofeng Zhou +9 more
TL;DR: The results suggest that miR‐138 plays an important role in TSCC cell migration and invasion by concurrently targeting RhoC and ROCK2, and miR-138 may serve as a novel therapeutic target for TSCC patients at risk of metastatic disease.