Showing papers on "Catalase published in 2019"
TL;DR: H2 O2 oxidizes specific cysteine residues of target proteins to the sulfenic acid form and, similar to other organisms, this modification could initiate thiol-based redox relays and modify target enzymes, receptor kinases and transcription factors.
Abstract: Contents Summary 1197 I Introduction 1198 II Measurement and imaging of H2 O2 1198 III H2 O2 and O2 ·- toxicity 1199 IV Production of H2 O2 : enzymes and subcellular locations 1200 V H2 O2 transport 1205 VI Control of H2 O2 concentration: how and where? 1205 VII Metabolic functions of H2 O2 1207 VIII H2 O2 signalling 1207 IX Where next? 1209 Acknowledgements 1209 References 1209 SUMMARY: Hydrogen peroxide (H2 O2 ) is produced, via superoxide and superoxide dismutase, by electron transport in chloroplasts and mitochondria, plasma membrane NADPH oxidases, peroxisomal oxidases, type III peroxidases and other apoplastic oxidases Intracellular transport is facilitated by aquaporins and H2 O2 is removed by catalase, peroxiredoxin, glutathione peroxidase-like enzymes and ascorbate peroxidase, all of which have cell compartment-specific isoforms Apoplastic H2 O2 influences cell expansion, development and defence by its involvement in type III peroxidase-mediated polymer cross-linking, lignification and, possibly, cell expansion via H2 O2 -derived hydroxyl radicals Excess H2 O2 triggers chloroplast and peroxisome autophagy and programmed cell death The role of H2 O2 in signalling, for example during acclimation to stress and pathogen defence, has received much attention, but the signal transduction mechanisms are poorly defined H2 O2 oxidizes specific cysteine residues of target proteins to the sulfenic acid form and, similar to other organisms, this modification could initiate thiol-based redox relays and modify target enzymes, receptor kinases and transcription factors Quantification of the sources and sinks of H2 O2 is being improved by the spatial and temporal resolution of genetically encoded H2 O2 sensors, such as HyPer and roGFP2-Orp1 These H2 O2 sensors, combined with the detection of specific proteins modified by H2 O2 , will allow a deeper understanding of its signalling roles
478 citations
TL;DR: The direct and indirect involvement of deficiency and/or modification of catalase in the pathogenesis of some important diseases such as diabetes mellitus, Alzheimer's disease, Parkinson’s disease, vitiligo, and acatalasemia is described.
Abstract: Reactive species produced in the cell during normal cellular metabolism can chemically react with cellular biomolecules such as nucleic acids, proteins, and lipids, thereby causing their oxidative modifications leading to alterations in their compositions and potential damage to their cellular activities. Fortunately, cells have evolved several antioxidant defense mechanisms (as metabolites, vitamins, and enzymes) to neutralize or mitigate the harmful effect of reactive species and/or their byproducts. Any perturbation in the balance in the level of antioxidants and the reactive species results in a physiological condition called "oxidative stress." A catalase is one of the crucial antioxidant enzymes that mitigates oxidative stress to a considerable extent by destroying cellular hydrogen peroxide to produce water and oxygen. Deficiency or malfunction of catalase is postulated to be related to the pathogenesis of many age-associated degenerative diseases like diabetes mellitus, hypertension, anemia, vitiligo, Alzheimer's disease, Parkinson's disease, bipolar disorder, cancer, and schizophrenia. Therefore, efforts are being undertaken in many laboratories to explore its use as a potential drug for the treatment of such diseases. This paper describes the direct and indirect involvement of deficiency and/or modification of catalase in the pathogenesis of some important diseases such as diabetes mellitus, Alzheimer's disease, Parkinson's disease, vitiligo, and acatalasemia. Details on the efforts exploring the potential treatment of these diseases using a catalase as a protein therapeutic agent have also been described.
372 citations
TL;DR: The novel findings of cellular processes induced by ROS and RNS are reviewed and the functions of cellular endogenous antioxidant systems as well as natural anti-oxidative compounds in plants are recapitulate to facilitate the illustration of the imperative role of antioxidants in prevention against oxidative stress.
233 citations
TL;DR: Results evidently showed that MT enhanced tolerance of wheat seedlings to Cd toxicity by triggering the endogenous NO, and both MT and NO together enhanced Cd tolerance in wheat.
211 citations
TL;DR: In this article, the authors describe the various types of reactive oxygen species (ROS) and their production, and antioxidant defense mechanisms for ROS suppression, and describe the different types of ROS and their defense mechanisms.
Abstract: Reactive oxygen species (ROS) typically produce in algae and act as secondary messengers in numerous cellular processes. Under abiotic stresses, the balance between production and suppression of ROS disappears and causes increase of ROS. Increasing excessive ROS can cause damage to various cellular components comprising cell membranes, proteins and lipids. Algae have an antioxidant defense system to overcome on oxidative damage. Antioxidant defense mechanisms are of two types, namely enzymatic and non-enzymatic antioxidants. The enzymatic antioxidants include superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase. The non-enzymatic antioxidants include carotenoids, tocopherol, ascorbic acid, glutathione, flavonoids and phenolic compounds. In this review, we describe the various types of ROS and their production, and antioxidant defense mechanisms for ROS suppression.
180 citations
TL;DR: Atomically dispersed Fe-N4 sites anchored on N-doped porous carbon materials can mimic two antioxidative enzymes of catalase and superoxide dismutase and serve as a bifunctional single-atom-based enzyme (SAzyme) for scavenging reactive oxygen species (ROS) to remove excess ROS generated during oxidative stress in cells.
171 citations
TL;DR: It is found that low-dose curcumin protected cells from apoptosis, while apoptosis in the middle- and high-doseCurcumin-treated groups were stagnant in the early stage, which could potentially promote cell survival.
Abstract: Large-scale breeding environments often lead to oxidative stress. Macrophages play an important role in the immune system and are vulnerable to reactive oxygen species (ROS), which result in macrophage death. Curcumin is the main active component of turmeric and exerts antioxidant effects. Here, we measured the activity of some antioxidant enzymes and chose the Nrf2-Keap1 signaling pathway to study the protective effects of curcumin on macrophages under oxidative stress in vitro. We used RAW264.7 cells as a research model, and oxidative damage was induced by hydrogen peroxide (H2O2). Cell viability was measured by an MTT assay. Flow cytometry was used to measure cellular ROS and apoptosis. The effect of curcumin on Nrf2-Keap1 signaling pathway-related genes was analyzed by qRT-PCR. Furthermore, the translocation of Nrf2 protein was also investigated by Western blot analysis of total and nuclear proteins. All curcumin-treated groups exhibited increased activity of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX). Low- and middle-dose curcumin decreased malondialdehyde (MDA) and ROS levels, but high-dose curcumin increased MDA and ROS production. We found that low-dose curcumin protected cells from apoptosis, while apoptosis in the middle- and high-dose curcumin-treated groups were stagnant in the early stage. Furthermore, middle-dose curcumin upregulated Nrf2 expression after H2O2 treatment for 4 h. Low- and middle-dose curcumin could activate Nrf2 and promote it to migrate into nuclei. The translocation of Nrf2 to the nucleus to upregulate the expression of haemoxygenase-1 (HO-1) was promoted in the low- and middle-dose curcumin-treated groups. The middle-dose curcumin-treated group also exhibited enhanced expression of glutamate-cysteine ligase, a modifier subunit (GLCM), but inhibited transcription of glutamate-cysteine ligase, a catalytic subunit (GCLC). Curcumin resisted oxidants by increasing the activity of antioxidant enzymes and activating the Nrf2-Keap1 pathway, which could potentially promote cell survival.
137 citations
TL;DR: It is suggested that melatonin has beneficial effects on tomato seedlings growth under both stress and non-stress conditions and its role in tolerance to salt stress may be associated with the regulation of enzymes involved in photosynthesis, the antioxidant system, metabolism of proline and carbohydrate, and the ASC—GSH cycle.
Abstract: Melatonin, a natural agent, has multiple functions in animals as well as in plants. However, its possible roles in plants under abiotic stress are not clear. Nowadays, soil salinity is a major threat to global agriculture because a high soil salt content causes multiple stresses (hyperosmotic, ionic, and oxidative). Therefore, the aim of the present study was to explore: (1) the involvement of melatonin in biosynthesis of photosynthetic pigments and in regulation of photosynthetic enzymes, such as carbonic anhydrase (CA) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco); (2) the role of melatonin in osmoregulation by proline and carbohydrate metabolism; and (3) the function of melatonin in the antioxidant defense system under salinity. Outcomes of the study reveal that under non-saline conditions, application of melatonin (20 and 50 µM) improved plant growth, viz. shoot length, root length, shoot fresh weight (FW), root FW, shoot dry weight (DW), root DW and leaf area and physio-biochemical parameters [chlorophyll (Chl) a and b, proline (Pro) and total soluble carbohydrates (TSC) content, and increased the activity of CA and Rubisco]. However, tomato seedlings treated with NaCl exhibited enhanced Chl degradation, electrolyte leakage (EL), malondialdehyde (MDA) and reactive oxygen species (ROS; superoxide and hydrogen peroxide). ROS were detected in leaf and root. Interestingly, application of melatonin improved plant growth and reduced EL, MDA and ROS levels through upregulation of photosynthesis enzymes (CA, Rubisco), antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase and ascorbate reductase) and levels of non-enzymatic antioxidants [ascorbate (ASC) and reduced glutathione (GSH)], as well as by affecting the ASC—GSH cycle. Additionally, exogenous melatonin also improved osmoregulation by increasing the content of TSC, Pro and Δ1-pyrroline-5-carboxylate synthetase activity. These results suggest that melatonin has beneficial effects on tomato seedlings growth under both stress and non-stress conditions. Melatonin’s role in tolerance to salt stress may be associated with the regulation of enzymes involved in photosynthesis, the antioxidant system, metabolism of proline and carbohydrate, and the ASC—GSH cycle. Also, melatonin could be responsible for maintaining the high ratios of GSH/GSSG and ASC/DHA.
133 citations
TL;DR: It is suggested that p-CA is a potent antioxidant with potential therapeutic efficacy for treating hyperlipidemia symptoms and moderate antioxidant activity in the selected in vitro assay.
108 citations
TL;DR: This model explains the selectivity of CAP and PAM action towards tumor cells and is in contradiction to previous models that implicated that ROS/RNS from CAP or PAM were sufficient to directly cause cell death of tumor cells.
Abstract: Nitrite and H2O2 are long-lived species in cold atmospheric plasma and plasma-activated medium. It is known that their synergistic interaction is required for selective apoptosis induction in tumor cells that are treated with plasma-activated medium. This study shows that the interaction between nitrite and H2O2 leads to the formation of peroxynitrite, followed by singlet oxygen generation through the interaction between peroxynitrite and residual H2O2. This primary singlet oxygen causes local inactivation of few catalase molecules on the surface of tumor cells. As a consequence, H2O2 and peroxynitrite that are constantly produced by tumor cells and are usually decomposed by their protective membrane-associated catalase, are surviving at the site of locally inactivated catalase. This leads to the generation of secondary singlet oxygen through the interaction between tumor cell-derived H2O2 and peroxynitrite. This selfsustained process leads to autoamplification of secondary singlet oxygen generation and catalase inactivation. Inactivation of catalase allows the influx of H2O2 through aquaporins, leading to intracellular glutathione depletion and sensitization of the cells for apoptosis induction through lipid peroxidation. It also allows to establish intercellular apoptosis-inducing HOCl signaling, driven by active NOX1 and finalized by lipid peroxidation through hydroxyl radicals that activates the mitochondrial pathway of apoptosis. This experimentally established model is based on a triggering function of CAP and PAM-derived H2O2/nitrite that causes selective cell death in tumor cells based on their own ROS and RNS. This model explains the selectivity of CAP and PAM action towards tumor cells and is in contradiction to previous models that implicated that ROS/RNS from CAP or PAM were sufficient to directly cause cell death of tumor cells.
92 citations
TL;DR: Interestingly, exogenous SA treatment mitigated Cd toxicity by increasing the relative water content, chlorophyll, proline, and endogenous SA contents along with decline in malondialdehyde (MDA), hydrogen peroxide (H2O2), and superoxide anion radicals (O2-).
TL;DR: A key conclusion from these experiments is that tumor cell-generated RONS play the major role in inactivating protective catalase, depleting glutathione and establishing apoptosis-inducing RONS signaling.
Abstract: The selective in vitro anti-tumor mechanisms of cold atmospheric plasma (CAP) and plasma-activated media (PAM) follow a sequential multi-step process. The first step involves the formation of primary singlet oxygen (1O2) through the complex interaction between NO2− and H2O2. 1O2 then inactivates some membrane-associated catalase molecules on at least a few tumor cells. With some molecules of their protective catalase inactivated, these tumor cells allow locally surviving cell-derived, extracellular H2O2 and ONOO─ to form secondary 1O2. These species continue to inactivate catalase on the originally triggered cells and on adjacent cells. At the site of inactivated catalase, cell-generated H2O2 enters the cell via aquaporins, depletes glutathione and thus abrogates the cell’s protection towards lipid peroxidation. Optimal inactivation of catalase then allows efficient apoptosis induction through the HOCl signaling pathway that is finalized by lipid peroxidation. An identical CAP exposure did not result in apoptosis for nonmalignant cells. A key conclusion from these experiments is that tumor cell-generated RONS play the major role in inactivating protective catalase, depleting glutathione and establishing apoptosis-inducing RONS signaling. CAP or PAM exposure only trigger this response by initially inactivating a small percentage of protective membrane associated catalase molecules on tumor cells.
TL;DR: The current study suggests the promising application of SNPs as an innovative approach to mitigate Al phytotoxicity in acidic soils and provides a comprehensive view of the cellular and biochemical mechanisms underlying this mitigation capacity.
TL;DR: Evidence is provided of the presence of H2 S in plant peroxisomes which appears to regulate catalase activity and, consequently, theperoxisomal H2 O2 metabolism.
Abstract: Plant peroxisomes have the capacity to generate different reactive oxygen and nitrogen species (ROS and RNS), such as H2 O2 , superoxide radical (O2 · - ), nitric oxide and peroxynitrite (ONOO- ). These organelles have an active nitro-oxidative metabolism which can be exacerbated by adverse stress conditions. Hydrogen sulfide (H2 S) is a new signaling gasotransmitter which can mediate the posttranslational modification (PTM) persulfidation. We used Arabidopsis thaliana transgenic seedlings expressing cyan fluorescent protein (CFP) fused to a canonical peroxisome targeting signal 1 (PTS1) to visualize peroxisomes in living cells, as well as a specific fluorescent probe which showed that peroxisomes contain H2 S. H2 S was also detected in chloroplasts under glyphosate-induced oxidative stress conditions. Peroxisomal enzyme activities, including catalase, photorespiratory H2 O2 -generating glycolate oxidase (GOX) and hydroxypyruvate reductase (HPR), were assayed in vitro with a H2 S donor. In line with the persulfidation of this enzyme, catalase activity declined significantly in the presence of the H2 S donor. To corroborate the inhibitory effect of H2 S on catalase activity, we also assayed pure catalase from bovine liver and pepper fruit-enriched samples, in which catalase activity was inhibited. Taken together, these data provide evidence of the presence of H2 S in plant peroxisomes which appears to regulate catalase activity and, consequently, the peroxisomal H2 O2 metabolism.
TL;DR: Overall, H2O2 treatment improved growth, photosynthesis, metabolic state of the plants which provided tolerance and helped the plants to cope well under Cu stress.
TL;DR: It is concluded that the AgNPs can be effectively utilized for the enhancement of bioactive antioxidants in the callus cultures of C. tuberculata, a highly medicinal and threatened plant.
Abstract: Elicited plant in vitro cultures are gaining more interest worldwide for their potential in the uniform production of industrially important secondary metabolites. In the present study, different ratios of silver nanoparticles (AgNPs) and plant growth regulators (PGRs) were supplemented to in vitro cultures for the sustainable production of biomass and antioxidant secondary metabolites through callus cultures of Caralluma tuberculata. Results indicated that various concentrations of AgNPs significantly affected the callus proliferation and substantially increased the callus biomass, when combined with PGRs in the MS (Murashige and Skoog) media. The highest fresh (0.78 g/l) and dry (0.051 g/l) biomass accumulation of callus was observed in the cultures raised in vitro at 60 µg/l AgNPs in combination with 0.5 mg/l 2,4-D plus 3.0 mg/l BA. Phytochemical analysis of the callus cultures showed higher production of phenolics (TPC:3.0 mg), flavonoids (TFC:1.8 mg), phenylalanine ammonialyase activity (PAL: 5.8 U/mg) and antioxidant activity (90%), respectively, in the callus cultures established on MS media in the presence of 90 ug/l AgNPs. Moreover, enhanced activities of antioxidant enzymes such as superoxide dismutase (SOD: 4.8 U/mg), peroxidase (POD: 3.3 U/mg), catalase (CAT: 2.5 U/mg) and ascorbate peroxidase (APX: 1.9 U/mg) were detected at higher level (90 ug/l) of AgNPs tested alone for callus proliferation in the MS media. It may be concluded that the AgNPs can be effectively utilized for the enhancement of bioactive antioxidants in the callus cultures of C. tuberculata, a highly medicinal and threatened plant. This protocol can be scaled up for the industrial production of plant biomass and pharmacologically potent metabolites in C. tuberculata.
TL;DR: Overall, this study demonstrates the metabolic, neurotoxic and oxidative stress impacts of SA in M. galloprovincialis, which may result in negative consequences at the population level.
TL;DR: It is concluded that H2S induced oxidative stress and then leaded to excessive mitochondrial fission, which involved in apoptosis and damage broiler myocardia.
TL;DR: Investigation of the effects of drought stress on the activity of antioxidant enzymes and osmotic adjustment substance content in the tillering period of drought-sensitive and drought-tolerant rice cultivars showed that the superoxide dismutase, peroxidase, catalase activity, hydrogen peroxide content, soluble protein content and soluble sugar content increased with the accumulation of time and intensity of drought Stress.
Abstract: The objective of this study was to investigate the effects of drought stress on the activity of antioxidant enzymes and osmotic adjustment substance content in the tillering period of drought-sensitive and drought-tolerant rice cultivars. The results showed that the superoxide dismutase (SOD), peroxidase (POD), catalase activity (CAT), hydrogen peroxide content, soluble protein content and soluble sugar content increased with the accumulation of time and intensity of drought stress. Compared with the drought-sensitive cultivar, drought-resistant cultivar had a smaller photosynthetic affected area, longer CAT enzyme activity duration, and lower H2O2 accumulation. Unlike POD and CAT enzymes, which maintain the ability to scavenge hydrogen peroxide under long drought conditions, ascorbate peroxidase (APX) enzymes seem to be a rapid response mechanism to scavenge hydrogen peroxide under drought stress. Under a −10 kPa water potential, using soluble sugars on the osmotic adjustment ability of the drought-resistant cultivars was more efficient; under −40 kPa water potential, drought-resistant cultivars can maintain relative high levels of ascorbate (ASA) content in the short term. After the restoration of irrigation, the indices gradually returned to control levels. The ASA content showed faster accumulation ability in drought-resistant cultivars and faster recovery. The soluble protein content recovered more slowly in drought-sensitive cultivars under the −40 kPa treatment. Drought-resistant cultivars showed stronger resistance to drought in the −10 kPa treatment and obtained similar yield to the control, while the drought-sensitive cultivars were more obviously affected by the drought stress.
TL;DR: A review of reactions of catalases with their main substrate, hydrogen peroxide, and with oxidizing species such as hydroxyl radical, superoxide, nitric oxide, peroxynitrite, hypochlorous acid, and singlet oxygen is provided.
TL;DR: An important role of MT is demonstrated in the relief of salt stress and, therefore, this study provides a reference for managing salinity in naked oat.
Abstract: Melatonin (MT; N-acetyl-5-methoxytryptamine) is a pleiotropic signaling molecule that has been demonstrated to play an important role in plant growth, development, and regulation of environmental stress responses. Studies have been conducted on the role of the exogenous application of MT in a few species, but the potential mechanisms of MT-mediated stress tolerance under salt stress are still largely unknown. In this study, naked oat seedlings under salt stress (150 mM NaCl) were pretreated with two different concentrations of MT (50 and 100 μM), and the effects of MT on the growth and antioxidant capacity of naked oat seedlings were analyzed to explore the regulatory effect of MT on salt tolerance. The results showed that pretreating with different concentrations of MT promoted the growth of seedlings in response to 150 mM NaCl. Different concentrations of MT reduced hydrogen peroxide, superoxide anion, and malondialdehyde contents. The exogenous application of MT also increased superoxide dismutase, peroxidase, catalase, and ascorbate peroxide activities. Chlorophyll content, leaf area, leaf volume, and proline increased in the leaves of naked oat seedlings under 150 mM NaCl stress. MT upregulated the expression levels of the lipid peroxidase genes lipoxygenase and peroxygenase, a chlorophyll biosynthase gene (ChlG), the mitogen-activated protein kinase genes Asmap1 and Aspk11, and the transcription factor genes (except DREB2), NAC, WRKY1, WRKY3, and MYB in salt-exposed MT-pretreated seedlings when compared with seedlings exposed to salt stress alone. These results demonstrate an important role of MT in the relief of salt stress and, therefore, provide a reference for managing salinity in naked oat.
TL;DR: antioxidant enzymes and metabolites against reactive oxygen species (ROS) including polyphenol oxidase (PPO), peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), alcohol dehydrogenase (ADH), reduced glutathione (GSH), malondialdehyde (MDA), flavonoid and polyphenolic contents were also significantly relieved by inoculation of IU01 and IU02 in plant exposed to different concentration of
TL;DR: It is reported in this study that metallothionein and RACK1 play a vital role in the reactive oxygen species mediated signal transduction pathway to enhance the speed of germination in magnetoprimed tomato seeds.
Abstract: Seeds of tomato were magnetoprimed at 100 mT for 30 min followed by imbibition for 12 and 24 h, respectively, at 20 °C, to examine the biochemical and molecular changes involved in homeostasis of hydrogen peroxide (H2O2) and its signaling associated with hormone interactions for promoting vigor. The relative transcript profiles of genes involved in the synthesis of H2O2 like Cu-amine oxidase (AO), receptor for activated C kinase 1 (RACK1) homologue (ArcA2) and superoxide dismutase (SOD1 and SOD9) increased in magnetoprimed tomato seeds as compared to unprimed ones with a major contribution (21.7-fold) from Cu-amine oxidase. Amongst the genes involved in the scavenging of H2O2 i.e, metallothionein (MT1, MT3 and MT4), catalase (CAT1) and ascorbate peroxidase (APX1 and APX2), MT1 and MT4 exhibited 14.4- and 15.4-fold increase respectively, in the transcript abundance, in primed seeds compared to the control. We report in our study that metallothionein and RACK1 play a vital role in the reactive oxygen species mediated signal transduction pathway to enhance the speed of germination in magnetoprimed tomato seeds. Increased enzymatic activities of catalase and ascorbate peroxidase were observed at 12 h of imbibition in the magnetoprimed seeds indicating their roles in maintaining H2O2 levels in the primed seeds. The upregulation of ABA 8′-hydroxylase and GA3 oxidase1 genes eventually, lead to the decreased abscisic acid/gibberellic acid (ABA/GA3) ratio in the primed seeds, suggesting the key role of H2O2 in enhancing the germination capacity of magnetoprimed tomato seeds.
TL;DR: Dietary BPA induced oxidative stress, inflammatory response, apoptosis and mitochondrial dysfunction in mouse colons and livers, and the effect of BPA on intestinal and liver function was investigated.
TL;DR: In this paper, the potential of exogenous application of sodium nitroprusside (SNP; 0.1 mM), a nitric oxide (NO) donor, on some growth characteristics, organic metabolites/biomolecules and mineral nutrients of pepper plants subjected to cadmium (Cd) and lead (Pb) stresses (0.1
TL;DR: This study artificially inhibited the activity of cellular catalase enzyme from human liver cells (WRL-68) using 3-Amino-1,2,4-Triazole (3-AT), and CeNPs was used for imparting protective effect against the deleterious effects of elevated cellular H2O2 concentration.
TL;DR: In this article, the mechanism of NO on mitigating the oxidative damage of table grape (Vitis vinifera L.) during storage at 0°C for 60 days was explored by quality, antioxidant enzyme activity and related gene expression.
TL;DR: Investigation of the dual effects of sodium selenite on the physio-biochemical, anatomical and molecular alterations in different Brassicca napus L. cultivars uncovered the protective mechanism of Se by reinforcing the plant morphology, photosynthesis, osmo-protection, redox balance, enzyme activities for ROS-MG detoxification by reducing ROS and MG components.
TL;DR: In this paper, four maize inbred lines (K-tolerant, 90-21-3 and 099; K-sensitive, D937 and 835) were used to analyze leaf photosynthesis, anatomical structure, chloroplast ultrastructure, ROS, and antioxidant activities.
TL;DR: It is postulated that PAS-Na treatment might be a promising method for controlling postharvest browning and prolonging shelf-life of harvested litchi fruit.