Test sensitivity is secondary to frequency and turnaround time for COVID-19 screening.
Daniel B. Larremore,Bryan Wilder,Evan Lester,Evan Lester,Soraya Shehata,Soraya Shehata,James M. Burke,James A. Hay,Milind Tambe,Michael J. Mina,Michael J. Mina,Roy Parker +11 more
TLDR
It is demonstrated that effective screening depends largely on frequency of testing and speed of reporting and is only marginally improved by high test sensitivity, and should prioritize accessibility, frequency, and sample-to-answer time.Abstract:
The COVID-19 pandemic has created a public health crisis. Because SARS-CoV-2 can spread from individuals with presymptomatic, symptomatic, and asymptomatic infections, the reopening of societies and the control of virus spread will be facilitated by robust population screening, for which virus testing will often be central. After infection, individuals undergo a period of incubation during which viral titers are too low to detect, followed by exponential viral growth, leading to peak viral load and infectiousness and ending with declining titers and clearance. Given the pattern of viral load kinetics, we model the effectiveness of repeated population screening considering test sensitivities, frequency, and sample-to-answer reporting time. These results demonstrate that effective screening depends largely on frequency of testing and speed of reporting and is only marginally improved by high test sensitivity. We therefore conclude that screening should prioritize accessibility, frequency, and sample-to-answer time; analytical limits of detection should be secondary.read more
Citations
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A lyophilized colorimetric RT-LAMP test kit for rapid, low-cost, at-home molecular testing of SARS-CoV-2 and other pathogens
Xinyu Song,Felicity Coulter,Ming Yang,Jessica L. Smith,Fikadu G. Tafesse,William B. Messer,John H. Reif +6 more
TL;DR: In this paper , a simple-to-use nucleic acid test kit for self-administered at-home testing without lab instrumentation is presented, which takes < 60 min, including noninvasive sample collection, one-step RNA preparation, reverse-transcription loop-mediated isothermal amplification in a thermos, and direct visual inspection of a colorimetric test result.
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Direct detection of SARS-CoV-2 RNA using high-contrast pH-sensitive dyes
Timothy A. Brown,Kathy Schaefer,Arthur Tsang,Hyun Ah Yi,Jonathan B. Grimm,Andrew L. Lemire,Fadi M. Jradi,Charles Kim,Kevin McGowan,Kimberly D. Ritola,Derek T. Armstrong,Heba H. Mostafa,Wyatt Korff,Ronald D. Vale,Luke D. Lavis +14 more
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A versatile CRISPR Cas12a-based point-of-care biosensor enabling convenient glucometer readout for ultrasensitive detection of pathogen nucleic acids.
TL;DR: Wang et al. as discussed by the authors proposed a point-of-care (POC) method for detection of pathogen nucleic acids by combining the clustered regularly interspaced short palindromic repeat (CRISPR) Cas12a-based assay and personal glucometer readout.
Evaluation of the Practicability of Biosynex Antigen Self-Test COVID-19 AG+ for the Detection of SARS-CoV-2 Nucleocapsid Protein from Self-Collected Nasal Mid-Turbinate Secretions in the General Public in France
Serge Tonen-Wolyec,Raphaël Dupont,Natalio Awaida,Salomon Batina-Agasa,Marie-Pierre Hayette,Laurent Bélec +5 more
TL;DR: In this paper, the authors evaluated the applicability of the Ag-RDST BIOSYNEX Antigen Self-Test COVID-19 Ag+ (Biosynex Swiss SA, Freiburg, Switzerland), using self-collected nasal secretions from the turbinate medium (NMT), in an unselected adult population living in France.
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Dogs Detecting COVID-19 From Sweat and Saliva of Positive People: A Field Experience in Mexico
J. M. Mancilla-Tapia,Victoria Lozano-Esparza,A. Orduna,R. F. Osuna-Chávez,Ramón Enrique Robles-Zepeda,Blayra Maldonado-Cabrera,Jorge Rubén Béjar-Cornejo,Iván Ruiz-León,Carlos Gabriel González-Becuar,Anna Hielm-Björkman,Ana Novelo-González,Víctor M. Vidal-Martínez +11 more
TL;DR: The performance of the dogs is considered promising because it is reasonable to expect that with gauze exposed for a longer time to sweat and saliva of people with COVID-19, their detection capacity would improve, and dogs could become important allies for the control of the CO VID-19 pandemic, especially in developing countries.
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