Journal ArticleDOI
RADX controls RAD51 filament dynamics to regulate replication fork stability.
Madison B. Adolph,Taha M. Mohamed,Swati Balakrishnan,Chaoyou Xue,Florian Morati,Mauro Modesti,Eric C. Greene,Walter J. Chazin,David Cortez +8 more
TLDR
In this paper, a single-strand DNA (ssDNA) binding protein that regulates DNA replication, called RADX, is defined and its mechanism of action, and it is shown that RADX directly and selectively interacts with ATP-bound RAD51, stimulates ATP hydrolysis and destabilizes RAD51 nucleofilaments.About:
This article is published in Molecular Cell.The article was published on 2021-03-04. It has received 15 citations till now. The article focuses on the topics: Replication fork reversal & DNA replication.read more
Citations
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Journal ArticleDOI
RAD52: Paradigm of Synthetic Lethality and New Developments
TL;DR: In this article, the authors have shown that mammalian RAD52 is critical for backup DNA repair pathways in HR-deficient cancer cells, which makes RAD52 an attractive target for the development of anti-cancer therapies against BRCAdeficient cancers.
Journal ArticleDOI
Replication Fork Reversal and Protection
TL;DR: A review on the key factors and mechanisms required for the remodeling and protection of stalled replication forks in mammalian cells can be found in this paper, where the authors also discuss the role of DNA damage repair proteins.
Journal ArticleDOI
RADX prevents genome instability by confining replication fork reversal to stalled forks.
Archana Krishnamoorthy,Jessica Jackson,Taha M. Mohamed,Madison B. Adolph,Alessandro Vindigni,David Cortez +5 more
TL;DR: In this article, a single-strand DNA binding protein that binds to and destabilizes RAD51 nucleofilaments, can either inhibit or promote fork reversal depending on replication stress levels.
Journal ArticleDOI
The Emerging Roles of Rad51 in Cancer and Its Potential as a Therapeutic Target
Ziyi Wang,Renxiang Jia,Linlin Wang,Qiwei Yang,Xiao-Di Hu,Qiang Fu,Xinyu Zhang,Wenya Li,Yi Ren +8 more
TL;DR: The structure, expression pattern of Rad 51 and key Rad51 mediators involved in homologous recombination are introduced and the role of Rad51 in tumor metabolism, metastasis, resistance to chemotherapeutic agents and poly-ADP ribose polymerase inhibitors is discussed.
Journal ArticleDOI
RAD51 paralogs: Expanding roles in replication stress responses and repair.
Debanjali Bhattacharya,Satyaranjan Sahoo,T. Nagraj,Suruchi Dixit,Harshit Dwivedi,Ganesh Nagaraju +5 more
TL;DR: In this article , the authors highlight the recent findings that uncovered the novel functions of RAD51 paralogs in replication fork progression, its stability, and restart and discuss RAD51-paralogs as a potential therapeutic target for cancer treatment.
References
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Journal ArticleDOI
Comparative Protein Structure Modeling Using MODELLER
Narayanan Eswar,Ben Webb,Marc A. Marti-Renom,Mallur S. Madhusudhan,David Eramian,Min-Yi Shen,Ursula Pieper,Andrej Sali +7 more
TL;DR: This unit describes how to calculate comparative models using the program MODELLER and discusses all four steps of comparative modeling, frequently observed errors, and some applications.
Journal ArticleDOI
Double-strand break repair-independent role for BRCA2 in blocking stalled replication fork degradation by MRE11
TL;DR: Using single-molecule DNA fiber analysis, it is shown that nascent replication tracts created before fork stalling with hydroxyurea are degraded in the absence of BRCA2 but are stable in wild-type cells.
Journal ArticleDOI
Insights into DNA recombination from the structure of a RAD51-BRCA2 complex
Luca Pellegrini,David S. Yu,Thomas Lo,Shubha Anand,Miyoung Lee,Tom L. Blundell,Ashok R. Venkitaraman +6 more
TL;DR: The BRC repeat mimics a motif in RAD51 that serves as an interface for oligomerization between individual RAD51 monomers, thus enabling BRCA2 to control the assembly of the RAD51 nucleoprotein filament, which is essential for strand-pairing reactions during DNA recombination.
Journal ArticleDOI
Purified human BRCA2 stimulates RAD51-mediated recombination
TL;DR: The purification of full-length BRCA2 is reported and it is shown that it both binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA).
Journal ArticleDOI
Rad51-mediated replication fork reversal is a global response to genotoxic treatments in human cells
Ralph Zellweger,Damian Dalcher,Karun Mutreja,Matteo Berti,Jonas A. Schmid,Raquel Herrador,Alessandro Vindigni,Massimo Lopes +7 more
TL;DR: Genotoxic treatments in human cells consistently induce uncoupling of replication forks and their remodeling into four-way junctions by the RAD51 recombinase.
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