Structure-Based Design of a Fusion Glycoprotein Vaccine for Respiratory Syncytial Virus
Jason S. McLellan,Man Chen,M. Gordon Joyce,Mallika Sastry,Guillaume Stewart-Jones,Yongping Yang,Baoshan Zhang,Lei Chen,Sanjay Srivatsan,Anqi Zheng,Tongqing Zhou,Kevin W. Graepel,Azad Kumar,Syed M. Moin,Jeffrey C. Boyington,Gwo-Yu Chuang,Cinque Soto,Ulrich Baxa,Arjen Q. Bakker,Hergen Spits,Tim Beaumont,Zizheng Zheng,Ningshao Xia,Sung Youl Ko,John Paul Todd,Srinivas S. Rao,Barney S. Graham,Peter D. Kwong +27 more
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TLDR
A viral antigen is sought that provides greater protection than currently available vaccines and focused on antigenic site Ø, a metastable site specific to the prefusion state of the RSV fusion (F) glycoprotein, as this site is targeted by extremely potent RSV-neutralizing antibodies.Abstract:
Respiratory syncytial virus (RSV) is the leading cause of hospitalization for children under 5 years of age. We sought to engineer a viral antigen that provides greater protection than currently available vaccines and focused on antigenic site O, a metastable site specific to the prefusion state of the RSV fusion (F) glycoprotein, as this site is targeted by extremely potent RSV-neutralizing antibodies. Structure-based design yielded stabilized versions of RSV F that maintained antigenic site O when exposed to extremes of pH, osmolality, and temperature. Six RSV F crystal structures provided atomic-level data on how introduced cysteine residues and filled hydrophobic cavities improved stability. Immunization with site O–stabilized variants of RSV F in mice and macaques elicited levels of RSV-specific neutralizing activity many times the protective threshold.read more
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Fusing safety and efficacy: A maternal bivalent RSV prefusion F vaccine
TL;DR: Kampmann et al. as discussed by the authors demonstrated safety and efficacy of a maternal bivalent RSV prefusion F vaccine in reducing severe RSV-associated lower respiratory tract infections in infants at 90 and 180 days following birth.
Pöördvaktsinoloogia ja struktuuribioloogia: uued võtted vaktsiiniarenduses
TL;DR: Klassikalised pohimotted vaktsinoloogias formuleeris 19. sajandi lopul L. Pasteur as mentioned in this paper, see on andnud meile vaktiniine 27 haigustekitaja vastu ning viinud paljude nakkushaiguste leviku olulise vahenemiseni.
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Promising New Approach to Vaccine for Respiratory Syncytial Virus
TL;DR: Respiratory syncytial virus (RSV) is an important cause of death in neonates and elders and more than 3 million young children are hospitalized globally.
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Systematic conformation-to-phenotype mapping via limited deep sequencing of proteins.
Eugene Serebryany,Victor Zhao,Ki Bum Park,Amir Bitran,Sunia A. Trauger,Bogdan Budnik,Eugene I. Shakhnovich +6 more
TL;DR: In this paper , the authors carried out double-Cys mutational scanning of E. coli periplasmic chaperone HdeA to identify disordered hydrophobic conformers with variable cytotoxicity.
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TL;DR: The Global Burden of Diseases, Injuries, and Risk Factors Study 2010 aimed to estimate annual deaths for the world and 21 regions between 1980 and 2010 for 235 causes, with uncertainty intervals (UIs), separately by age and sex, using the Cause of Death Ensemble model.
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