What is next generation sequencing
Sam Behjati,Patrick S. Tarpey +1 more
TLDR
The aim of this article is to review the potential applications of NGS in paediatrics and to assess the need for further research into this area.Abstract:
Next generation sequencing (NGS), massively parallel or deep sequencing are related terms that describe a DNA sequencing technology which has revolutionised genomic research. Using NGS an entire human genome can be sequenced within a single day. In contrast, the previous Sanger sequencing technology, used to decipher the human genome, required over a decade to deliver the final draft. Although in genome research NGS has mostly superseded conventional Sanger sequencing, it has not yet translated into routine clinical practice. The aim of this article is to review the potential applications of NGS in paediatrics.read more
Citations
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Exosomal miRNAs as cancer biomarkers and therapeutic targets
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Review of Recent DNA-Based Methods for Main Food-Authentication Topics.
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Can next generation sequencing play a role in detecting pathogens in synovial fluid
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References
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Journal ArticleDOI
Analysis of Circulating Tumor DNA to Monitor Metastatic Breast Cancer
Sarah-Jane Dawson,Dana W.Y. Tsui,Muhammed Murtaza,Heather Biggs,Oscar M. Rueda,Suet-Feung Chin,Mark J Dunning,Davina Gale,Tim Forshew,Betania Mahler-Araujo,Sabrina Rajan,Sean Humphray,Jennifer Becq,David Halsall,Matthew G. Wallis,David Bentley,Carlos Caldas,Nitzan Rosenfeld +17 more
TL;DR: This proof-of-concept analysis showed that circulating tumor DNA is an informative, inherently specific, and highly sensitive biomarker of metastatic breast cancer.
Journal ArticleDOI
Noninvasive prenatal diagnosis of fetal chromosomal aneuploidy by massively parallel genomic sequencing of DNA in maternal plasma.
Rossa W.K. Chiu,K.C. Allen Chan,Yuan Gao,Virginia Y. M. Lau,Wenli Zheng,Tak Yeung Leung,Chris H. F. Foo,Bin Xie,Nancy B.Y. Tsui,Fiona M.F. Lun,Benny Zee,Tze K. Lau,Charles R. Cantor,Y.M. Dennis Lo +13 more
TL;DR: Massively parallel plasma DNA sequencing represents a new approach that is potentially applicable to all pregnancies for the noninvasive prenatal diagnosis of fetal chromosomal aneuploidies.
Journal ArticleDOI
Whole-genome sequencing for analysis of an outbreak of meticillin-resistant Staphylococcus aureus: a descriptive study
Simon R. Harris,Edward J. P. Cartwright,M. Estée Török,Matthew T. G. Holden,Nicholas M. Brown,Amanda Ogilvy-Stuart,Matthew J. Ellington,Michael A. Quail,Stephen D. Bentley,Julian Parkhill,Sharon J. Peacock +10 more
TL;DR: W whole-genome sequencing data were used to propose and confirm that MRSA carriage by a staff member had allowed the outbreak to persist during periods without known infection on the SCBU and after a deep clean.
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