Small RNAs analysis in CLL reveals a deregulation of miRNA expression and novel miRNA candidates of putative relevance in CLL pathogenesis.
S Marton,M R Garcia,Carlos Robello,Helena Persson,Felipe Trajtenberg,Otto Pritsch,Carlos Rovira,Hugo Naya,Guillermo Dighiero,Alfonso Cayota +9 more
TLDR
The results showed a global reduction in miRNA expression levels in CLL cells associated to a consistent underexpression of miR-181a, let-7a andmiR-30d, and five novel miRNA candidates cloned from leukemic cells are reported.Abstract:
MicroRNAs (miRNAs) are a novel class of small noncoding RNA molecules that regulate gene expression by inducing degradation or translational inhibition of target mRNAs. There are more than 500 miRNA genes reported in the human genome, constituting one of the largest classes of regulatory genes. Increasing experimental evidence supports the idea of aberrant miRNA expression in cancer pathogenesis. We analyzed the pattern of miRNA expression in chronic lymphocytic leukemia (CLL) cells and our results showed a global reduction in miRNA expression levels in CLL cells associated to a consistent underexpression of miR-181a, let-7a and miR-30d. We observed overexpression of miR-155 and a set of five miRNAs that are differentially expressed between patients with different clinical outcomes. Five novel miRNA candidates cloned from leukemic cells are reported. Surprisingly, predicted mRNA targets for these novel miRNA revealed a high proportion of targets located in a small region of chromosome 1, which is frequently altered in human cancer. Additionally, several targets were shared by at least two of miRNA candidates. Predicted targets included several genes recently described as tumor suppressors. These data could afford new avenues for exploring innovative pathways in CLL biology and therapy.read more
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Modulation of microRNA processing by p53
Hiroshi I. Suzuki,Kaoru Yamagata,Koichi Sugimoto,Takashi Iwamoto,Shigeaki Kato,Kohei Miyazono +5 more
TL;DR: This study reveals a previously unrecognized function of p53 in miRNA processing, which may underlie key aspects of cancer biology, and suggests that transcription-independent modulation of miRNA biogenesis is intrinsically embedded in a tumour suppressive program governed by p53.
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miR-155 gene: a typical multifunctional microRNA.
TL;DR: The available experimental evidence indicating that miR-155 is over expressed in a variety of malignant tumors allows us to include this miRNA in the list of genes of paramount importance in cancer diagnosis and prognosis.
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MicroRNAs: key players in the immune system, differentiation, tumorigenesis and cell death
TL;DR: The role of miRNAs and their targets in contributing to human cancers and their function as regulators of apoptotic pathways and the immune system are summarized.
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MicroRNAs: Novel Biomarkers for Human Cancer
TL;DR: The biologic roles of miRNAs in cancer suggest a correlation with prognosis and therapeutic outcome, and may lead to new approaches for the categorization, diagnosis, and treatment of human cancers.
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MicroRNA-155 Suppresses Activation-Induced Cytidine Deaminase-Mediated Myc-Igh Translocation
Yair Dorsett,Kevin M. McBride,Mila Jankovic,Anna Gazumyan,Anna Gazumyan,To-Ha Thai,Davide F. Robbiani,Michela Di Virgilio,Bernardo Reina San-Martin,Gordon F. Heidkamp,Tanja A. Schwickert,Thomas Eisenreich,Klaus Rajewsky,Michel C. Nussenzweig,Michel C. Nussenzweig +14 more
TL;DR: It is indicated that miR-155 can act as a tumor suppressor by reducing potentially oncogenic translocations generated by AID.
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