Olfactory neural circuitry for attraction to amino acids revealed by transposon-mediated gene trap approach in zebrafish
Tetsuya Koide,Nobuhiko Miyasaka,Kozo Morimoto,Kazuhide Asakawa,Akihiro Urasaki,Koichi Kawakami,Koichi Kawakami,Yoshihiro Yoshihara +7 more
TLDR
The integrated approach combining genetic, neuroanatomical, and behavioral methods enables us to elucidate the neural circuit mechanism underlying various olfactory behaviors in adult zebrafish.Abstract:Â
In fish, amino acids are food-related important olfactory cues to elicit an attractive response. However, the neural circuit underlying this olfactory behavior is not fully elucidated. In the present study, we applied the Tol2 transposon-mediated gene trap method to dissect the zebrafish olfactory system genetically. Four zebrafish lines (SAGFF27A, SAGFF91B, SAGFF179A, and SAGFF228C) were established in which the modified transcription activator Gal4FF was expressed in distinct subsets of olfactory sensory neurons (OSNs). The OSNs in individual lines projected axons to partially overlapping but mostly different glomeruli in the olfactory bulb (OB). In SAGFF27A, Gal4FF was expressed predominantly in microvillous OSNs innervating the lateral glomerular cluster that corresponded to the amino acid-responsive region in the OB. To clarify the olfactory neural pathway mediating the feeding behavior, we genetically expressed tetanus neurotoxin in the Gal4FF lines to block synaptic transmission in distinct populations of glomeruli and examined their behavioral response to amino acids. The attractive response to amino acids was abolished only in SAGFF27A fish carrying the tetanus neurotoxin transgene. These findings clearly demonstrate the functional significance of the microvillous OSNs innervating the lateral glomerular cluster in the amino acid-mediated feeding behavior of zebrafish. Thus, the integrated approach combining genetic, neuroanatomical, and behavioral methods enables us to elucidate the neural circuit mechanism underlying various olfactory behaviors in adult zebrafish.read more
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Journal ArticleDOI
Towards a Comprehensive Catalog of Zebrafish Behavior 1.0 and Beyond
Allan V. Kalueff,Michael Gebhardt,Adam Stewart,Adam Stewart,Jonathan Cachat,Mallorie Brimmer,Jonathan S Chawla,Cassandra Craddock,Evan J. Kyzar,Andrew Roth,Samuel Landsman,Siddharth Gaikwad,Kyle Robinson,Erik Baatrup,Keith B. Tierney,Angela L. Shamchuk,William H. J. Norton,Noam Miller,Teresa Nicolson,Oliver Braubach,Charles P. Gilman,Julian Pittman,Denis B. Rosemberg,Robert Gerlai,David J. Echevarria,Elisabeth Lamb,Stephan C.F. Neuhauss,Wei Weng,Laure Bally-Cuif,Henning Schneider +29 more
TL;DR: A detailed catalog of zebrafish behaviors that covers both larval and adult models is developed, representing a beginning of creating a more comprehensive ethogram ofZebrafish behavior, which will improve interpretation of published findings, foster cross-species behavioral modeling, and encourage new groups to apply zebra fish neurobehavioral paradigms in their research.
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Modeling anxiety using adult zebrafish: A conceptual review
TL;DR: This work evaluates established and novel approaches to uncover the molecular substrates, genetic pathways and neural circuits of anxiety using adult zebrafish, and provides a conceptual framework for the wider application of zebra fish and other aquatic models in anxiety research.
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Adult zebrafish as a model organism for behavioural genetics
TL;DR: Studies of adult zebrafish are now helping to uncover the genetic pathways and neural circuits that control vertebrate behaviour.
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How Is the Olfactory Map Formed and Interpreted in the Mammalian Brain
Kensaku Mori,Hitoshi Sakano +1 more
TL;DR: Recent progress in the study of the mammalian Olfactory system is reviewed, focusing on the formation of the olfactory map and the transmission of topographical information in the OB to the ofactory cortex to elicit various behaviors.
BookDOI
The Olfactory System
TL;DR: This chapter briefly sketches the recent rapid progress in understanding the structure and function of the olfactory system and some unsolved important questions regarding this system.
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