Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with HhaI.
James E. Hixson,D. T. Vernier +1 more
TLDR
This work has used restriction isotyping (restriction enzyme isoform genotyping) for rapid typing of common apolipoprotein E isoforms (E2, E3, E4) and distinguished each of the isoforms by a unique combination of HhaI fragment sizes that enabled unambiguous typing of all homozygotic and heterozygotic combinations.About:
This article is published in Journal of Lipid Research.The article was published on 1990-03-01 and is currently open access. It has received 2973 citations till now. The article focuses on the topics: Restriction enzyme & Apolipoprotein E2.read more
Citations
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Journal ArticleDOI
Apolipoprotein E: high-avidity binding to beta-amyloid and increased frequency of type 4 allele in late-onset familial Alzheimer disease.
Warren J. Strittmatter,Ann M. Saunders,Donald E. Schmechel,Margaret A. Pericak-Vance,Jan J. Enghild,Guy S. Salvesen,Allen D. Roses +6 more
TL;DR: It is demonstrated that there was a highly significant association of apolipoprotein E type 4 allele (APOE-epsilon 4) and late-onset familial Alzheimer disease.
Journal ArticleDOI
Effects of Age, Sex, and Ethnicity on the Association Between Apolipoprotein E Genotype and Alzheimer Disease: A Meta-analysis
Lindsay A. Farrer,L. Adrienne Cupples,Jonathan L. Haines,Bradley T. Hyman,Walter A. Kukull,Richard Mayeux,Richard H. Myers,Margaret A. Pericak-Vance,Neil Risch,Cornelia M. van Duijn +9 more
TL;DR: The APOE∈4 allele represents a major risk factor for AD in all ethnic groups studied, across all ages between 40 and 90 years, and in both men and women.
Journal ArticleDOI
Association of apolipoprotein E allele epsilon 4 with late-onset familial and sporadic Alzheimer's disease.
Ann M. Saunders,Warren J. Strittmatter,Donald E. Schmechel,P. St. George-Hyslop,Margaret A. Pericak-Vance,S. H. Joo,B.L. Rosi,James F. Gusella,D. R. Crapper-MacLachlan,Mark J. Alberts,Christine M. Hulette,Barbara J. Crain,Dmitry Goldgaber,A. D. Roses +13 more
TL;DR: Data support the involvement of ApoE ϵ4 in the pathogenesis of late-onset familial and sporadic AD and suggest it may operate as a susceptibility gene (risk factor) for the clinical expression of AD.
Journal ArticleDOI
Oxidative damage is the earliest event in Alzheimer disease.
Akihiko Nunomura,George Perry,Gjumrakch Aliev,Keisuke Hirai,Keisuke Hirai,Atsushi Takeda,Elizabeth Balraj,Paul K. Jones,Hossein A. Ghanbari,Takafumi Wataya,Shun Shimohama,Shigeru Chiba,Craig S. Atwood,Robert B. Petersen,Mark A. Smith +14 more
TL;DR: The observations indicate that increased oxidative damage is an early event in AD that decreases with disease progression and lesion formation and suggest that AD is associated with compensatory changes that reduce damage from reactive oxygen.
Journal ArticleDOI
Common variants at MS4A4/MS4A6E, CD2AP, CD33 and EPHA1 are associated with late-onset Alzheimer's disease.
Adam C. Naj,Gyungah Jun,Gary W. Beecham,Li-San Wang,Badri N. Vardarajan,Jacqueline L. Buros,Paul Gallins,Joseph D. Buxbaum,Gail P. Jarvik,Paul K. Crane,Eric B. Larson,Thomas D. Bird,Bradley F. Boeve,Neill R. Graff-Radford,Philip L. De Jager,Philip L. De Jager,Denis A. Evans,Julie A. Schneider,Minerva M. Carrasquillo,Nilufer Ertekin-Taner,Steven G. Younkin,Carlos Cruchaga,John S. K. Kauwe,Petra Nowotny,Patricia L. Kramer,John Hardy,Matthew J. Huentelman,Amanda J. Myers,M. Michael Barmada,F. Yesim Demirci,Clinton T. Baldwin,Robert C. Green,Ekaterina Rogaeva,Peter St George-Hyslop,Peter St George-Hyslop,Steven E. Arnold,Robert W Barber,Thomas H. Beach,Eileen H. Bigio,James D. Bowen,Adam L. Boxer,James R. Burke,Nigel J. Cairns,Christopher S. Carlson,Regina M. Carney,Steven L. Carroll,Helena C. Chui,David G. Clark,Jason J. Corneveaux,Carl W. Cotman,Jeffrey L. Cummings,Charles DeCarli,Steven T. DeKosky,Ramon Diaz-Arrastia,Malcolm B. Dick,Dennis W. Dickson,William G. Ellis,Kelley Faber,Kenneth B. Fallon,Martin R. Farlow,Steven H. Ferris,Matthew P. Frosch,Douglas Galasko,Mary Ganguli,Marla Gearing,Daniel H. Geschwind,Bernardino Ghetti,John R. Gilbert,Sid Gilman,Bruno Giordani,Jonathan D. Glass,John H. Growdon,Ronald L. Hamilton,Lindy E. Harrell,Elizabeth Head,Lawrence S. Honig,Christine M. Hulette,Bradley T. Hyman,Gregory A. Jicha,Lee-Way Jin,Nancy Johnson,Jason Karlawish,Anna Karydas,Jeffrey Kaye,Ronald C. Kim,Edward H. Koo,Neil W. Kowall,James J. Lah,Allan I. Levey,Andrew P. Lieberman,Oscar L. Lopez,Wendy J. Mack,Daniel C. Marson,Frank Martiniuk,Deborah C. Mash,Eliezer Masliah,Wayne C. McCormick,Susan M. McCurry,Andrew McDavid,Ann C. McKee,W Marsel Mesulam,Bruce L. Miller,Carol A. Miller,Joshua W. Miller,Joseph E. Parisi,Daniel P. Perl,Elaine R. Peskind,Ronald C. Petersen,Wayne W. Poon,Joseph F. Quinn,Ruchita Rajbhandary,Murray A. Raskind,Barry Reisberg,John M. Ringman,Erik D. Roberson,Roger N. Rosenberg,Mary Sano,Lon S. Schneider,William P. Seeley,Michael L. Shelanski,Michael A. Slifer,Charles D. Smith,Joshua A. Sonnen,Salvatore Spina,Robert S. Stern,Rudolph E. Tanzi,John Q. Trojanowski,Juan C. Troncoso,Vivianna M. Van Deerlin,Harry V. Vinters,Jean Paul Vonsattel,Sandra Weintraub,Kathleen A. Welsh-Bohmer,Jennifer Williamson,Randall L. Woltjer,Laura B. Cantwell,Beth A. Dombroski,Duane Beekly,Kathryn L. Lunetta,Eden R. Martin,M. Ilyas Kamboh,Andrew J. Saykin,Eric M. Reiman,David A. Bennett,John C. Morris,Thomas J. Montine,Alison Goate,Deborah Blacker,Debby W. Tsuang,Hakon Hakonarson,Walter A. Kukull,Tatiana Foroud,Jonathan L. Haines,Richard Mayeux,Margaret A. Pericak-Vance,Lindsay A. Farrer,Gerard D. Schellenberg +156 more
TL;DR: The Alzheimer Disease Genetics Consortium performed a genome-wide association study of late-onset Alzheimer disease using a three-stage design consisting of a discovery stage (stage 1), two replication stages (stages 2 and 3), and both joint analysis and meta-analysis approaches were used.
References
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Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
Randall Keichi Saiki,Stephen J. Scharf,Fred A. Faloona,Kary B. Mullis,Glenn Thomas Horn,Henry A. Erlich,Norman Arnheim +6 more
TL;DR: Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia, using primer-mediated enzymatic amplification of specific beta-globin target sequences in genomic DNA, resulting in the exponential increase of target DNA copies.
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Apolipoprotein E: cholesterol transport protein with expanding role in cell biology.
TL;DR: Apolipoprotein E is a plasma protein that serves as a ligand for low density lipoprotein receptors and, through its interaction with these receptors, participates in the transport of cholesterol and other lipids among various cells of the body.
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Apolipoprotein E polymorphism and atherosclerosis.
J Davignon,R E Gregg,C F Sing +2 more
TL;DR: The relationship between the epsilon 2 allele and type III hyperlipoproteinemia provides a valuable model for the study of complex genetic interactions in the pathogenesis of hyperlipidemia and atherosclerosis.