Showing papers by "Takashi Saito published in 2014"

Single App knock-in mouse models of Alzheimer's disease

Abstract: Experimental studies of Alzheimer's disease have largely depended on transgenic mice overexpressing amyloid precursor protein (APP). These mice, however, suffer from artificial phenotypes because, in addition to amyloid β peptide (Aβ), they overproduce other APP fragments. We generated knock-in mice that harbor Swedish and Beyreuther/Iberian mutations with and without the Arctic mutation in the APP gene. The mice showed typical Aβ pathology, neuroinflammation and memory impairment in an age-dependent manner.

Black hole lightning due to particle acceleration at subhorizon scales

Abstract: Supermassive black holes with masses of millions to billions of solar masses are commonly found in the centers of galaxies. Astronomers seek to image jet formation using radio interferometry but still suffer from insufficient angular resolution. An alternative method to resolve small structures is to measure the time variability of their emission. Here we report on gamma-ray observations of the radio galaxy IC 310 obtained with the MAGIC (Major Atmospheric Gamma-ray Imaging Cherenkov) telescopes, revealing variability with doubling time scales faster than 4.8 min. Causality constrains the size of the emission region to be smaller than 20% of the gravitational radius of its central black hole. We suggest that the emission is associated with pulsar-like particle acceleration by the electric field across a magnetospheric gap at the base of the radio jet.

Unprecedented study of the broadband emission of Mrk 421 during flaring activity in March 2010

Abstract: A flare from the TeV blazar Mrk 421, occurring in March 2010, was observed for 13 consecutive days from radio to very high energy (VHE, E > 100 GeV) gamma-rays with MAGIC, VERITAS, Whipple, FermiLAT, MAXI, RXTE, Swift, GASP-WEBT, and several optical and radio telescopes. We model the day-scale SEDs with one-zone and two-zone synchrotron self-Compton (SSC) models, investigate the physical parameters, and evaluate whether the observed broadband SED variability can be associated to variations in the relativistic particle population. Flux variability was remarkable in the X-ray and VHE bands while it was minor or not significant in the other bands. The one-zone SSC model can describe reasonably well the SED of each day for the 13 consecutive days. This flaring activity is also very well described by a two-zone SSC model, where one zone is responsible for the quiescent emission while the other smaller zone, which is spatially separated from the first one, contributes to the daily-variable emission occurring in X-rays and VHE gamma-rays. Both the one-zone SSC and the two-zone SSC models can describe the daily SEDs via the variation of only four or five model parameters, under the hypothesis that the variability is associated mostly to the underlying particle population. This shows that the particle acceleration and cooling mechanism producing the radiating particles could be the main one responsible for the broadband SED variations during the flaring episodes in blazars. The two-zone SSC model provides a better agreement to the observed SED at the narrow peaks of the low- and high-energy bumps during the highest activity, although the reported one-zone SSC model could be further improved by the variation of the parameters related to the emitting region itself ($\delta$, $B$ and $R$), in addition to the parameters related to the particle population.

Protein kinase C-η controls CTLA-4–mediated regulatory T cell function

Abstract: Regulatory T (Treg) cells, which maintain immune homeostasis and self-tolerance, form an immunological synapse (IS) with antigen-presenting cells (APCs). However, signaling events at the Treg cell IS remain unknown. Here we show that the kinase PKC-η associated with CTLA-4 and was recruited to the Treg cell IS. PKC-η-deficient Treg cells displayed defective suppressive activity, including suppression of tumor immunity but not of autoimmune colitis. Phosphoproteomic and biochemical analysis revealed an association between CTLA-4-PKC-η and the GIT2-αPIX-PAK complex, an IS-localized focal adhesion complex. Defective activation of this complex in PKC-η-deficient Treg cells was associated with reduced depletion of CD86 from APCs by Treg cells. These results reveal a CTLA-4-PKC-η signaling axis required for contact-dependent suppression and implicate this pathway as a potential cancer immunotherapy target.

Quantitative proteomics analysis of signalosome dynamics in primary T cells identifies the surface receptor CD6 as a Lat adaptor–independent TCR signaling hub

Abstract: T cell antigen receptor (TCR)-mediated activation of T cells requires the interaction of dozens of proteins. Here we used quantitative mass spectrometry and activated primary CD4(+) T cells from mice in which a tag for affinity purification was knocked into several genes to determine the composition and dynamics of multiprotein complexes that formed around the kinase Zap70 and the adaptors Lat and SLP-76. Most of the 112 high-confidence time-resolved protein interactions we observed were previously unknown. The surface receptor CD6 was able to initiate its own signaling pathway by recruiting SLP-76 and the guanine nucleotide-exchange factor Vav1 regardless of the presence of Lat. Our findings provide a more complete model of TCR signaling in which CD6 constitutes a signaling hub that contributes to the diversification of TCR signaling.

MAGIC gamma-ray and multi-frequency observations of flat spectrum radio quasar PKS 1510 089 in early 2012

Abstract: Aims. Amongst more than fifty blazars detected in very high energy (VHE, E> 100 GeV) γ rays, only three belong to the subclass of flat spectrum radio quasars (FSRQs). The detection of FSRQs in the VHE range is challenging, mainly because of their soft spectra in the GeV-TeV regime. MAGIC observed PKS 1510−089 (z = 0.36) starting 2012 February 3 until April 3 during a high activity state in the high energy (HE, E> 100 MeV) γ-ray band observed by AGILE and Fermi. MAGIC observations result in the detection of a source with significance of 6.0 standard deviations (σ). We study the multi-frequency behaviour of the source at the epoch of MAGIC observation, collecting quasi-simultaneous data at radio and optical (GASP-WEBT and F-Gamma collaborations, REM, Steward, Perkins, Liverpool, OVRO, and VLBA telescopes), X-ray (Swift satellite), and HE γ-ray frequencies. Methods. We study the VHE γ-ray emission, together with the multi-frequency light curves, 43 GHz radio maps, and spectral energy distribution (SED) of the source. The quasi-simultaneous multi-frequency SED from the millimetre radio band to VHE γ rays is modelled with a one-zone inverse Compton model. We study two different origins of the seed photons for the inverse Compton scattering, namely the infrared torus and a slow sheath surrounding the jet around the Very Long Baseline Array (VLBA) core. Results. We find that the VHE γ-ray emission detected from PKS 1510−089 in 2012 February-April agrees with the previous VHE observations of the source from 2009 March-April. We find no statistically significant variability during the MAGIC observations on daily, weekly, or monthly time scales, while the other two known VHE FSRQs (3C 279 and PKS 1222+216) have shown daily scale to sub-hour variability. The γ-ray SED combining AGILE, Fermi and MAGIC data joins smoothly and shows no hint of a break. The multi-frequency light curves suggest a common origin for the millimetre radio and HE γ-ray emission, and the HE γ-ray flaring starts when the new component is ejected from the 43 GHz VLBA core and the studied SED models fit the data well. However, the fast HE γ-ray variability requires that within the modelled large emitting region, more compact regions must exist. We suggest that these observed signatures would be most naturally explained by a turbulent plasma flowing at a relativistic speed down the jet and crossing a standing conical shock.

A half-metallic A- and B-site-ordered quadruple perovskite oxide CaCu3Fe2Re2O12 with large magnetization and a high transition temperature.

Abstract: There are only a few transition metal oxides whose conducting electrons show a strong spin polarization at sufficiently high temperatures for spin electronics applications. Here, the authors find that CaCu3Fe2Re2O12 has such spin-polarized conducting electrons and is ferrimagnetic up to 560 K.

Rapid and multiband variability of the TeV bright active nucleus of the galaxy IC 310

Abstract: Context. The radio galaxy IC 310 has recently been identified as a γ-ray emitter based on observations at GeV energies with Fermi-LAT and at very high energies (VHE, E>100 GeV) with the MAGIC telescopes. Originally classified as a head-tail radio galaxy, the nature of this object is subject of controversy since its nucleus shows blazar-like behavior. Aims. In order to understand the nature of IC 310 and the origin of the VHE emission we studied the spectral and flux variability of IC 310 from the X-ray band to the VHEγ-ray regime. Methods. The light curve of IC 310 above 300 GeV has been measured with the MAGIC telescopes from October 2009 to February 2010. Contemporaneous Fermi-LAT data (2008‐2011) in the 10‐500 GeV energy range were also analyzed. In the X-ray regime, archival observations from 2003 to 2007 with XMM-Newton, Chandra, and Swift-XRT in the 0.5‐10 keV band were studied. Results. The VHE light curve reveals several high-amplitude and short-duration flares. Day-to-day flux variability is clearly pr esent (> 5σ). The photon index between 120 GeV and 8 TeV remains at the value ∼ 2.0 during both low and high flux states. The VHE spectral shape d oes not show significant variability, whereas the flux at 1 TeV change s by a factor of∼ 7. Fermi-LAT detected only eightγ-ray events in the energy range 10 GeV‐500 GeV in three years of observation. The measured photon index of = 1.3± 0.5 in the Fermi-LAT range is very hard. The X-ray measurements show strong variability in both flux and photon index. The latter varied from 1.76± 0.07 to 2.55± 0.07. Conclusions. The rapid variability measured inγ-rays and X-rays confirms the blazar-like behavior of IC 310. The multi-TeVγ-ray emission seems to originate from scales of less than 80 Schwarzschild radii (for a black hole mass of 2× 10 8 M⊙) within the compact core of its FR I radio jet with orientation angle 10 ◦ − 38 ◦ . The spectral energy distribution resembles that of an extr eme blazar, albeit the luminosity is more than two

New Mouse Model of Alzheimer’s

Abstract: Amyloid β-peptide (Aβ) accumulation is a key characteristic of Alzheimer’s disease (AD); therefore, mouse models of AD exhibiting Aβ pathology are valuable tools for unraveling disease mechanisms. However, the overexpression of Aβ precursor protein (APP) used in previous mouse models may cause Aβ-independent artifacts that influence data interpretation. To circumvent these problems, we used an APP knock-in (KI) strategy to introduce mutations to the mouse APP gene to develop a new generation of AD mouse models. These new models, termed APPNL-F and APPNL-G-F, have endogenous APP levels and develop robust Aβ amyloidosis, which induce synaptic degeneration and memory impairments. Thus, we suggest that these novel APP KI mice will serve as important tools to elucidate molecular mechanisms of AD.

MAGIC long-term study of the distant TeV blazar PKS 1424+240 in a multiwavelength context

Abstract: Aims. We present a study of the very high energy (VHE; E>100 GeV) -ray emission of the blazar PKS 1424+240 observed with the MAGIC telescopes. The primary aim of this paper is the multiwavelength spectral characterization and modeling of this blazar, which is made particularly interesting by the recent discovery of a lower limit of its re dshift of z≥ 0.6 and makes it a promising candidate to be the most distant VHE source. Methods. The source has been observed with the MAGIC telescopes in VHE rays for a total observation time of∼33.6 h from 2009 to 2011. A detailed analysis of its -ray spectrum and time evolution has been carried out. Moreover, we have collected and analyzed simultaneous and quasi-simultaneous multiwavelength data. Results. The source was marginally detected in VHE rays during 2009 and 2010, and later, the detection was confir med during an optical outburst in 2011. The combined significance of the stacked sa mple is∼7.2�. The differential spectra measured during the different campaigns can be described by steep power laws with the indices ranging from 3.5± 1.2 to 5.0± 1.7. The MAGIC spectra corrected for the absorption due to the extragalactic background light connect smoothly, within systematic errors, with the mean spectrum in 2009-2011 observed at lower energies by the Fermi-LAT. The absorption-corrected MAGIC spectrum is flat with n o apparent turn down up to 400 GeV. The multiwavelength light curve shows increasing flux in radio and optical bands that could point to a common origin from the same region of the jet. The large separation between the two peaks of the constructed non-simultaneous spectral energy distribution also requires an extremely high Doppler factor if an one zone synchrotron self-Compton model is applied. We find that a two-component s ynchrotron self-Compton model describes the spectral energy distribution of the source well, if the source is located at z∼ 0.6.

MAGIC long-term study of the distant TeV blazar PKS 1424+240 in a multiwavelength context

Abstract: We present a study of the very high energy (VHE; E > 100 GeV) gamma-ray emission of the blazar PKS 1424+240 observed with the MAGIC telescopes. The primary aim of this paper is the multiwavelength spectral characterization and modeling of this blazar, which is made particularly interesting by the recent discovery of a lower limit of its redshift of z > 0.6 and makes it a promising candidate to be the most distant VHE source. The source has been observed with the MAGIC telescopes in VHE gamma rays for a total observation time of ~33.6 h from 2009 to 2011. The source was marginally detected in VHE gamma rays during 2009 and 2010, and later, the detection was confirmed during an optical outburst in 2011. The combined significance of the stacked sample is ~7.2 sigma. The differential spectra measured during the different campaigns can be described by steep power laws with the indices ranging from 3.5 +/- 1.2 to 5.0 +/- 1.7. The MAGIC spectra corrected for the absorption due to the extragalactic background light connect smoothly, within systematic errors, with the mean spectrum in 2009-2011 observed at lower energies by the Fermi-LAT. The absorption-corrected MAGIC spectrum is flat with no apparent turn down up to 400 GeV. The multiwavelength light curve shows increasing flux in radio and optical bands that could point to a common origin from the same region of the jet. The large separation between the two peaks of the constructed non-simultaneous spectral energy distribution also requires an extremely high Doppler factor if an one zone synchrotron self-Compton model is applied. We find that a two-component synchrotron self-Compton model describes the spectral energy distribution of the source well, if the source is located at z~0.6.

An ITAM-Syk-CARD9 signalling axis triggers contact hypersensitivity by stimulating IL-1 production in dendritic cells

Abstract: A variety of reactive organic compounds, called haptens, can cause allergic contact dermatitis. However, the innate immune mechanisms by which haptens stimulate dendritic cells (DCs) to sensitize T cells remain unclear. Here we show that the coupling of ITAM-Syk-CARD9 signalling to interleukin-1 (IL-1) secretion in DCs is crucial for allergic sensitization to haptens. Both MyD88 and Caspase recruitment domain-containing protein 9 (CARD9) signalling are required for contact hypersensitivity (CHS). Naive T cells require signals received through IL-1R1-MyD88 for effector differentiation, whereas DCs require CARD9 and spleen tyrosine kinase (Syk) signalling for hapten-induced IL-1α/β secretion and their ability to prime T cells. DC-specific deletion of CARD9, DAP12, Syk or NLRP3, but not MyD88, is sufficient to abolish CHS. All tested haptens, but not irritants, can induce Syk activation, leading to both the CARD9/BCL10-dependent pro-IL-1 synthesis (signal1) and reactive oxygen species-mediated NLRP3 inflammasome activation (signal2), required for IL-1 secretion. These data unveil an innate immune mechanism crucial for allergic contact sensitization to chemical compounds.

Contemporaneous observations of the radio galaxy NGC 1275 from radio to very high energy γ-rays

Abstract: Aims. The radio galaxy NGC1275, recently identified as a very high energy (VHE, >100GeV) -ray emitter by MAGIC, is one of the few non-blazar active galactic nuclei detected in the VHE regime. The purpose of this work is to better understand the origin of the -ray emission and locate it within the galaxy. Methods. We studied contemporaneous multi-frequency observations of NGC1275 and modeled the overall spectral energy distribution. We analyzed unpublished MAGIC observations carried out between October 2009 and February 2010, and the previously published observations taken between August 2010 and February 2011. We studied the multi-band variability and correlations by analyzing data of Fermi-LAT in the 100MeV–100GeV energy band, as well as Chandra (X-ray), KVA (optical), and MOJAVE (radio) data taken during the same period. Results. Using customized Monte Carlo simulations corresponding to early MAGIC stereoscopic data, we detect NGC1275 also in the earlier MAGIC campaign. The flux level and energy spectra are similar to the results of the second campaign. The monthly light curve above 100GeV shows a hint of variability at the 3.6� level. In the Fermi-LAT band, both flux and spectral shape variabilities are reported. The optical light curve is also variable and shows a clear correlation with the -ray flux above 100MeV. In radio, three compact components are resolved in the innermost part of the jet. One of these components shows a similar trend as the Fermi-LAT and KVA light curves. The -ray spectra measured simultaneously with MAGIC and Fermi-LAT from 100MeV to 650GeV can be well fitted either by a log-parabola or by a power-law with a sub-exponential cutoff for the two observation campaigns. A single-zone synchrotron-self-Compton model, with an electron spectrum following a power-law with an exponential cutoff, can explain the broadband spectral energy distribution and the multi-frequency behavior of the source. However, this model suggests an untypical low bulk-Lorentz factor or a velocity alignment closer to the line of sight than the pc-scale radio jet.

Autoantibodies to IgG/HLA class II complexes are associated with rheumatoid arthritis susceptibility

Abstract: Specific HLA class II alleles are strongly associated with susceptibility to rheumatoid arthritis (RA); however, how HLA class II regulates susceptibility to RA has remained unclear. Recently, we found a unique function of HLA class II molecules: their ability to aberrantly transport cellular misfolded proteins to the cell surface without processing to peptides. Rheumatoid factor (RF) is an autoantibody that binds to denatured IgG or Fc fragments of IgG and is detected in 70-80% of RA patients but also in patients with other diseases. Here, we report that intact IgG heavy chain (IgGH) is transported to the cell surface by HLA class II via association with the peptide-binding groove and that IgGH/HLA class II complexes are specifically recognized by autoantibodies in RF-positive sera from RA patients. In contrast, autoantibodies in RF-positive sera from non-RA individuals did not bind to IgGH/HLA class II complexes. Of note, a strong correlation between autoantibody binding to IgG complexed with certain HLA-DR alleles and the odds ratio for that allele's association with RA was observed (r = 0.81; P = 4.6 × 10(-5)). Our findings suggest that IgGH complexed with certain HLA class II alleles is a target for autoantibodies in RA, which might explain why these HLA class II alleles confer susceptibility to RA.

MAGIC observations and multifrequency properties of the flat spectrum radio quasar 3C 279 in 2011

Abstract: Aims. We study the multifrequency emission and spectral properties of the quasar 3C 279 aimed at identifying the radiation processes taking place in the source. Methods. We observed 3C 279 in very-high-energy (VHE, E> 100 GeV) -rays, with the MAGIC telescopes during 2011, for the first ti me in stereoscopic mode. We combined these measurements with observations at other energy bands: in high-energy (HE, E > 100 MeV) -rays from Fermi-LAT; in X-rays from RXTE; in the optical from the KVA telescope; and in the radio at 43 GHz, 37 GHz, and 15 GHz from the VLBA, Metsahovi, and OVRO radio telescopes - along with optical polarisation measurements from the KVA and Liverpool telescopes. We examined the corresponding light curves and broadband spectral energy distribution and we compared the multifrequency properties of 3C 279 at the epoch of the MAGIC observations with those inferred from historical observations. Results. During the MAGIC observations (2011 February 8 to April 11) 3C 279 was in a low state in optical, X-ray, and -rays. The MAGIC observations did not yield a significant detection. The deri ved upper limits are in agreement with the extrapolation of the HE -ray spectrum, corrected for EBL absorption, from Fermi-LAT. The second part of the MAGIC observations in 2011 was triggered by a high-activity state in the optical and -ray bands. During the optical outburst the optical electri c vector position angle (EVPA) showed a rotation of∼ 180 ◦ . Unlike previous cases, there was no simultaneous rotation of the 43 GHz radio polarisation angle. No VHE -rays were detected by MAGIC, and the derived upper limits suggest the presence of a spectral break or curvature between the Fermi-LAT and MAGIC bands. The combined upper limits are the strongest derived to date for the source at VHE and below the level of the previously detected flux by a factor of ∼ 2. Radiation models that include synchrotron and inverse Compton emissions match the optical to -ray data, assuming an emission component inside the broad line region with size R = 1.1× 10 16 cm and magnetic field B = 1.45 G responsible for the high-energy emission, and another one outside the broad line region and the infrared torus (R = 1.5× 10 17 cm and B = 0.8 G) causing the optical and low-energy emission. We also study the optical polarisation in

Multifrequency studies of the peculiar quasar 4c +21.35 during the 2010 flaring activity

Abstract: The discovery of rapidly variable Very High Energy ( VHE; E > 100 GeV). - ray emission from 4C + 21.35 ( PKS 1222+ 216) by MAGIC on 2010 June 17, triggered by the high activity detected by the Fermi Large Area Telescope ( LAT) in high energy ( HE; E > 100 MeV). - rays, poses intriguing questions on the location of the. - ray emitting region in this flat spectrum radio quasar. We present multifrequency data of 4C + 21.35 collected from centimeter to VHE during 2010 to investigate the properties of this source and discuss a possible emission model. The first hint of detection at VHE was observed by MAGIC on 2010 May 3, soon after a gamma- ray flare detected by Fermi-LAT that peaked on April 29. The same emission mechanism may therefore be responsible for both the HE and VHE emission during the 2010 flaring episodes. Two optical peaks were detected on 2010 April 20 and June 30, close in time but not simultaneous with the two gamma- ray peaks, while no clear connection was observed between the X-ray and gamma- ray emission. An increasing flux density was observed in radio and mm bands from the beginning of 2009, in accordance with the increasing gamma- ray activity observed by Fermi-LAT, and peaking on 2011 January 27 in the mm regime ( 230 GHz). We model the spectral energy distributions ( SEDs) of 4C + 21.35 for the two periods of the VHE detection and a quiescent state, using a one-zone model with the emission coming from a very compact region outside the broad line region. The three SEDs can be fit with a combination of synchrotron self-Compton and external Compton emission of seed photons from a dust torus, changing only the electron distribution parameters between the epochs. The fit of the optical/UV part of the spectrum for 2010 April 29 seems to favor an inner disk radius of < six gravitational radii, as one would expect from a prograde-rotating Kerr black hole.

Detection of bridge emission above 50 GeV from the Crab pulsar with the MAGIC telescopes

Abstract: Context The Crab pulsar is the only astronomical pulsed source detected at very high energy (VHE, E > 100 GeV) gamma rays The emission mechanism of VHE pulsation is not yet fully understood, although several theoretical models have been proposed Aims In order to test new models, we measured the light curve and the spectra of the Crab pulsar with high precision by means of deep observations Methods We analyzed 135 h of selected MAGIC data taken between 2009 and 2013 in stereoscopic mode In order to discuss the spectral shape in connection with lower energies, 55 years of Fermi-LAT data were also analyzed Results The known two pulses per period were detected with a significance of 80 sigma and 126 sigma In addition, significant emission was found between the two pulses with 62 sigma Conclusions We discovered the bridge emission above 50 GeV between the two main pulses This emission can not be explained with the existing theories These data can be used for testing new theoretical models

Nucleic acid sensing by T cells initiates Th2 cell differentiation

Abstract: While T-cell responses are directly modulated by Toll-like receptor (TLR) ligands, the mechanism and physiological function of nucleic acids (NAs)-mediated T cell costimulation remains unclear. Here we show that unlike in innate cells, T-cell costimulation is induced even by non-CpG DNA and by self-DNA, which is released from dead cells and complexes with antimicrobial peptides or histones. Such NA complexes are internalized by T cells and induce costimulatory responses independently of known NA sensors, including TLRs, RIG-I-like receptors (RLRs), inflammasomes and STING-dependent cytosolic DNA sensors. Such NA-mediated costimulation crucially induces Th2 differentiation by suppressing T-bet expression, followed by the induction of GATA-3 and Th2 cytokines. These findings unveil the function of NA sensing by T cells to trigger and amplify allergic inflammation.

Evidence for excluding the possibility of d -wave superconducting-gap symmetry in Ba-doped KFe 2 As 2

Abstract: We have investigated the superconducting(SC)-gap anisotropy for several Ba-doped KFe$_2$As$_2$ samples using laser-based angle-resolved photoemission spectroscopy. We show that the SC-gap anisotropy and node positions drastically change with a small amount of Ba doping. Our results totally exclude a possibility of $d$-wave symmetry and strongly suggest that both spin and orbital fluctuations are important for the paring interaction in the Ba-doped K122.

First broadband characterization and redshift determination of the VHE blazar MAGIC J2001+439

Abstract: Aims. We aim to characterize the broadband emission from 2FGL J2001.1+4352, which has been associated with the unknown-redshift blazar MG4 J200112+4352. Based on its gamma-ray spectral properties, it was identified as a potential very high energy (VHE; E> 100 GeV) gamma-ray emitter. We investigate whether this object is aVHE emitter, characterize its gamma-ray spectrum, and study the broadband emission within the one-zone synchrotron self-Compton (SSC) scenario, which is commonly used to describe the emission in blazars. Moreover, we also intend to determine the redshift of this object, which is a crucial parameter for its scientific interpretation. Methods. The source was observed with MAGIC first in 2009 and later in 2010 within a multi-instrument observation campaign. The MAGIC observations yielded 14.8 h of good quality stereoscopic data. Besides MAGIC, the campaign involved, observations with Fermi-LAT, Swift-XRT/UVOT, the optical telescopes KVA, Goddard Robotic Telescope, Galaxy View observatory, Crimean Astrophysical observatory, St. Petersburg observatory, and the Owens Valley Radio Observatory. The object was monitored at radio, optical and gamma-ray energies during the years 2010 and 2011. We characterize the radio to VHE spectral energy distribution and quantify the multiband variability and correlations over short (few days) and long (many months) timescales. We also organized deep imaging optical observations with the Nordic Optical Telescope in 2013 to determine the source redshift. Results. The source, named MAGIC J2001+439, is detected for the first time at VHE with MAGIC at a statistical significance of 6.3σ (E > 70 GeV) during a 1.3 h long observation on 2010 July 16. The multi-instrument observations show variability in all energy bands with the highest amplitude of variability in the X-ray and VHE bands. Besides the variability on few-day timescales, the long-term monitoring of MAGIC J2001+439 shows that, the gamma-ray, optical, and radio emissions gradually decreased on few-month timescales from 2010 through 2011, indicating that at least some of the radio, optical and gamma-ray emission is produced in a single region by the same population of particles. We also determine for the first time the redshift of this BL Lac object through the measurement of its host galaxy during low blazar activity. Using the observational evidence that the luminosities of BL Lac host galaxies are confined to a relatively narrow range, we obtain z = 0.18 ± 0.04. Additionally, we use the Fermi-LAT and MAGIC gamma-ray spectra to provide an independent redshift estimation, z = 0.17 ± 0.10. Using the former (more accurate) redshift value, we adequately describe the broadband emission with a one-zone SSC model for different activity states and interpret the few-day timescale variability as produced by changes in the high-energy component of the electron energy distribution.

Doping evolution of the quasiparticle excitations in heavily hole-doped Ba 1 − x K x Fe 2 As 2 : A possible superconducting gap with sign-reversal between hole pockets

Abstract: To gain insight into the unconventional superconductivity of Fe pnictides with no electron pockets, we measure the thermal conductivity $\ensuremath{\kappa}$ and penetration depth $\ensuremath{\lambda}$ in the heavily hole-doped regime of Ba${}_{1\ensuremath{-}x}$K${}_{x}$Fe${}_{2}$As${}_{2}$. The residual thermal conductivity ${(\ensuremath{\kappa}/T)}_{T\ensuremath{\rightarrow}0\phantom{\rule{0.28em}{0ex}}\mathrm{K}}$ and $T$ dependence of $\ensuremath{\lambda}$ consistently indicate the fully gapped superconductivity at $x=0.76$ and the (line) nodal superconductivity at higher hole concentrations. The magnitude of $\frac{\ensuremath{\kappa}}{T}\phantom{\rule{0.16em}{0ex}}{T}_{c}{|}_{T\ensuremath{\rightarrow}0\phantom{\rule{0.28em}{0ex}}\mathrm{K}}$ and the slope of $\ensuremath{\lambda}(T)$ at low temperatures, both of which are determined by the properties of the low-energy excitations, exhibit a highly unusual nonmonotonic $x$ dependence. These results indicate a dramatic change of the nodal characteristics in a narrow doping range. We suggest that the observed $x$ dependence is naturally explained by a doping crossover of the gap function between the $s$-wave states with and without sign reversal between $\ensuremath{\Gamma}$-centered hole pockets.

B-Cation Order Control of Magnetism in the 1322 Perovskite CaCu3Fe2Nb2O12

Abstract: Cation ordering in the 1322 perovskite CaCu3Fe2Nb2O12 has been investigated by synchrotron X-ray diffraction structure analysis, high-resolution electron microscopy, and magnetic measurements. All of the samples have full 1:3 ordering of Ca and Cu cations at the perovskite A-sites, but the Fe and Nb cations at the B sites show different degrees of ordering depending on the synthesis conditions. A slow-cooled sample has almost complete rock-salt-type 1:1 cation ordering at the B sites, but a rapidly cooled sample has no long-range B-cation arrangement. The ordered compound shows a large saturated magnetization (5.7 μB/formula unit (fu)) below 170 K due to the ferrimagnetism of the A′-site Cu2+ and B-site Fe3+ spins, whereas the B-site disordered form has a small magnetization (<1 μB/fu at 5 K). The degree of B-site cation order thus controls the magnetic behavior of 1322 CaCu3Fe2Nb2O12 perovskites.

Discovery of TeV gamma-ray emission from the Pulsar Wind Nebula 3C 58 by MAGIC

Abstract: Context. The pulsar wind nebula (PWN) 3C 58 is one of the historical very high-energy (VHE; E >100 GeV) -ray source candidates. It is energized by one of the highest spin-down power pulsars known (5% of Crab pulsar) and it has been compared with the Crab nebula because of their morphological similarities. This object was previously observed by imaging atmospheric Cherenkov telescopes (Whipple, VERITAS and MAGIC), although it was not detected, with an upper limit of 2.3 % Crab unit (C.U.) at VHE. It was detected by the Fermi Large Area Telescope (LAT) with a spectrum extending beyond 100 GeV. Aims. We aim to extend the spectrum of 3C 58 beyond the energies reported by the Fermi Collaboration and probe acceleration of particles in the PWN up to energies of a few tens of TeV. Methods. We analyzed 81 hours of 3C 58 data taken in the period between August 2013 and January 2014 with the MAGIC telescopes. Results. We detected VHE -ray emission from 3C 58 with a significance of 5:7 and an integral flux of 0.65% C.U. above 1 TeV. According to our results, 3C 58 is the least luminous VHE -ray PWN ever detected at VHE and has the lowest flux at VHE to date. The di erential energy spectrum between 400 GeV and 10 TeV is well described by a power-law function d /dE= f0(E/1 TeV) with f0 = (2:0 0:4stat 0:6sys) 10 13 cm 2 s 1 TeV 1 and = 2:4 0:2stat 0:2sys. The skymap is compatible with an unresolved source. Conclusions. We report the first significant detection of PWN 3C 58 at TeV energies. We compare our results with the expectations of time-dependent models in which electrons upscatter photon fields. The best representation favors a distance to the PWN of 2 kpc and far-infrared (FIR) values similar to cosmic microwave background photon fields. If we consider an unexpectedly high FIR density, the data can also be reproduced by models assuming a 3.2 kpc distance. A low magnetic field, far from equipartition, is required to explain the VHE data. Hadronic contribution from the hosting supernova remnant (SNR) requires an unrealistic energy budget given the density of the medium, disfavoring cosmic-ray acceleration in the SNR as origin of the VHE -ray emission.

MAGIC upper limits on the GRB 090102 afterglow

Abstract: Indications of a GeV component in the emission from gamma-ray bursts (GRBs) are known since the Energetic Gamma-Ray Experiment Telescope observations during the 1990s and they have been confirmed by the data of the Fermi satellite. These results have, however, shown that our understanding of GRB physics is still unsatisfactory. The new generation of Cherenkov observatories and in particular the MAGIC telescope, allow for the first time the possibility to extend the measurement of GRBs from several tens up to hundreds of GeV energy range. Both leptonic and hadronic processes have been suggested to explain the possible GeV/TeV counterpart of GRBs. Observations with ground-based telescopes of very high energy (VHE) photons (E > 30 GeV) from these sources are going to play a key role in discriminating among the different proposed emission mechanisms, which are barely distinguishable at lower energies. MAGIC telescope observations of the GRB 090102 (z = 1.547) field and Fermi Large Area Telescope data in the same time interval are analysed to derive upper limits of the GeV/TeV emission. We compare these results to the expected emissions evaluated for different processes in the framework of a relativistic blastwave model for the afterglow. Simultaneous upper limits with Fermi and a Cherenkov telescope have been derived for this GRB observation. The results we obtained are compatible with the expected emission although the difficulties in predicting the HE and VHE emission for the afterglow of this event makes it difficult to draw firmer conclusions. Nonetheless, MAGIC sensitivity in the energy range of overlap with space-based instruments (above about 40 GeV) is about one order of magnitude better with respect to Fermi. This makes evident the constraining power of ground-based observations and shows that the MAGIC telescope has reached the required performance to make possible GRB multiwavelength studies in the VHE range.

Discovery of very high energy gamma-ray emission from the blazar 1ES 1727+502 with the MAGIC Telescopes

Abstract: Motivated by the Costamante & Ghisellini (2002) predictions we investigated if the blazar 1ES 1727+502 (z = 0.055) is emitting very high energy (VHE, E>100 GeV) rays. We observed the BL Lac object 1ES 1727+502 in stereoscopic mode with the two MAGIC telescopes during 14 nights between May 6th and June 10th 2011, for a total effective observing time of 12.6 hours. For the study of the multiwavelength spectral energy distribution (SED) we use simultaneous optical R-band data from the KVA telescope, archival UV/optical and X-ray observations by instruments UVOT and XRT on board of the Swift satellite and high energy (HE, 0.1 GeV - 100 GeV) ‐ray data from the Fermi-LAT instrument. We detect, for the first time, VHE ‐ray emission from 1ES 1727+502 at a statistical significance of 5.5 �. The integral flux above 150 GeV is estimated to be (2.1± 0.4)% of the Crab Nebula flux and the de-absorbed VHE spectrum has a photon index of (2.7± 0.5). No significant short-term variability was found in any of the wavebands presented here. We model the SED using a one-zone synchrotron self-Compton model obtaining parameters typical for this class of sources.

Effects of magnesium deficiency on magnesium uptake activity of rice root, evaluated using 28 Mg as a tracer

Abstract: The mechanisms underlying magnesium (Mg) uptake by plant roots remain to be fully elucidated. In particular, there is little information about the effects of Mg deficiency on Mg uptake activity. A Mg uptake kinetic study is essential for better understanding the Mg uptake system. We performed a Mg uptake tracer experiment in rice plants using 28 Mg. Mg uptake was mediated by high- and low-affinity transport systems. The K m value of the high-affinity transport system was approximately 70 μM under Mg-deficient conditions. The Mg uptake activity was promoted by Mg deficiency, which in turn fell to the basal level after 5- min of Mg resupply. The induced uptake rate was inhibited by ionophore treatment, suggesting that an energy-dependent uptake system is enhanced by Mg deficiency. The Mg uptake changes rapidly with Mg conditions in rice, as revealed by a 28 Mg tracer experiment. This technique is expected to be applicable for Mg uptake analyses, particularly in mutants or other lines.

Expression of astrocyte-related receptors in cortical dysplasia with intractable epilepsy.

Abstract: Epilepsy is one of the major neurologic diseases, and astrocytes play important roles in epileptogenesis. To investigate possible roles of astrocyte-related receptors in patients with intractable epilepsy associated with focal cortical dysplasia (FCD) and other conditions, we examined resected epileptic foci from 31 patients, including 23 with FCD type I, IIa, or IIb, 5 with tuberous sclerosis complex, and 3 with low-grade astrocytoma. Control samples were from 21 autopsied brains of patients without epilepsy or neurologic deficits and 5 patients with pathologic gliosis without epilepsy. Immunohistochemical and immunoblot analyses with antibodies against purinergic receptor subtypes P2RY1, P2RY2, P2RY4, potassium channels Kv4.2 and Kir4.1, and metabotropic receptor subtypes mGluR1 and mGluR5 were performed. Anti-glial fibrillary acidic protein, anti-NeuN, and anti-CD68 immunostaining was used to identify astrocytes, neurons, and microglia, respectively. Most glial fibrillary acidic protein-immunopositive astrocyte cells in the brain samples from patients with epilepsy were P2RY1-, P2RY2-, P2RY4-, Kv4.2-, Kir4.1-, mGluR1-, and mGluR5-positive, whereas samples from controls and pathologic gliosis showed lower expression levels of these astrocyte-related receptors. Our findings suggest that, although these receptors are necessary for astrocyte transmission, formation of the neuron-glia network, and other physiologic functions, overexpression in the brains of patients with intractable epilepsy may be associated with activation of intracellular and glio-neuronal signaling pathways that contribute to epileptogenesis.

MAGIC reveals a complex morphology within the unidentified gamma-ray source HESS J1857+026

Abstract: Aims. HESS J1857+026 is an extended TeV gamma-ray source that was discovered by H. E. S. S. as part of its Galactic plane survey. Given its broadband spectral energy distribution and its spatial coincidence with the young energetic pulsar PSR J1856+0245, the source has been put forward as a pulsar wind nebula (PWN) candidate. MAGIC has performed follow-up observations aimed at mapping the source down to energies approaching 100 GeV in order to better understand its complex morphology. Methods. HESS J1857+026 was observed by MAGIC in 2010, yielding 29 h of good quality stereoscopic data that allowed us to map the source region in two separate ranges of energy. Results. We detected very-high-energy gamma-ray emission from HESS J1857+026 with a significance of 12 sigma above 150 GeV. The differential energy spectrum between 100 GeV and 13 TeV is described well by a power law function dN/dE = N-0(E/1TeV)(-Gamma) with N-0 = (5.37 +/- 0.44(stat) +/- 1.5(sys)) X 10(-12) (TeV-1 cm(-2) s(-1)) and Gamma = 2.16 +/- 0.07(stat) +/- 0.15(sys), which bridges the gap between the GeV emission measured by Fermi-LAT and the multi-TeV emission measured by H.E.S.S.. In addition, we present a detailed analysis of the energy-dependent morphology of this region. We couple these results with archival multiwavelength data and outline evidence in favor of a two-source scenario, whereby one source is associated with a PWN, while the other could be linked with a molecular cloud complex containing an HII region and a possible gas cavity.

First broadband characterization and redshift determination of the VHE blazar MAGIC J2001+439

Abstract: We aim to characterize the broadband emission from 2FGL J2001.1+4352, which has been associated with the unknown-redshift blazar MG4 J200112+4352. Based on its gamma-ray spectral properties, it was identified as a potential very high energy (VHE; E > 100 GeV) gamma-ray emitter. The source was observed with MAGIC first in 2009 and later in 2010 within a multi-instrument observation campaign. The MAGIC observations yielded 14.8 hours of good quality stereoscopic data. The object was monitored at radio, optical and gamma-ray energies during the years 2010 and 2011. The source, named MAGIC J2001+439, is detected for the first time at VHE with MAGIC at a statistical significance of 6.3 {\sigma} (E > 70 GeV) during a 1.3-hour long observation on 2010 July 16. The multi-instrument observations show variability in all energy bands with the highest amplitude of variability in the X-ray and VHE bands. We also organized deep imaging optical observations with the Nordic Optical Telescope in 2013 to determine the source redshift. We determine for the first time the redshift of this BL Lac object through the measurement of its host galaxy during low blazar activity. Using the observational evidence that the luminosities of BL Lac host galaxies are confined to a relatively narrow range, we obtain z = 0.18 +/- 0.04. Additionally, we use the Fermi-LAT and MAGIC gamma-ray spectra to provide an independent redshift estimation, z = 0.17 +/- 0.10. Using the former (more accurate) redshift value, we adequately describe the broadband emission with a one-zone SSC model for different activity states and interpret the few-day timescale variability as produced by changes in the high-energy component of the electron energy distribution.

MAGIC reveals a complex morphology within the unidentified gamma-ray source HESS J1857+026

Abstract: HESS J1857+026 is an extended TeV gamma-ray source that was discovered by H.E.S.S. as part of its Galactic plane survey. Given its broadband spectral energy distribution and its spatial coincidence with the young energetic pulsar PSR J1856+0245, the source has been put forward as a pulsar wind nebula (PWN) candidate. MAGIC has performed follow-up observations aimed at mapping the source down to energies approaching 100 GeV in order to better understand its complex morphology. HESS J1857+026 was observed by MAGIC in 2010, yielding 29 hours of good quality stereoscopic data that allowed us to map the source region in two separate ranges of energy. We detected very-high-energy gamma-ray emission from HESS J1857+026 with a significance of $12 \sigma$ above $150$ GeV. The differential energy spectrum between $100$ GeV and $13$ TeV is well described by a power law function $dN/dE = N_0(E/1\textrm{TeV})^{-\Gamma}$ with $N_0 = (5.37 \pm0.44_{stat} \pm1.5_{sys}) \times 10^{-12} (\textrm{TeV}^{-1} \textrm{cm}^{-2}$ $\textrm{ s}^{-1})$ and $\Gamma = 2.16\pm0.07_{stat} \pm0.15_{sys}$, which bridges the gap between the GeV emission measured by Fermi-LAT and the multi-TeV emission measured by H.E.S.S.. In addition, we present a detailed analysis of the energy-dependent morphology of this region. We couple these results with archival multi-wavelength data and outline evidence in favor of a two-source scenario, whereby one source is associated with a PWN, while the other could be linked with a molecular cloud complex containing an HII region and a possible gas cavity.