MicroRNA-199a-3p is downregulated in human osteosarcoma and regulates cell proliferation and migration.
Zhenfeng Duan,Edwin Choy,David C. Harmon,Xianzhe Liu,Michiro Susa,Henry J. Mankin,Francis J. Hornicek +6 more
TLDR
New insights for miRNAs in osteosarcoma are provided and it is suggested that miR-199a-3p may play a functional role in osteOSARcoma cell growth and proliferation.Abstract:
microRNAs (miRNA, miR) play an important role in cancer cell growth and migration; however, the potential roles of miRNAs in osteosarcoma remain largely uncharacterized. By applying a miRNA microarray platform and unsupervised hierarchical clustering analysis, we found that several miRNAs have altered expression levels in osteosarcoma cell lines and tumor tissues when compared with normal human osteoblasts. Three miRNAs, miR-199a-3p, miR-127-3p, and miR-376c, were significantly decreased in osteosarcoma cell lines, whereas miR-151-3p and miR-191 were increased in osteosarcoma cell lines in comparison with osteoblasts. Transfection of precursor miR-199a-3p into osteosarcoma cell lines significantly decreased cell growth and migration, thus indicating that the inhibition effect is associated with an increase in the G1-phase and a decrease of the S-phase cell population. In addition, we observed decreased mTOR and Stat3 expression in miR-199a-3p transfected cells. This study provides new insights for miRNAs in osteosarcoma and suggests that miR-199a-3p may play a functional role in osteosarcoma cell growth and proliferation. Restoring miR-199a-3p's function may provide therapeutic benefits in osteosarcoma. Mol Cancer Ther; 10(8); 1337–45. ©2011 AACR .read more
Citations
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Circulating miR-627-5p and miR-199a-5p are promising diagnostic biomarkers of colorectal neoplasia
TL;DR: Serum levels of miR-199a-5p andmiR-627- 5p were markedly increased in patients with colorectal neoplasms and showed strong potential as minimally invasive biomarkers for the early screening of colore CT neoplasm.
Journal ArticleDOI
Small is the New Big - Interplay of Mirnas in Cancer
TL;DR: A comprehensive view of various cancer types and their associated microRNAs is provided and a key role in all cellular functions is implicated in most diseases, including cancer.
DissertationDOI
Trypanosoma brucei: Protein Expression Microarrays and Circulating miRNA during Infection
TL;DR: There is deregulation of miRNA expression following T. brucei infection, and most of the differentially regulated miRNAs are related to immune responses to infectious agents and other inflammatory responses influencing disease outcome.
Journal ArticleDOI
High expression of microRNA‑127 is involved in cell cycle arrest in MC‑3 mucoepidermoid carcinoma cells.
TL;DR: In this article, the importance of miR-127-3p in MC-3 human MEC cells was analyzed using 3.5-dimethylthiazol-20yl, 4'6-diamidino-2-phenylindole staining, anchorage-independent cell transformation assay and western blot analysis.
Journal ArticleDOI
The hepatocyte growth factor/mesenchymal epithelial transition factor axis in high-risk pediatric solid tumors and the anti-tumor activity of targeted therapeutic agents
Megan Grundy,Aru Narendran +1 more
TL;DR: A review of the literature that explores the potential and biological rationale for targeting the HGF/c-MET pathway in common and high-risk pediatric solid tumors and selected recent and ongoing clinical trials with these agents in relapsed pediatric tumors that may provide applicable future treatments for patients.
References
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Journal ArticleDOI
The microRNA miR-34a inhibits prostate cancer stem cells and metastasis by directly repressing CD44
Can Liu,Kevin Kelnar,Bigang Liu,Xin Chen,Tammy Calhoun-Davis,Hangwen Li,Lubna Patrawala,Hong Yan,Collene R. Jeter,Sofia Honorio,Jason F. Wiggins,Andreas G. Bader,Randy Fagin,David F.M. Brown,Dean G. Tang +14 more
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