MicroRNA-199a-3p is downregulated in human osteosarcoma and regulates cell proliferation and migration.
Zhenfeng Duan,Edwin Choy,David C. Harmon,Xianzhe Liu,Michiro Susa,Henry J. Mankin,Francis J. Hornicek +6 more
TLDR
New insights for miRNAs in osteosarcoma are provided and it is suggested that miR-199a-3p may play a functional role in osteOSARcoma cell growth and proliferation.Abstract:
microRNAs (miRNA, miR) play an important role in cancer cell growth and migration; however, the potential roles of miRNAs in osteosarcoma remain largely uncharacterized. By applying a miRNA microarray platform and unsupervised hierarchical clustering analysis, we found that several miRNAs have altered expression levels in osteosarcoma cell lines and tumor tissues when compared with normal human osteoblasts. Three miRNAs, miR-199a-3p, miR-127-3p, and miR-376c, were significantly decreased in osteosarcoma cell lines, whereas miR-151-3p and miR-191 were increased in osteosarcoma cell lines in comparison with osteoblasts. Transfection of precursor miR-199a-3p into osteosarcoma cell lines significantly decreased cell growth and migration, thus indicating that the inhibition effect is associated with an increase in the G1-phase and a decrease of the S-phase cell population. In addition, we observed decreased mTOR and Stat3 expression in miR-199a-3p transfected cells. This study provides new insights for miRNAs in osteosarcoma and suggests that miR-199a-3p may play a functional role in osteosarcoma cell growth and proliferation. Restoring miR-199a-3p's function may provide therapeutic benefits in osteosarcoma. Mol Cancer Ther; 10(8); 1337–45. ©2011 AACR .read more
Citations
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Performance Evaluation and Online Realization of Data-driven Normalization Methods Used in LC/MS based Untargeted Metabolomics Analysis.
Bo Li,Jing Tang,Qingxia Yang,Xuejiao Cui,Shuang Li,Sijie Chen,Quanxing Cao,Weiwei Xue,Na Chen,Feng Zhu +9 more
TL;DR: This study could serve as a useful guidance to the selection of suitable normalization methods in analyzing the LC/MS based metabolomics data.
Journal ArticleDOI
A three-plasma miRNA signature serves as novel biomarkers for osteosarcoma
TL;DR: The data suggest that altered levels of circulating miRNAs might have great potential to serve as novel, non-invasive biomarkers for osteosarcoma.
Journal ArticleDOI
Detection of spontaneous tumorigenic transformation during culture expansion of human mesenchymal stromal cells.
Qiuwei Pan,Suomi M. G. Fouraschen,Petra E. de Ruiter,Winand N.M. Dinjens,Jaap Kwekkeboom,Hugo W. Tilanus,Luc J. W. van der Laan +6 more
TL;DR: The spontaneous transformation of MSCs resulting in tumorigenesis is rare and occurs after relatively long-term (beyond five weeks) culture, however, as an added safety measure, cultures of M SCs can potentially be screened based on a novel gene expression signature.
Journal ArticleDOI
Identification of MicroRNAs associated with early relapse after nephrectomy in renal cell carcinoma patients.
Ondrej Slaby,Martina Rédová,Alexandr Poprach,Jana Nekvindová,Robert Iliev,Lenka Radová,Radek Lakomy,Marek Svoboda,Rostislav Vyzula +8 more
TL;DR: It is suggested that identified miRNAs might be used for identification of RCC patients at high risk of early relapse after nephrectomy in clinical practice.
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MicroRNA-340 suppresses osteosarcoma tumor growth and metastasis by directly targeting ROCK1.
TL;DR: Findings indicate that miR-340 acts as a tumor suppressor and its downregulation in tumor tissues may contribute to the progression and metastasis of osteosarcoma through a mechanism involving ROCK1, suggesting mi R-340 as a potential new diagnostic and therapeutic target for the treatment of OS.
References
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The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44
Can Liu,Kevin Kelnar,Bigang Liu,Xin Chen,Tammy Calhoun-Davis,Hangwen Li,Lubna Patrawala,Hong Yan,Collene R. Jeter,Sofia Honorio,Jason F. Wiggins,Andreas G. Bader,Randy Fagin,David F.M. Brown,Dean G. Tang +14 more
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