Technische Universität Darmstadt

About: Technische Universität Darmstadt is a education organization based out in Darmstadt, Germany. It is known for research contribution in the topics: Computer science & Context (language use). The organization has 17316 authors who have published 40619 publications receiving 937916 citations. The organization is also known as: Darmstadt University of Technology & University of Darmstadt.

WNT signalling molecules act in axis formation in the diploblastic metazoan Hydra

Abstract: Members of the Wnt/wingless family of secreted proteins act as short-range inducers and long-range organizers during axis formation, organogenesis and tumorigenesis in many developing tissues. Wnt signalling pathways are conserved in nematodes, insects and vertebrates. Despite its developmental significance, the evolutionary origin of Wnt signalling is unclear. Here we describe the molecular characterization of members of the Wnt signalling pathway--Wnt, Dishevelled, GSK3, beta-Catenin and Tcf/Lef--in Hydra, a member of the evolutionarily old metazoan phylum Cnidaria. Wnt and Tcf are expressed in the putative Hydra head organizer, the upper part of the hypostome. Wnt, beta-Catenin and Tcf are transcriptionally upregulated when head organizers are established early in bud formation and head regeneration. Wnt and Tcf expression domains also define head organizers created by de novo pattern formation in aggregates. Our results indicate that Wnt signalling may be involved in axis formation in Hydra and support the idea that it was central in the evolution of axial differentiation in early multicellular animals.

On the astrophysical robustness of the neutron star merger r-process

Abstract: In this study we explore nucleosynthesis in the dynamic ejecta of compact binary mergers. We are particularly interested in the question how sensitive the resulting abundance patterns are to the parameters of the merging system. Therefore, we systematically investigate combinations of neutron star masses in the range from 1.0 to 2.0 M? and, for completeness, we compare the results with those from two simulations of a neutron star black hole merger. The ejecta masses vary by a factor of 5 for the studied systems, but all amounts are (within the uncertainties of the merger rates) compatible with being a major source of the cosmic r-process. The ejecta undergo robust r-process nucleosynthesis which produces all the elements from the second to the third peak in close-to-solar ratios. Most strikingly, this r-process is extremely robust, and all 23 investigated binary systems yield practically identical abundance patterns. This is mainly the result of the ejecta being extremely neutron rich (Ye similar to 0.04) and the r-process path meandering along the neutron drip line so that the abundances are determined entirely by nuclear rather than astrophysical properties. While further questions related to galactic chemical evolution need to be explored in future studies, we consider this robustness together with the ease with which both the second and third peak are reproduced as strong indications that compact binary mergers are prime candidates for the sources of the observed unique heavy r-process component.

Role of the Selective Contacts in the Performance of Lead Halide Perovskite Solar Cells.

Abstract: The effect of electron- and hole-selective contacts in the final cell performance of hybrid lead halide perovskite, CH3NH3PbI3, solar cells has been systematically analyzed by impedance spectroscopy. Complete cells with compact TiO2 and spiro-OMeTAD as electron- and hole-selective contacts have been compared with incomplete cells without one or both selective contacts to highlight the specific role of each contact. It has been described how selective contacts contribute to enhance the cell FF and how the hole-selective contact is mainly responsible for the high Voc in this kind of device. We have determined that the recombination rate is mainly governed by the selective contacts. This fact has important implication for the future optimization of perovskite solar cells. Finally, we have developed a method to analyze the results obtained, and it has been applied for three different electron-selecting materials: TiO2, ZnO, and CdS.

The impact of a negligent G2/M checkpoint on genomic instability and cancer induction.

Abstract: DNA damage responses (DDR) encompass DNA repair and signal transduction pathways that effect cell cycle checkpoint arrest and/or apoptosis. How DDR pathways respond to low levels of DNA damage, including low doses of ionizing radiation, is crucial for assessing environmental cancer risk. It has been assumed that damage-induced cell cycle checkpoints respond to a single double strand break (DSB) but the G2/M checkpoint, which prevents entry into mitosis, has recently been shown to have a defined threshold of 10-20 DSBs. Here, we consider the impact of a negligent G2/M checkpoint on genomic stability and cancer risk.

γH2AX foci analysis for monitoring DNA double-strand break repair: strengths, limitations and optimization

Abstract: DNA double-strand breaks (DSBs) represent an important radiation-induced lesion and impaired DSB repair provides the best available correlation with radiosensitivity. Physical techniques for monitoring DSB repair require high, non-physiological doses and cannot reliably detect subtle defects. One outcome from extensive research into the DNA damage response is the observation that H2AX, a variant form of the histone H2A, undergoes extensive phosphorylation at the DSB, creating gammaH2AX foci that can be visualized by immunofluorescence. There is a close correlation between gammaH2AX foci and DSB numbers and between the rate of foci loss and DSB repair, providing a sensitive assay to monitor DSB repair in individual cells using physiological doses. However, gammaH2AX formation can occur at single-stranded DNA regions which arise during replication or repair and thus does not solely correlate with DSB formation. Here, we present and discuss evidence that following exposure to ionizing radiation, gammaH2AX foci analysis can provide a sensitive monitor of DSB formation and repair and describe techniques to optimize the analysis. We discuss the limitations and benefits of the technique, enabling the procedure to be optimally exploited but not misused.