Ultrastructural characterization of cystic fibrosis sputum using atomic force and scanning electron microscopy.
Reinhard Manzenreiter,Ferry Kienberger,Veronica Marcos,Kurt Schilcher,Wolf Dietrich Krautgartner,Astrid Obermayer,Marlene Huml,Walter Stoiber,Andreas Hector,Matthias Griese,Matthias Hannig,Michael Studnicka,Ljubomir Vitkov,Ljubomir Vitkov,Dominik Hartl +14 more
TLDR
It is suggested that excessive NET formation represents the major factor underlying the gel-like structure of CF sputum and provide evidence that CF-NETs contain ectosome-like structures that could represent targets for future therapeutic approaches.About:
This article is published in Journal of Cystic Fibrosis.The article was published on 2012-03-01 and is currently open access. It has received 189 citations till now. The article focuses on the topics: Sputum Cytology & Sputum.read more
Citations
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Targeting potential drivers of COVID-19: Neutrophil extracellular traps.
Betsy J. Barnes,José M. Adrover,Amelia Baxter-Stoltzfus,Alain C. Borczuk,Jonathan Cools-Lartigue,James M. Crawford,Juliane Daßler-Plenker,Philippe Guerci,Caroline Huynh,Jason S. Knight,Massimo Loda,Mark R. Looney,Florencia McAllister,Roni F. Rayes,Stéphane Renaud,Simon Rousseau,Steven P. Salvatore,Robert E. Schwartz,Jonathan Spicer,Christian C. Yost,Andrew G. Weber,Yu Zuo,Mikala Egeblad +22 more
TL;DR: Autopsy results and literature are presented supporting the hypothesis that neutrophil extracellular traps (NETs) may contribute to organ damage and mortality in COVID-19, and existing drugs that target NETs, although unspecific, may benefit CO VID-19 patients.
Journal ArticleDOI
Targeting microbial biofilms: current and prospective therapeutic strategies.
TL;DR: This Review focuses on current therapeutic strategies and those under development that target vital structural and functional traits of microbial biofilms and drug tolerance mechanisms, including the extracellular matrix and dormant cells.
Journal ArticleDOI
Neutrophil Extracellular Traps Directly Induce Epithelial and Endothelial Cell Death: A Predominant Role of Histones
Mona Saffarzadeh,Christiane Juenemann,Markus A. Queisser,Markus A. Queisser,Guenter Lochnit,Guillermo Barreto,Sebastian P. Galuska,Juergen Lohmeyer,Klaus T. Preissner +8 more
TL;DR: Data reveal the important role of protein components in NET, particularly histones, which may lead to host cell cytotoxicity and may be involved in lung tissue destruction.
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Modulating Innate and Adaptive Immunity by (R)-Roscovitine: Potential Therapeutic Opportunity in Cystic Fibrosis
Laurent Meijer,Deborah J. Nelson,Vladimir Riazanski,Aida G. Gabdoulkhakova,Geneviève Héry-Arnaud,Rozenn Le Berre,Nadège Loaëc,Nassima Oumata,Hervé Galons,Emmanuel Nowak,L. Guéganton,Guillaume Dorothée,Michaela Prochazkova,Bradford Hall,Ashok B. Kulkarni,Robert D. Gray,Adriano G. Rossi,Véronique Witko-Sarsat,Caroline Norez,Frédéric Becq,Denis Ravel,Dominique Mottier,Gilles Rault +22 more
TL;DR: The fact that (R)-roscovitine has undergone extensive preclinical safety/pharmacology studies, and phase I and II clinical trials in cancer patients, encourages its repurposing as a CF drug candidate.
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Neutrophil extracellular traps: Is immunity the second function of chromatin?
TL;DR: The understanding of how NETs are made, their function in infections and as danger signals, and their emerging importance in autoimmunity and coagulation are reviewed.
References
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Neutrophil extracellular traps kill bacteria
Volker Brinkmann,Ulrike Reichard,Christian Goosmann,Beatrix Fauler,Yvonne Uhlemann,David S. Weiss,Yvette Weinrauch,Yvette Weinrauch,Arturo Zychlinsky +8 more
TL;DR: It is described that, upon activation, neutrophils release granule proteins and chromatin that together form extracellular fibers that bind Gram-positive and -negative bacteria, which degrade virulence factors and kill bacteria.
Journal ArticleDOI
Neutrophils and immunity: challenges and opportunities.
TL;DR: Neutrophils inform and shape immune responses, contribute to the repair of tissue as well as its breakdown, use killing mechanisms that enrich the concepts of specificity, and offer exciting opportunities for the treatment of neoplastic, autoinflammatory and autoimmune disorders.
Journal ArticleDOI
Novel cell death program leads to neutrophil extracellular traps
Tobias A. Fuchs,Ulrike Abu Abed,Christian Goosmann,Robert Hurwitz,Ilka Schulze,Volker Wahn,Yvette Weinrauch,Volker Brinkmann,Arturo Zychlinsky +8 more
TL;DR: This novel ROS-dependent death allows neutrophils to fulfill their antimicrobial function, even beyond their lifespan.
Journal ArticleDOI
Netting neutrophils in autoimmune small-vessel vasculitis.
Kai Kessenbrock,Markus Krumbholz,Ulf Schönermarck,Walter Back,Wolfgang Gross,Zena Werb,Hermann-Josef Gröne,Volker Brinkmann,Dieter E. Jenne +8 more
TL;DR: It is shown that chromatin fibers, so-called neutrophil extracellular traps (NETs), are released by ANCA-stimulated neutrophils and contain the targeted autoantigens proteinase-3 and myeloperoxidase (MPO).
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Neutrophil Extracellular Traps Contain Calprotectin, a Cytosolic Protein Complex Involved in Host Defense against Candida albicans
Constantin F. Urban,David Ermert,Monika Schmid,Ulrike Abu-Abed,Christian Goosmann,Wolfgang Nacken,Volker Brinkmann,Peter R. Jungblut,Arturo Zychlinsky +8 more
TL;DR: The present investigations confirmed the antifungal activity of calprotectin in vitro and demonstrated that it contributes to effective host defense against C. albicans in vivo.