Showing papers by "Forest Research Institute published in 2007"

Biochemical parameters of plants as indicators of air pollution.

Abstract: In the present study species like Mangifera indica, Linn., Cassia fistula, Linn., and Eucalyptus hybrid were exposed to different air pollution load for short duration (active biomonitoring). Variation in biochemical parameters like chlorophyll, protein, soluble sugar free amino acid, ascorbic acid, nitrate reductase, superoxide dismutase and peroxidase in the leaves were found to be pollution load dependent. These variations can be used as indicators of air pollution for early diagnosis of stress or as a marker for physiological damage to trees prior to the onset of visible injury symptoms. Just by analyzing these biochemical indicators air quality can also be assessed.

Sustainable utilisation of forest biomass for energy - possibilities and problems: policy, legislation, certification, and recommendations and guidelines in the Nordic, Baltic, and other European countries.

Abstract: The substitution of biomass for fossil fuels in energy consumption is a measure to mitigate global warming, as well as having other advantages. Political action plans for increased use exist at both European and national levels. This paper briefly reviews the contents of recommendations, guidelines, and other synthesis publications on sustainable use of forest biomass for energy. Topics are listed and an overview of advantages, disadvantages, and trade-offs between them is given, from the viewpoint of society in general and the forestry and energy sectors in particular. For the Nordic and Baltic countries, the paper also identifies the extent to which wood for energy is included in forest legislation and forest certification standards under the “Programme for the Endorsement of Forest Certification” (PEFC) and the “Forest Stewardship Council” (FSC) schemes. Energy and forest policies at EU and national levels, and European PEFC forest standards are analysed. With respect to energy policies, the utilisation of wood for energy is generally supported in forest policies, but forest legislation is seldom used as a direct tool to encourage the utilisation of wood for energy. Regulations sometimes restrict use for environmental reasons. Forest certification standards include indicators directly related to the utilisation of wood for energy under several criteria, with most occurrences found under environmental criteria. Roles and problems in relation to policy, legislation, certification standards, recommendations and guidelines, and science are discussed.

Ski tourism affects habitat use and evokes a physiological stress response in capercaillie Tetrao urogallus: a new methodological approach

Abstract: 1. Human outdoor recreational activities are increasing and have a significant impact on wildlife. There are few methods suitable for investigating the response of rare and endangered species to human recreational activities, although the impact can be assessed at various scales by measuring both physiological and behavioural responses to disturbance. 2. Capercaillie Tetrao urogallus are suffering strong population declines throughout central Europe. We examined the effects of ski tourism on capercaillie habitat use and adrenocortical activity, measured non-invasively in droppings. 3. During three winters, 2003–06, we radio-tracked 13 capercaillie. In the southern Black Forest in Germany, we sampled 396 droppings of these and additional individuals before and after the start of the ski season. We tested whether the intensity of human winter recreational activities affected home range location and habitat use, and we identified those factors influencing the concentration of corticosterone metabolites (CM) in droppings. 4. Capercaillie used habitats subject to ski tourism. Although the latter did not affect home range location, capercaillie preferred undisturbed forests within their home ranges and avoided areas with high recreation intensity in the ski season. Faecal CM levels of individuals in areas with low recreation intensity were significantly lower than those in areas with moderate or high recreation intensity during the entire study period. 5. We conclude that ski tourism affects both habitat use and endocrine status in capercaillie, with potential negative consequences on body condition and overall fitness. 6. Synthesis and applications. This study demonstrates the relevance of studying wildlife responses at various temporal and spatial scales, and the value of using multiple methods applied to the same individuals to monitor the impact of human recreational activities on a free-ranging species. In order to protect capercaillie populations, we recommend that managers keep forests inhabited by capercaillie free from tourism infrastructure and retain undisturbed forest patches within skiing areas.

Variation in fine root biomass of three European tree species: Beech (Fagus sylvatica L.), Norway spruce (Picea abies L. Karst.), and Scots pine (Pinus sylvestris L.)

Abstract: Fine roots (<2 mm) are very dynamic and play a key role in forest ecosystem carbon and nutrient cycling and accumulation We reviewed root biomass data of three main European tree species European beech, (Fagus sylvatica L), Norway spruce (Picea abies L Karst) and Scots pine (Pinus sylvestris L), in order to identify the differences between species, and within and between vegetation zones, and to show the relationships between root biomass and the climatic, site and stand factors The collected literature consisted of data from 36 beech, 71 spruce and 43 pine stands The mean fine root biomass of beech was 389 g m−2, and that of spruce and pine 297 g m−2 and 277 g m−2, respectively Data from pine stands supported the hypothesis that root biomass is higher in the temperate than in the boreal zone The results indicated that the root biomass of deciduous trees is higher than that of conifers The correlations between root biomass and site fertility characteristics seemed to be species specific

Nematicidal Activity of Plant Essential Oils and Components From Ajowan ( Trachyspermum ammi ), Allspice ( Pimenta dioica ) and Litsea ( Litsea cubeba ) Essential Oils Against Pine Wood Nematode ( Bursaphelenchus Xylophilus )

Abstract: Commercial plant essential oils from 26 plant species were tested for their nematicidal activities against the pinewood nematode, Bursaphelenchus xylophilus. Good nematicidal activity against B. xylophilus was achieved with essential oils of ajowan (Trachyspermum ammi), allspice (Pimenta dioica) and litsea (Litsea cubeba). Analysis by gas chromatography-mass spectrometry led to identification of 12, 6 and 16 major compounds from ajowan, allspice and litsea oils, respectively. These compounds from three plant essential oils were tested individually for their nematicidal activities against the pinewood nematode. LC50 values of geranial, isoeugenol, methyl isoeugenol, eugenol, methyl eugenol and neral against pine wood nematodes were 0.120, 0.200, 0.210, 0.480, 0.517 and 0.525 mg/ml, respectively. The essential oils described herein merit further study as potential nematicides against the pinewood nematode.

Fuzzy-based method for landslide hazard assessment in active seismic zone of Himalaya

Abstract: Landslides in Himalaya cause widespread damage in terms of property and human lives. It the present study, an attempt is made to derive information on causative parameters and preparation of landslide-susceptible map using fuzzy data integration in one of the seismically active region of Garhwal Himalaya that was recently devastated by a huge landslide. High-resolution remotely sensed data products acquired from Indian Remote Sensing Satellite before and after the landslide event were processed to improve interpretability and derivation of causative parameters. Spatial data sets such as lithology, rock weathering, geomorphology, lineaments, drainage, land use, anthropogenic factor, soil type and depth, slope gradient, and slope aspect were integrated using fuzzy gamma operator. The final map was reclassified in to five classes such as highly to lowly susceptible classes based on cumulative cutoff. The result shows around 72% of known landslide areas including the large Uttarkashi landslide in the high and very high susceptibility classes comprising of only 37% of the total area. The precipitation data from ground- and satellite-based observations were compared; the precipitation threshold and the role of seismic activity were analyzed for initiation of landslide.

Mast cells: multipotent local effector cells in atherothrombosis

Abstract: Our understanding of the relationship between the proatherogenic activities of arterial mast cells (MCs) and the development of atherosclerotic lesions is advancing. Atherosclerosis is a chronic inflammatory disease in which cholesterol and other lipids of circulating low-density lipoprotein (LDL) particles accumulate both extracellularly and intracellularly in the innermost layer of the arterial wall, the intima. One prerequisite for the proatherogenic activity of the LDL particles is their retention and proteolytic modification within the extracellular matrix of the intima. Experimental studies with activated chymase-secreting MCs have provided us fundamental insights into the molecular mechanisms of these processes. High-density lipoprotein (HDL) particles, again, remove cholesterol from the intracellular stores and carry it back to the circulation. MC chymase and tryptase actively degrade HDL and thus generate functionally defective particles that are unable to initiate cholesterol efflux from the arterial wall. In advanced atherosclerotic plaques, the accumulated lipids are separated from the circulation by a collagenous cap. By inducing apoptosis of endothelial cells (ECs), subendothelial MCs may induce detachment of ECs from the cap (plaque erosion). Moreover, MCs may weaken the cap if they disturb local collagen turnover by inducing apoptosis of the collagen-secreting smooth muscle cells or when they promote collagen degradation by activating matrix metalloproteinases. Plaques with a weak cap are vulnerable to rupture. The exposed subendothelial tissue at eroded and ruptured sites of plaques triggers local development of a platelet-rich thrombus. As regulators of the collagen-induced platelet activation and fibrin formation/fibrinolysis, the MCs may retard or accelerate the growth of the plaque-associated thrombus and ultimately participate in the wound-healing response of the injured plaque. We propose that by promoting cholesterol accumulation and plaque vulnerability and by locally regulating hemostasis, MCs in atherosclerotic lesions have the potential to contribute to the clinical outcomes of atherosclerosis, such as myocardial infarction and stroke.

The Values and Objectives of Private Forest Owners and Their Influence on Forestry Behaviour: The Implications for Entrepreneurship

Abstract: There are many factors that determine what forestry activities forest owners carry out in their forest properties and that influence whether forest owners engage in entrepreneurial activity. This paper explores whether the values and objectives of forest owners influence their forestry behaviour and their engagement in entrepreneurial activity. This is done through a review of the literature on private forest owners’ typologies based on owners’ objectives. The review reveals that typologies typically divide forest owners into two main groups. The primary objective of the first group of owners is production (of wood and non-wood goods and services) usually, although not exclusively, so as to generate economic activity. The primary objective of the second group is consumption (of wood and non-wood goods and services). There is a tacit assumption in the studies reviewed that goals and objectives do influence forestry behaviour but few studies have actually assessed whether this is the case. The general finding is that forest owners whose objectives are timber production and who are business-oriented are more likely to manage and harvest their stands. No research focusing on the link between owners’ objective and wider entrepreneurial activity in forests was found.

Aortic valve stenosis: an active atheroinflammatory process.

Abstract: Purpose of review To summarize the current understanding of the pathobiology of aortic valve stenosis and portray the major advances in this field. Recent findings Stenotic aortic valves are characterized by atherosclerosis-like lesions, consisting of activated inflammatory cells, including T lymphocytes, macrophages, and mast cells, and of lipid deposits, calcific nodules, and bone tissue. Active mediators of calcification and cells with osteoblast-like activity are present in diseased valves. Extracellular matrix remodeling, including collagen synthesis and elastin degradation by matrix metalloproteinases and cathepsins, contributes to leaflet stiffening. In experimental animals, hypercholesterolemia induces calcification and bone formation in aortic valves, which can be inhibited by statin treatment. The potential of statins to retard progression of aortic valve stenosis has also been recognized in clinical studies; however, further prospective trials are needed. Angiotensin II-forming enzymes are upregulated in stenotic valves. Angiotensin II may participate in profibrotic progression of aortic valve stenosis and may serve as a possible therapeutic target. Summary Recent findings regarding the interaction of inflammatory cells, lipids, mediators of calcification, and renin-angiotensin system in stenotic valves support the current opinion of aortic valve stenosis being an actively regulated disease, potentially amenable to targeted molecular therapy. Evidence from prospective clinical studies is eagerly awaited.

Evidence for a male-produced pheromone in Tetropium fuscum (F.) and Tetropium cinnamopterum (Kirby) (Coleoptera: Cerambycidae)

Abstract: (E)-6,10-dimethyl-5,9-undecadien-2-ol (geranyl acetol), termed here fuscol, was identified as a male-produced pheromone emitted by Tetropium fuscum (F.) and Tetropium cinnamopterum Kirby. In field experiments, traps baited with synthetic fuscol alone were not significantly attractive, but the combination of fuscol plus host volatiles (a synthetic blend of monoterpenes plus ethanol) attracted significantly more male and female T. fuscum and female T. cinnamopterum than did host volatiles alone. This is the first homoterpenoid alcohol to be described in the Cerambycidae, and the first pheromone reported from the sub-family Spondylidinae.

Metabolic changes during adventitious root primordium development in Tectona grandis Linn. f. (teak) cuttings as affected by age of donor plants and auxin (IBA and NAA) treatment

Abstract: Aging of the donor tree decreased adventitious root formation in shoot cuttings of Tectona grandis Linn. f. (teak). Exogenous application of auxins, i.e., a-naphthalene acetic acid (NAA) and indole-3-butyric acid (IBA) has a significant positive effect on the percentage of rooting. The maximum percent rooting was obtained with 4,000 ppm IBA as compared to other treatment. Significant increase in root number was recorded in shoot cuttings treated with 4,000 ppm NAA. The overall rooting response was better in the treatment with IBA rather than with NAA. Further periodic samples (0, 10, 20, and 30 days) were taken to assess the total soluble sugar, starch, protein, and peroxidase (PER) activity in the rooting zone of shoot cuttings of teak during adventitious root formation. Application NAA and IBA to shoot cuttings resulted in an increase in the level of total soluble sugar, starch, protein, and PER-activity in the rooting zone. The stored carbohydrates were utilized during adventitious root formation. Hence, total soluble sugar and starch contents of cuttings, irrespective of age of donor plants, decreased with the passage of time in cuttings planted for rooting. Significant fluctuations were observed in the protein content of cuttings during the time of root induction. There was an increase in the protein content with the passage of time from the day of planting up to its 20th day, followed by a sharp decline in the protein content of cuttings at the 30th day of planting, irrespective of the age of donor plants or the treatment of cuttings with auxins. Irrespective of donor plant age, PER-activity in the cuttings increased from the day of their planting for rooting up to the 20th day, and then declined at its 30th day of planting. It was interesting to note that PER-activity remained higher at all stages in the cuttings of 2-month-old seedlings which rooted profusely as compared to the cuttings of 15- and 30-year-old donor plants those rooted poorly. This study

Analysing transport costs of Danish forest wood chip resources by means of continuous cost surfaces

Abstract: While international markets for woody biomass emerge, growing consumption of wood chips for energy in Denmark is leading to increasing import. With improved competitiveness and unquestionable environmental benefits, domestic wood chip supply is preferable, but measures must be taken to make the supply chain more cost efficient. One of the major contributors to the delivered costs of wood chips is transportation, which is highly determined by the geographical location of forests and energy plants. This paper presents a method based on continuous cost surface mapping using raster-based geographical information systems (GISs). The national wood chip resources have been mapped and for selected bioenergy plants the costs of transporting the annual fuel demand have been modelled using cost distance functions, supply curves and sensitivity analysis. Hence a geographically determined relation of cumulative wood chip resources and their average costs was established. The results may be used for socio-economically sound resource allocation, optimal plant fuel mix, and planning of future energy plants.

Alternative tactics and individual reproductive success in natural associations of the burying beetle, Nicrophorus vespilloides

Abstract: Alternative reproductive tactics can be maintained through different evolutionary avenues. They can be genetically or stochastically determined, in which case they must yield equal fitness, or their use can be conditional, in which case the fitness payoff of alternatives may differ. We attempted to assess the reproductive success of alternative reproductive tactics employed by wild male and female burying beetles in natural associations on carcasses placed in the field. A beetle’s reproductive tactic was defined by its potential involvement in care of larvae, and parentage was assessed using oligolocus DNA fingerprinting of offspring and potential parents. Both in males and in females, alternative tactics yielded significantly different reproductive benefits: subordinate females (brood parasites) and males (satellite males) had considerably lower reproductive success than dominant or uncontested individuals. Joint breeding was too infrequent for statistical inferences, generating intermediate offspring numbers. About 15% of offspring were sired by males not present on the carcass, suggesting that mating away from reproductive resources can produce reproductive benefits to males. Our results, in concert with the observation that beetles using one tactic can be manipulated into employing the alternative, support the notion that Nicrophorus vespilloides uses alternatives conditionally, opportunistically employing lower-benefit tactics when more profitable tactics are not available, or as additional ‘‘on-the-side’’ tactics to bolster reproductive success. Key words: alternative tactics, brood parasitism, burying beetles, communal breeding, DNA fingerprinting, Nicrophorus, parentage, satellite males. [Behav Ecol]

Function and regulation of the complement system in cardiovascular diseases.

Abstract: The complement system plays a central role in innate immunity and also regulates adaptive immunity. The complement system has been demonstrated to contribute to various diseases, including cardiovascular diseases. Complement is extensively activated in atherosclerotic lesions, in arterial aneurysms, and in the myocardium of ischemic and failing hearts. Accumulating evidence shows that limitation of excessive complement activation under these conditions may hold therapeutic value. On the other hand, defects in the classical complement pathway predispose to vasculitis and atherosclerosis, possibly due to ineffective clearance of apoptotic/necrotic cells and abnormal processing of immune complexes. Here, we describe complement activation and regulation in cardiovascular diseases and discuss the evidence derived mainly from experimental animals suggesting that modulation of complement activation may alter the course of these disorders.

Double-Blind Comparison of Escitalopram and Duloxetine in the Acute Treatment of Major Depressive Disorder

Abstract: Background and objective: Escitalopram is the most selective serotonin reuptake inhibitor antidepressant; in contrast, duloxetine inhibits both serotonin and norepinephrine reuptake. Double-blind comparison studies may help guide treatment decisions by revealing the relative benefits of different therapeutic approaches. This study evaluated the efficacy and safety of escitalopram versus duloxetine in the acute treatment of patients with moderate to severe major depressive disorder. Methods: A 1-week, single-blind, placebo lead-in period followed by an 8-week, randomised, double-blind, multicentre, parallel-group comparison was conducted from 20 April 2005 to 10 March 2006 in independent psychiatric research facilities with principal investigators who were board certified in psychiatry. A total of 278 outpatients of 382 patients screened with Diagnostic and Statistical Manual of Mental Disorders (4th edition)-diagnosed major depressive disorder (Montgomery-Asberg Depression Rating Scale [MADRS] total score ≥26) were randomised to the two treatment groups. Eight patients received no medication and were excluded from the safety group. Patients were treated with either escitalopram 10–20 mg/day (fixed at 10 mg/day for the first 4 weeks) or duloxetine 60 mg/day. The primary efficacy variable was change from baseline at week 8 in MADRS total score using the last observation carried forward (LOCF) approach. Efficacy, safety and tolerability measures were prospectively defined in the statistical analysis plan prior to study initiation unless otherwise specifically noted as conducted post hoc. Results: A significantly greater proportion of escitalopram-treated patients completed the 8-week study compared with duloxetine-treated patients (87% vs 69%, respectively; p < 0.01). Mean baseline MADRS total scores were 31.0 for the escitalopram group and 31.6 for the duloxetine group. At week 8, escitalopram treatment resulted in significantly greater improvement compared with duloxetine on the prospectively defined primary efficacy endpoint of mean change from baseline in MADRS total score using the LOCF approach (least-squares mean difference [LSMD] −2.42; 95% CI −4.73, −0.11; p < 0.05). There was no difference between treatment groups in the observed cases (OC) analysis (LSMD −0.32; 95% CI −2.71, 2.07; p = 0.79). Significantly fewer escitalopram-treated patients discontinued because of adverse events compared with duloxetine (2% vs 13%, respectively; p < 0.01). Conclusion: These findings suggest that escitalopram is better tolerated and at least as effective as the serotonin-norepinephrine reuptake inhibitor duloxetine in the treatment of major depressive disorder.

Towards developmental modelling of tree root systems

Abstract: Knowledge of belowground structures and processes is essential for understanding and predicting ecosystem functioning, and consequently in the development of adaptive strategies to safeguard production from trees and woody plants into the future. In the past, research has mainly been concentrated on growth models for the prediction of agronomic or forest production. Newly emerging scientific challenges, e.g. climate change and sustainable development, call for new integrated predictive methods where root systems development will become a key element for understanding global biological systems. The types of input data available from the various branches of woody root research, including biomass allocation, architecture, biomechanics, water and nutrient supply, are discussed with a view to the possibility of incorporating them into a more generic developmental model. We discuss here the main focus of root system modelling to date, including a description of simple allometric biomass models, and biomechanical stress models, and then build in complexity through static growth models towards architecture models. The next progressive and logical step in developing an inclusive developmental model that integrates these modelling approaches is discussed.

Molecular characterisation of the interactions between olmesartan and telmisartan and the human angiotensin II AT1 receptor.

Abstract: Background and purpose: Whereas some angiotensin II (Ang II) type 1 receptor blockers (ARBs) produce surmountable antagonism of AT1 receptors, others such as olmesartan and telmisartan display varying degrees of insurmountability. This study compared the molecular interactions of olmesartan and telmisartan with the human AT1 receptor, using well characterised in vitro methods and model systems. Experimental approach: CHO-K1 cells that stably express human AT1 receptors (CHO-hAT1 cells) were used in several pharmacological studies of olmesartan and telmisartan, including direct radioligand binding and inhibition of Ang II-induced inositol phosphate (IP) accumulation. Key results: Both ARBs were found to be competitive antagonists that displayed high affinity, slow dissociation, and a high degree of insurmountability for the AT1 receptor (the latter greater with olmesartan). Their receptor interactions could be described by a two-step process with the initial formation of a loose complex (IR) and subsequent transformation into a tight binding complex (IR*). In washout experiments, [ 3 H] telmisartan dissociated from the receptor with a half-life of 29 min and the Ang II-mediated IP accumulation response was 50% maximally restored within 24 min, whereas values for [ 3 H] olmesartan were 72 min and 76 min, respectively. Conclusions and implications: The high degree of insurmountability, slow dissociation, and high affinity of olmesartan for its receptor may relate to its ability to stabilise IR* via the carboxyl group of its imidazole core. In comparison, telmisartan displays a less potent interaction with the receptor. British Journal of Pharmacology advance online publication, 18 June 2007; doi:10.1038/sj.bjp.0707323

Biomass and production of fine roots in Japanese forests

Abstract: To better understand the control of fine-root dynamics in Japanese forests, we reviewed studies conducted in Japan on fine-root biomass and production. Most of the data on fine-root biomass were obtained for conifer plantations in limited regions; the average fine-root biomass of dominant trees ranged from ∼50 g m−2 for Pinus species (n = 3) to ∼600 g m−2 for Cryptomeria japonica (n = 4) and Chamaecyparis obtusa (n = 3). These values are comparable with or less than those reported for other temperate forests mainly in North America or Europe. Information on fine-root production in Japanese forests remains limited. Fine-root production accounted for ∼30% of the net primary productivity in two deciduous forests, but similar data was not reported for coniferous forests in Japan. In Japanese forests, slope position is a key parameter controlling fine-root biomass that is greater on upper slopes than on lower slopes, probably because soil resource availability decreases upslope. Studies in manipulated soil environments (e.g., removing throughfall to simulate drought) also suggested that fine-root biomass and production were greatly affected by altered soil environments. Physiological control of fine-root dynamics was recently discussed via anatomical analyses of Chamaecyparis obtusa. Findings from Japanese studies generally support data on fine-root biomass and production obtained from other temperate regions. Further attempts to elucidate the influence of slope position (soil resource availability) on fine-root production would be useful to gain a more detailed understanding of the fine-root dynamics in Japanese forests.

A protocol for generating high numbers of mature and functional human mast cells from peripheral blood

Abstract: Summary Background Mast cells (MCs) are multi-functional effector cells with an essential role in innate immunity and host defence, and under several pathological conditions, such as allergy. Here, we aimed at defining the culture conditions that would allow efficient generation of mature and functional human MCs from their progenitor cells. Methods Human peripheral blood-derived CD34+ progenitor cells were cultured in vitro under serum-free conditions with human stem cell factor for 9 weeks. Growth and differentiation of the cells into MCs were optimized by selected cytokines and a combination of hypoxic and normoxic conditions. MCs were phenotypically characterized by immunocytochemistry, their preformed mediators were quantified, and their functional ability to degranulate and release histamine was tested. Results On average, 20 × 106 mature MCs were generated from 0.5 × 106 progenitor cells during 9 weeks of culture, i.e. at least a 40-fold increase in cell number was achieved. The mature MCs had oval-shaped non-lobular nuclei, contained histamine, heparin, tryptase, chymase, and cathepsin G in their secretory granules, and strongly expressed c-kit (CD117) and Fc epsilon receptor I on their surface. Histamine release from the cells could be brought about by IgE–anti-IgE cross-linkage, compound 48/80, substance P, and anaphylatoxin C3a. The MCs remained functional for several weeks after their maturation. Conclusion This study describes an efficient protocol for generating mature MCs from human peripheral blood with a functional phenotype of connective tissue-type MCs. Use of these cultured human MCs will increase our knowledge and understanding about human MC development and biology in human disease.

Activated Mast Cells Induce Endothelial Cell Apoptosis by a Combined Action of Chymase and Tumor Necrosis Factor-α

Abstract: Objective— Activated mast cells (MCs) induce endothelial cell (EC) apoptosis in vitro and are present at sites of plaque erosions in vivo. To further elucidate the role of MCs in endothelial apoptosis and consequently in plaque erosion, we have studied the molecular mechanisms involved in MC-induced EC apoptosis. Methods and Results— Primary cultures of rat cardiac microvascular ECs (RCMECs) and human coronary artery ECs (HCAECs) were treated either with rat MC releasate (ie, mediators released on MC activation), rat chymase and tumor necrosis factor-α (TNF-α), or with human chymase and TNF-α, respectively. MC releasate induced RCMEC apoptosis by inactivating the focal adhesion kinase (FAK) and Akt-dependent survival signaling pathway, and apoptosis was partially inhibited by chymase and TNF-α inhibitors. Chymase avidly degraded both vitronectin (VN) and fibronectin (FN) produced by the cultured RCMECs. In addition, MC releasate inhibited the activation of NF-κB (p65) and activated caspase-8 and -9. Moreo...

The phylogeographical clade trade: tracing the impact of human-mediated dispersal on the colonization of northern Europe by the oak gallwasp Andricus kollari.

Abstract: Human dispersal of organisms is an important process modifying natural patterns of biodiversity. Such dispersal generates new patterns of genetic diversity that overlie natural phylogeographical signatures, allowing discrimination between alternative dispersal mechanisms. Here we use allele frequency and DNA sequence data to distinguish between alternative scenarios (unassisted range expansion and long range introduction) for the colonization of northern Europe by an oak-feeding gallwasp, Andricus kollari. Native to Mediterranean latitudes from Portugal to Iran, this species became established in northern Europe following human introduction of a host plant, the Turkey oak Quercus cerris. Colonization of northern Europe is possible through three alternative routes: (i) unassisted range expansion from natural populations in the Iberian Peninsula; (ii) unassisted range expansion from natural populations in Italy and Hungary; or (iii) descent from populations imported to the UK as trade goods from the eastern Mediterranean in the 1830s. We show that while populations in France were colonized from sources in Italy and Hungary, populations in the UK and neighbouring parts of coastal northern Europe encompass allozyme and sequence variation absent from the known native range. Further, these populations show demographic signatures expected for large stable populations, rather than signatures of rapid population growth from small numbers of founders. The extent and spatial distribution of genetic diversity in the UK suggests that these A. kollari populations are derived from introductions of large numbers of individuals from each of two genetically divergent centres of diversity in the eastern Mediterranean. The strong spatial patterning in genetic diversity observed between different regions of northern Europe, and between sites in the UK, is compatible with leptokurtic models of population establishment.