The GET Complex Mediates Insertion of Tail-Anchored Proteins into the ER Membrane
Maya Schuldiner,Jutta Metz,Volker Schmid,Vladimir Denic,Magdalena Rakwalska,Hans Dieter Schmitt,Blanche Schwappach,Jonathan S. Weissman +7 more
TLDR
It is shown that Get3, the yeast homolog of the TA-interacting factor Asna1/Trc40, specifically recognizes TMDs of TA proteins destined for the secretory pathway, which represents a key decision step, whose loss can lead to misinsertion ofTA proteins into mitochondria.About:
This article is published in Cell.The article was published on 2008-08-22 and is currently open access. It has received 468 citations till now. The article focuses on the topics: Membrane protein & Peripheral membrane protein.read more
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Comprehensive characterization of genes required for protein folding in the endoplasmic reticulum
Martin C. Jonikas,Sean R. Collins,Vladimir Denic,Eugene Oh,Erin M. Quan,Volker Schmid,Jimena Weibezahn,Blanche Schwappach,Peter Walter,Jonathan S. Weissman,Maya Schuldiner +10 more
TL;DR: This strategy revealed multiple conserved factors critical for endoplasmic reticulum folding, including an intimate dependence on the later secretory pathway, a previously uncharacterized six-protein transmembrane complex, and a co-chaperone complex that delivers tail-anchored proteins to their membrane insertion machinery.
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Interactions Between Nuclei and the Cytoskeleton Are Mediated by SUN-KASH Nuclear-Envelope Bridges
TL;DR: SUN and KASH proteins were identified through genetic screens for mispositioned nuclei in model organisms and have been linked to human diseases including muscular dystrophy, ataxia, progeria, lissencephaly, and cancer.
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Peroxisomes take shape
TL;DR: Ongoing studies are deciphering the diverse molecular mechanisms that underlie biogenesis and how they cooperate to dynamically control peroxisome utility to lead to an understanding of peroxISome dynamics that can be capitalized upon for bioengineering and the development of therapies to improve human health.
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Molecular mechanisms and physiological functions of mitophagy.
TL;DR: In this article, the authors review the current molecular understanding of mitophagy, and its physiological implications, and discuss how multiple mitophathy pathways coordinately modulate mitochondrial fitness and populations.
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Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling
TL;DR: A flexible deep sequencing–based methodology that enables precise characterization of localized protein synthesis in intact cells and suggests a role for polysomes in retaining mRNAs at the ER, allowing for efficient targeting of RNCs for translocation is applied.
References
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Mark S. Longtine,Amos Mckenzie,Douglas J. Demarini,Nirav Shah,Achim Wach,Arndt Brachat,Peter Philippsen,John R. Pringle +7 more
TL;DR: A new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications that should further facilitate the rapid analysis of gene function in S. cerevisiae.
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Global analysis of protein localization in budding yeast
Won-Ki Huh,James V. Falvo,Luke C. Gerke,Adam S. Carroll,Russell W. Howson,Jonathan S. Weissman,Erin K. O'Shea +6 more
TL;DR: The construction and analysis of a collection of yeast strains expressing full-length, chromosomally tagged green fluorescent protein fusion proteins helps reveal the logic of transcriptional co-regulation, and provides a comprehensive view of interactions within and between organelles in eukaryotic cells.
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Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry
Yuen Ho,Albrecht Gruhler,Adrian Heilbut,Gary D. Bader,Gary D. Bader,Lynda Moore,Sally-Lin Adams,Anna Millar,Paul J. Taylor,Keiryn L. Bennett,Kelly Boutilier,Lingyun Yang,Cheryl Wolting,Ian Donaldson,Søren Schandorff,Juanita Shewnarane,Mai Vo,Joanne Taggart,Marilyn Goudreault,Brenda Muskat,Cris Alfarano,Danielle Dewar,Zhen Lin,Katerina Michalickova,Katerina Michalickova,Andrew Willems,Andrew Willems,Holly Sassi,Peter A Nielsen,Karina Juhl Rasmussen,Jens R. Andersen,Lene E. Johansen,Lykke Haastrup Hansen,Hans Jespersen,Alexandre V. Podtelejnikov,Eva Nielsen,Janne S. Crawford,Vibeke Poulsen,Birgitte D Sørensen,Jesper Matthiesen,Ronald C. Hendrickson,Frank Gleeson,Tony Pawson,Tony Pawson,Michael Moran,Daniel Durocher,Daniel Durocher,Matthias Mann,Christopher W. V. Hogue,Christopher W. V. Hogue,Daniel Figeys,Mike Tyers,Mike Tyers +52 more
TL;DR: Comparison of the HMS-PCI data set with interactions reported in the literature revealed an average threefold higher success rate in detection of known complexes compared with large-scale two-hybrid studies.
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Genomic Libraries and a Host Strain Designed for Highly Efficient Two-Hybrid Selection in Yeast
TL;DR: A novel multienzyme approach was used to generate a set of highly representative genomic libraries from S. cerevisiae and a unique host strain was created that contains three easily assayed reporter genes, each under the control of a different inducible promoter.