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What is next generation sequencing

TLDR
The aim of this article is to review the potential applications of NGS in paediatrics and to assess the need for further research into this area.
Abstract
Next generation sequencing (NGS), massively parallel or deep sequencing are related terms that describe a DNA sequencing technology which has revolutionised genomic research. Using NGS an entire human genome can be sequenced within a single day. In contrast, the previous Sanger sequencing technology, used to decipher the human genome, required over a decade to deliver the final draft. Although in genome research NGS has mostly superseded conventional Sanger sequencing, it has not yet translated into routine clinical practice. The aim of this article is to review the potential applications of NGS in paediatrics.

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Exosomal miRNAs as cancer biomarkers and therapeutic targets

TL;DR: If ex-miRNA is to be employed in novel non-invasive diagnostic approaches and as a therapeutic target in cancer, two further advances are necessary: in methods to isolate and detect ex- miRNA, and a better understanding of their biogenesis and functions in tumour-cell communication.
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Precision Medicine and Radiogenomics in Breast Cancer: New Approaches toward Diagnosis and Treatment

TL;DR: Radiogenomics may provide voxel-by-voxel genetic information for a complete, heterogeneous tumor or, in the setting of metastatic disease, set of tumors and thereby guide tailored therapy, thereby allowing for more precise imaging and screening.
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Evaluating measurable residual disease in acute myeloid leukemia.

TL;DR: Inclusion of MRD as an end point in future randomized clinical trials will provide the data needed to move toward standardizing MRD assays and may provide a more accurate assessment of therapeutic efficacy than current morphologic measures.
Journal ArticleDOI

Review of Recent DNA-Based Methods for Main Food-Authentication Topics.

TL;DR: The improved performance of most recent DNA-based tools in term of sensitivity, multiplexing ability, high-throughput, and relatively low-cost give them a game-changing role in food-authenticity-related topics.
Journal ArticleDOI

Can next generation sequencing play a role in detecting pathogens in synovial fluid

TL;DR: While next‐generation sequencing detected several organisms in each sample, in most samples with a higher probability of infection, there was a predominant organism present, while in those presumed not to be infected, many organisms were identified with no predominant organism.
References
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Journal ArticleDOI

Whole-genome sequencing for analysis of an outbreak of meticillin-resistant Staphylococcus aureus: a descriptive study

TL;DR: W whole-genome sequencing data were used to propose and confirm that MRSA carriage by a staff member had allowed the outbreak to persist during periods without known infection on the SCBU and after a deep clean.
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Trending Questions (1)
What is Next Generation Sequencing?

Next Generation Sequencing (NGS) is a DNA sequencing technology that allows for the rapid sequencing of entire genomes, revolutionizing genomic research.