Showing papers by "Nagoya University published in 1996"
TL;DR: This biennial Review summarizes much of particle physics, using data from previous editions.
12,798 citations
TL;DR: It is shown that a mutant allele of CCR-5 is present at a high frequency in caucasian populations, but is absent in black populations from Western and Central Africa and Japanese populations, and a 32-base-pair deletion within the coding region results in a frame shift, and generates a non-functional receptor that does not support membrane fusion or infection by macrophage- and dual-tropic HIV-1 strains.
Abstract: HIV-1 and related viruses require co-receptors, in addition to CD4, to infect target cells. The chemokine receptor CCR-5 (ref.1) was recently demonstrated to be a co-receptor for macrophage-tropic (M-tropic) HIV-1 strains, and the orphan receptor LESTR (also called fusin) allows infection by strains adapted for growth in transformed T-cell lines (T-tropic strains). Here we show that a mutant allele of CCR-5 is present at a high frequency in caucasian populations (allele frequency, 0.092), but is absent in black populations from Western and Central Africa and Japanese populations. A 32-base-pair deletion within the coding region results in a frame shift, and generates a non-functional receptor that does not support membrane fusion or infection by macrophage- and dual-tropic HIV-1 strains. In a cohort of HIV-1 infected caucasian subjects, no individual homozygous for the mutation was found, and the frequency of heterozygotes was 35% lower than in the general population. White blood cells from an individual homozygous for the null allele were found to be highly resistant to infection by M-tropic HIV-1 viruses, confirming that CCR-5 is the major co-receptor for primary HIV-1 strains. The lower frequency of heterozygotes in seropositive patients may indicate partial resistance.
2,668 citations
TL;DR: The sequence determination of the entire genome of the Synechocystis sp.
Abstract: The sequence determination of the entire genome of the Synechocystis sp. strain PCC6803 was completed. The total length of the genome finally confirmed was 3,573,470 bp, including the previously reported sequence of 1,003,450 bp from map position 64% to 92% of the genome. The entire sequence was assembled from the sequences of the physical map-based contigs of cosmid clones and of lambda clones and long PCR products which were used for gap-filling. The accuracy of the sequence was guaranteed by analysis of both strands of DNA through the entire genome. The authenticity of the assembled sequence was supported by restriction analysis of long PCR products, which were directly amplified from the genomic DNA using the assembled sequence data. To predict the potential protein-coding regions, analysis of open reading frames (ORFs), analysis by the GeneMark program and similarity search to databases were performed. As a result, a total of 3,168 potential protein genes were assigned on the genome, in which 145 (4.6%) were identical to reported genes and 1,257 (39.6%) and 340 (10.8%) showed similarity to reported and hypothetical genes, respectively. The remaining 1,426 (45.0%) had no apparent similarity to any genes in databases. Among the potential protein genes assigned, 128 were related to the genes participating in photosynthetic reactions. The sum of the sequences coding for potential protein genes occupies 87% of the genome length. By adding rRNA and tRNA genes, therefore, the genome has a very compact arrangement of protein- and RNA-coding regions. A notable feature on the gene organization of the genome was that 99 ORFs, which showed similarity to transposase genes and could be classified into 6 groups, were found spread all over the genome, and at least 26 of them appeared to remain intact. The result implies that rearrangement of the genome occurred frequently during and after establishment of this species.
2,523 citations
TL;DR: It is demonstrated that physiological responses to GDNF require the presence of a novel glycosyl-phosphatidylinositol (GPI)-linked protein (designated GDNFR-α) that is expressed on GDNF-responsive cells and binds GDNF with a high affinity, which supports the hypothesis that GDNF uses a multi-subunit receptor system in which GDN FR-α and Ret function as the ligand-binding and signalling components.
Abstract: Glial-cell-line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Despite the potential clinical and physiological importance of GDNF, its mechanism of action is unknown. Here we show that physiological responses to GDNF require the presence of a novel glycosyl-phosphatidylinositol (GPI)-linked protein (designated GDNFR-alpha) that is expressed on GDNF-responsive cells and binds GDNF with a high affinity. We further demonstrate that GDNF promotes the formation of a physical complex between GDNFR-alpha and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. These findings support the hypothesis that GDNF uses a multi-subunit receptor system in which GDNFR-alpha and Ret function as the ligand-binding and signalling components, respectively.
1,094 citations
TL;DR: The ambient SHH concentration during the late period determines whether ventralized progenitors differentiate into motor neurons or interneurons, thus defining the pattern of neuronal cell types generated in the neural tube.
923 citations
TL;DR: Overproduction of TAB1 enhanced activity of the plasminogen activator inhibitor 1 gene promoter, which is regulated by TGF-β, and increased the kinase activity of TAK1.
Abstract: Transforming growth factor-β (TGF-β) regulates many aspects of cellular function. A member of the mitogen-activated protein kinase kinase kinase (MAPKKK) family, TAK1, was previously identified as a mediator in the signaling pathway of TGF-β superfamily members. The yeast two-hybrid system has now revealed two human proteins, termed TAB1 and TAB2 (for TAK1 binding protein), that interact with TAK1. TAB1 and TAK1 were co-immunoprecipitated from mammalian cells. Overproduction of TAB1 enhanced activity of the plasminogen activator inhibitor 1 gene promoter, which is regulated by TGF-β, and increased the kinase activity of TAK1. TAB1 may function as an activator of the TAK1 MAPKKK in TGF-β signal transduction.
596 citations
TL;DR: In this article, the microstructural and superconducting properties of light rare earth elements (LREs) are reviewed and the flux pinning mechanism is also discussed, on the basis of their study over the last several years, the melt processes for LRE - Ba - Cu - O are described.
Abstract: Unlike Y123 which forms only a stoichiometric compound, the light rare earth elements (LREs: La, Nd, Sm, Eu, Gd) form a solid solution . The presence of such solid solution caused a depression in the superconducting transition temperatures , particularly for La123, Nd123 and Sm123 when they are melt processed in air. Recently, we have found that the of these LRE123 superconductors can greatly be enhanced when they are melt processed in a reduced oxygen atmosphere. Furthermore, values of these superconductors were larger than that of a good quality Y123 superconductor in high magnetic fields at 77 K. In this article, on the basis of our study over the last several years, the melt processes for LRE - Ba - Cu - O are described, the microstructural and superconducting properties of the superconductors are reviewed and the flux pinning mechanism is also discussed.
550 citations
TL;DR: The cloning and characterization of a cDNA encoding a protein kinase that has high sequence homology to members of the mitogen-activated protein kinases (MAPK) kinase kinase (MAPKKK or MEKK) family are described and results suggest that MAP kinase cascades in plants may function in transducing signals in the presence of environmental stress.
Abstract: We describe here the cloning and characterization of a cDNA encoding a protein kinase that has high sequence homology to members of the mitogen-activated protein kinase (MAPK) kinase kinase (MAPKKK or MEKK) family; this cDNA is named cATMEKKI (Arabidopsis thaliana MAP kinase or ERK kinase kinase 1). The catalytic domain of the putative ATMEKK1 protein shows approximately 40% identity with the amino acid sequences of the catalytic domains of MAPKKKs (such as Byr2 from Schizosaccharomyces pombe, Ste11 from Saccharomyces cerevisiae, Bck1 from S. cerevisiae, MEKK from mouse, and NPK1 from tobacco). In yeast cells that overexpress ATMEKK1, the protein kinase replaces Ste11 in responding to mating pheromone. In this study, the expression of three protein kinases was examined by Northern blot analyses: ATMEKK1 (structurally related to MAPKKK), ATMPK3 (structurally related to MAPK), and ATPK19 (structurally related to ribosomal S6 kinase). The mRNA levels of these three protein kinases increased markedly and simultaneously in response to touch, cold, and salinity stress. These results suggest that MAP kinase cascades, which are thought to respond to a variety of extracellular signals, are regulated not only at the posttranslational level but also at the transcriptional level in plants and that MAP kinase cascades in plants may function in transducing signals in the presence of environmental stress.
530 citations
TL;DR: This work proposes a scheme applicable to all species that distinguishes different classes of genes, provides a single name for all genes of a given function and greatly facilitates comparative studies.
513 citations
TL;DR: Results suggest that the APC-DLG complex may participate in regulation of both cell cycle progression and neuronal function.
Abstract: The adenomatous polyposis coli gene (APC) is mutated in familial adenomatous polyposis and in sporadic colorectal tumors, and its product binds to the adherens junction protein β-catenin. Overexpression of APC blocks cell cycle progression. The APC-β-catenin complex was shown to bind to DLG, the human homolog of the Drosophila discs large tumor suppressor protein. This interaction required the carboxyl-terminal region of APC and the DLG homology repeat region of DLG. APC colocalized with DLG at the lateral cytoplasm in rat colon epithelial cells and at the synapse in cultured hippocampal neurons. These results suggest that the APC-DLG complex may participate in regulation of both cell cycle progression and neuronal function.
466 citations
TL;DR: Results indicate the existence of a novel kinase cascade consisting of TAK1, MAPKK6/MKK3, and p38/MPK2, and could be demonstrated to be phosphorylated and activated in vitro by TAKING1, a recently identified MAPKK kinase.
TL;DR: This is the first elucidation of the structure of a conditioned medium factor required for the growth of low-density plant cell cultures.
Abstract: Proliferation of dispersed plant cells in culture is strictly dependent on cell density, and cells in a low-density culture can only grow in the presence of conditioned medium (CM). No known plant hormones have been able to substitute for CM. To quantify the mitogenic activity of CM, we examined conditions for the assay system using mechanically dispersed mesophyll cells of Asparagus officinalis L. and established a highly sensitive bioassay method. By use of this method, the mitogenic activity of CM prepared from asparagus cells was characterized: it was heat-stable, susceptible to pronase digestion, and resistant to glycosidase treatment. On the basis of these results, the mitogenic activity in CM was purified 10(7)-fold by column chromatography, and two factors named phytosulfokine-alpha and -beta (PSK-alpha and PSK-beta) were obtained. By amino acid sequence analysis and mass spectrometry, the structures of these two factors were determined to be sulfated pentapeptide (H-Tyr(SO3H)-Ile-Tyr(SO3H)-Thr-Gln-OH) and sulfated tetrapeptide (H-Tyr(SO3H)-Ile-Tyr(SO3H)-Thr-OH). PSK-alpha and PSK-beta were prepared by chemical synthesis and enzymatic sulfation. The synthetic peptides exhibited the same activity as the natural factors, confirming the structure for PSK-alpha and PSK-beta mentioned above. This is the first elucidation of the structure of a conditioned medium factor required for the growth of low-density plant cell cultures.
TL;DR: The results imply that the blaIMP gene cassettes have been dispersing into various gram-negative rods with the help of the newly identified integron element, which will be helpful for the early recognition of emerging bla IMP-positive clinical isolates which demonstrate consistent resistance to beta-lactams.
Abstract: We applied PCR to the rapid detection of the metallo-beta-lactamase gene, blaIMP, in clinically isolated gram-negative rods. A total of 54 high-level ceftazidime-resistant strains (MICs, > 128 micrograms/ml) were subjected to PCR analyses with the blaIMP-specific primers, since the blaIMP-bearing clinical isolates tested in our previous study always demonstrated high-level resistance to ceftazidime. Twenty-two blaIMP-positive strains including 9 Pseudomonas aeruginosa, 9 Serratia marcescens, 2 Alcaligenes xylosoxidans, 1 Pseudomonas putida, and 1 Klebsiella pneumoniae strains were newly identified from 18 different hospitals in Japan. These strains were mostly isolated from urine samples and showed high-level resistance to almost every cephem, while their levels of resistance to carbapenems were diverse. The PCR analyses with novel integrase gene-specific (intI3) and acc(6')-Ib gene-specific primers suggested that the integron structure found in a large plasmid harbored by S. marcescens AK9373 was also well conserved among blaIMP-positive strains. These results imply that the blaIMP gene cassettes have been dispersing into various gram-negative rods with the help of the newly identified integron element. Thus, the PCR-aided rapid detection will be helpful for the early recognition of emerging blaIMP-positive clinical isolates which demonstrate consistent resistance to beta-lactams.
TL;DR: It is suggested that THC may also exhibit the same physiological and pharmacological properties as the active form of curcumin in vivo by means of the beta-diketone moiety as well as phenolic hydroxy groups.
TL;DR: The method presented is especially effective for selective macrolactonization of omega-hydroxy carboxylic acids.
Abstract: Scandium trifluoromethanesulfonate (triflate), which is commercially available, is a practical and useful Lewis acid catalyst for acylation of alcohols with acid anhydrides or the esterification of alcohols by carboxylic acids in the presence of p-nitrobenzoic anhydrides. The remarkably high catalytic activity of scandium triflate can be used for assisting the acylation by acid anhydrides of not only primary alcohols but also sterically-hindered secondary or tertiary alcohols. The method presented is especially effective for selective macrolactonization of ω-hydroxy carboxylic acids.
TL;DR: The results suggest that OUES may provide an objective, effort-independent estimation of cardiorespiratory functional reserve that is related both to pulmonary dead space and to metabolic acidosis.
01 Jan 1996
TL;DR: In this paper, the authors studied the stability of a fuel jet diffusion flame by using finite-rate chemistry and showed that it is actually an ensemble of instantaneous local premixed, diffusion., and partially premixed flames.
Abstract: Flame stability of a fuel jet diffusion flame was studied numerically by using finite rate chemistry. The flow is time dependent and plane two-dimensional, and the chemical reaction is described by simplified, overall one-step kinetics. The variable parameters are the jet Reynolds number, P Rc o and Damkohler number, Da o : and three types of flame stability behavior were observed depending on values of these parameters. The first one is the local and occasional extinction at the transition point from a laminar to a turbulent flame. When Re 0 is kept at a rather high value and Da 0 is decreased, local extinction at the transition point begins to occur at a certain critical value. The occasional extinction is caused at the instant when the local scalar dissipation rate in the reaction zone becomes too large, producing a rupture in the reaction zone layer. The rupture is quickly connected again to recover the continuous reaction zone layer. As Da 0 is decreased further, however, the frequency of rupture increases, and at another critical value, complete extinction is produced at the transition point, leaving a short, residual rim flame immediately downtream of the injector. This is the second type of flame stability. As Da 0 is decreased further, the third and final stability characteristic is observed: the blow-off of the whole flame from the injector rim. When the flme is extinguished completely at the transition point, most of the injected fuel flows downstream as a fuel jet entraining the surounding air to produce a lifted, turbulent diffusion flame in the downstream flow. This study of the structure of the flame has shown that it is actually an ensemble of instantaneous local premixed, diffusion., and partially premixed flames.
TL;DR: It is shown that the resistivity decreases with increasing applied magnetic field because the spin-dependent tunneling increases as the relative orientation of the magnetization between grains becomes parallel.
Abstract: A mechanism for the large magnetoresistance observed recently in Co-Al-O granular magnetic films is presented. It is shown that the resistivity decreases with increasing applied magnetic field because the spin-dependent tunneling increases as the relative orientation of the magnetization between grains becomes parallel. With this mechanism we are able to account for the dependence of the magnetoresistance on the magnetization and temperature.
TL;DR: It is reported that mutation in the gene for plectin, a cytoskeleton–membrane anchorage protein, is a cause of autosomal recessive muscular dystrophy associated with skin blistering associated withepidermolysis bullosa simplex.
Abstract: We report that mutation in the gene for plectin, a cytoskeleton–membrane anchorage protein, is a cause of autosomal recessive muscular dystrophy associated with skin blistering (epidermolysis bullosa simplex). The evidence comes from absence of plectin by antibody staining in affected individuals from four families, supportive genetic analysis (localization of the human plectin gene to chromosome 8q24.13–qter and evidence for disease segregation with markers in this region) and finally the identification of a homozygous frameshift mutation detected in plectin cDNA. Absence of the large multifunctional cytoskeleton protein plectin can simultaneously account for structural failure in both muscle and skin.
TL;DR: The magnesium-to-calcium ratio of coral skeletons from Ishigaki Island, Ryukyu Islands, Japan, closely tracked sea surface temperature (SST) over an 8-year period shows the potential to provide fast, precise, high-resolution proxies for past tropical SSTs.
Abstract: The magnesium-to-calcium (Mg/Ca) ratio of coral skeletons from Ishigaki Island, Ryukyu Islands, Japan, closely tracked sea surface temperature (SST) over an 8-year period. Measurements were made with the fast technique of inductively coupled plasma-atomic emission spectrometry. The variation of the coral Mg/Ca ratio with SST change is about four times that of the current, widely used coral strontium-to-calcium ratio. The temporal and geographic variation of the seawater Mg2+/Ca2+ ratio probably has little influence on coral Mg/Ca variation. Results indicate that the coral Mg/Ca ratio has the potential to provide fast, precise, high-resolution proxies for past tropical SSTs.
TL;DR: All pigments investigated had strong antioxidative activity in a liposomal system and reduced the formation of malondialdehyde by UVB irradiation and the scavenging effect of hydroxyl radicals and superoxide anion radicals were influenced by their own structures.
TL;DR: This simulation shows a phase transition between the moving phase and the jamming phase at p =1/( m +1) ( p : density of cars, m : the maximum number of sites by which cars advance at each time step).
Abstract: Traffic flow is simulated by one-dimensional cellular automaton (CA) model including cars moving with high speed. The simulation shows a phase transition between the moving phase and the jamming phase at p =1/( m +1) ( p : density of cars, m : the maximum number of sites by which cars advance at each time step). A mean-field theory can reproduce the average velocity found by simulations.
TL;DR: Heating properties of magnetite cationic liposomes (MCL) were investigated in ex vivo experiments using implanted cell pellets using rat glioma T9 cells into which MCL had been incorporated in a petri dish.
Abstract: The effect of hyperthermia on solid glioma tissue formed subcutaneously in the left femoral region of female F344 rats was investigated. Magnetite cationic liposomes (MCLs), which have a positive surface charge, were used as heating mediators for intracellular hyperthermia. MCLs were injected into the solid tumors, which were then subjected to irradiation by an alternating magnetic field (118 kHz, 384 Oe). The rats were divided into four groups, which received no irradiation (control: group I), or irradiation for 30 min given once (group II), twice (group III) or three times (group IV), and the hyperthermic effect on tumor growth was evaluated. Complete tumor regression was observed in 87.5% of the rats in group IV. In the other groups, tumors completely regressed in 20 and 60% of the rats in groups II and III, respectively. Histological observations showed that in group I tumors, MCLs were localized only around the point where they were injected, while in group II tumors they were a little more dispersed. In the cases of group III and IV tumors, however, the distribution of the MCLs was found to be widespread, and necrotic cells were observed throughout almost the entire tumor tissue. The high percentage of complete regression of group IV is considered to be due to this wide distribution of the MCLs. Furthermore, the treated rats showed no severe side-effects. These results suggest that our magnetic particles are potentially effective tools for the treatment of solid tumors.
TL;DR: In this paper, high temperature protonic conductors based on perovskite-type oxides are reviewed concerning their electrical properties and possible applications and defect equilibria for proton formation in these kind of oxides were discussed.
TL;DR: In this paper, the results of measurements of ultra-low-frequency magnetic noise during a large earthquake (Ms=7.1) at Guam of 8 August, 1993 (depth ∼60 km).
Abstract: We report the results of measurements of ultra-low-frequency magnetic noise during a large earthquake (Ms=7.1) at Guam of 8 August, 1993 (depth ∼60 km). The ULF observing system is located in the Guam Island, about 65 km from the epicenter. Several distinct features of this analysis are summarized. (1) We have proposed rather sophisticated statistical analyses (monthly mean, standard deviation) in order to estimate the wave intensity and polarization (i.e. ratio Z/H). (2) A comparison between the ULF wave activity and ΣKp, is useful in distinguishing between the space geomagnetic pulsations and non-space emissions. (3) Then, the use of the ratio (Z/H) is found to be of essential importance in discrimating the emissions presumably of seismic origin from space plasma waves. (4) The statistical analysis of the temporal evolution of this ratio, has yielded that it shows a broad maximum only about one month before the earthquake, and this suggests that the emissions during this period are very likely to be magnetic precursors. (5) The temporal variation of Z component is similar to that for the Loma Prieta earthquake such that it shows a broad maximum ten days ∼ two weeks before the earthquake and another increase a few days before the earthquake. (6) The emissions presumably associated with the earthquake are of noise-like nature, and their main frequency is 0.02 ∼ 0.05 Hz (with maximum intensity ∼0.1 nT).
TL;DR: In this paper, the spring constant of an atomic force microscope (AFM) cantilever is determined by using a large-scale cantilevers fabricated by means of micromachining techniques.
Abstract: Cantilevers fabricated by means of micromachining techniques are usually used for atomic force microscopy. In this paper, the spring constant of an atomic force microscope (AFM) cantilever is determined by using a large-scale cantilever. Since the spring constant of the large-scale cantilever is calibrated accurately, the spring constant of the AFM cantilever is determined precisely by measuring the deflections of both cantilevers simultaneously using heterodyne interferometry. The slope of the force curve gives the spring constant of the AFM cantilever. It is not necessary to measure the dimensions of the AFM cantilever in the proposed method. Although this method is simple, the spring constant of the AFM cantilever is obtained accurately.
TL;DR: Data indicate that RAGE is a central binding site for AGEs formed in vivo and suggest that AGE-beta2M-MP-RAGE interaction likely contributes to the initiation of an inflammatory response in amyloid deposits of long-term hemodialysis patients, a process which may ultimately lead to bone and joint destruction.
Abstract: An important component of amyloid fibrils in dialysis-related amyloidosis is a form of beta2microglobulin modified with advanced glycation end products (AGEs) of the Maillard reaction, known as AGE-beta2M. We demonstrate here that the interaction of AGE-beta2M with mononuclear phagocytes (MPs), cells important in the pathogenesis of the inflammatory arthropathy of dialysis-related amyloidosis, is mediated by the receptor for AGEs, or RAGE. 125I-AGE-beta2M bound to immobilized RAGE or to MPs in a specific, dose-dependent manner (Kd approximately 53.5 and approximately 81.6 nM, respectively), a process inhibited in the presence of RAGE blockade. AGE-beta2M-mediated monocyte chemotaxis was prevented by excess sRAGE or anti-RAGE IgG. Induction of tumor necrosis factor-alpha (TNF) expression by MPs exposed to AGE-beta2M resulted from engagement of RAGE, as appearances of TNF transcripts and TNF antigen release into culture supernatants were prevented by addition of sRAGE, a process mediated, at least in part, by oxidant stress. AGE-beta2M reduced cytochrome c and the elaboration of TNF by MPs was inhibited by N-acetylcysteine. Consistent with these data, immunohistochemical studies of AGE-laden amyloid deposits of a long-term hemodialysis patient revealed positive staining for RAGE in the MPs infiltrating these lesions. These data indicate that RAGE is a central binding site for AGEs formed in vivo and suggest that AGE-beta2M-MP-RAGE interaction likely contributes to the initiation of an inflammatory response in amyloid deposits of long-term hemodialysis patients, a process which may ultimately lead to bone and joint destruction.
TL;DR: Observations suggest that neuropilin is involved in growth, fasciculation, and targeting for a particular groups of axons in mice.
Abstract: Neuropilin (previously A5) is a cell surface glycoprotein that was originally identified in Xenopus tadpole nervous tissues. In Xenopus, neuropilin is expressed on both the presynaptic and postsynaptic elements in the visual and general somatic sensory systems, suggesting a role in neuronal cell recognition. In this study, we identified a mouse homologue of neuropilin and examined its expression in developing mouse nervous tissues. cDNA cloning and sequencing revealed that the primary structure of the mouse neuropilin was highly similar to that of Xenopus and that the extracellular segment of the molecule possessed several motifs that were expected to be involved in cell-cell interaction. Immunohistochemistry and in situ hybridization analyses in mice indicated that the expression of neuropilin was restricted to particular neuron circuits. Neuropilin protein was localized on axons but not on the somata of neurons. The expression of neuropilin persisted through the time when axons were actively growing to form neuronal connections. These observations suggest that neuropilin is involved in growth, fasciculation, and targeting for a particular groups of axons. © 1996 John Wiley & Sons, Inc.