University of Utah

About: University of Utah is a education organization based out in Salt Lake City, Utah, United States. It is known for research contribution in the topics: Population & Medicine. The organization has 52894 authors who have published 124076 publications receiving 5265834 citations. The organization is also known as: The U & The University of Utah.

Online and Uncivil? Patterns and Determinants of Incivility in Newspaper Website Comments

Abstract: Incivility in public discussions has received increasing attention from academic and popular commentators in recent years. In an effort to better understand the nature and determinants of such incivility, this study examined a 3-week census of articles and comments posted to a local newspaper’s website—totaling more than 300 articles and 6,400 comments. The results of the content analysis show that incivility occurs frequently and is associated with key contextual factors, such as the topic of the article and the sources quoted within the article. We also find that, contrary to popular perceptions, frequent commenters are more civil than are infrequent commenters, and uncivil commenters are no less likely than civil commenters to use evidence in support of their claims.

An integrated-nanophotonics polarization beamsplitter with 2.4 × 2.4 μm2 footprint

Abstract: Researchers used an inverse design algorithm and experimentally demonstrated an integrated polarization beamsplitter with a footprint of 2.4 × 2.4 μm2.

Absence of radius and ulna in mice lacking hoxa-11 and hoxd-11.

Abstract: MICE with targeted disruptions1 in Hoxgenes have been generated to evaluate the role of the Hox complex in determining the mammalian body plan. This complex of 38 genes encodes transcription factors that specify regional information along the embryonic axes. Early in vertebrate evolution an ancestral complex shared with invertebrates was duplicated twice to give rise to the four linkage groups (Hox A, B, C and D)2,3. As a consequence, corresponding genes on the separate linkage groups, called paralogues, are most closely related to each other. Based on sequence similarities, the Hox genes have been subdivided into 13 paralogous groups. The five most 5′ groups (Hox9–13) pattern the posterior region of the vertebrate embryo and the appendicular skeleton4–18. Mice with individual mutations in the paralogous genes hoxa-11 and hoxd-11 have been described15–18. By breeding these two strains together we have generated double mutants which have dramatic phenotypes not apparent in mice homozygous for the individual mutations. The radius and the ulna of the forelimb are almost entirely eliminated, the axial skeleton shows homeotic transformations, and there are severe kidney defects not present in either single mutant. The limb and axial phenotypes are quantitative: as more mutant alleles are added to the genotype, the phenotype becomes progressively more severe. The appendicular skeleton defects suggest that paralogous Hoxgenes function together to specify limb outgrowth and patterning along the proximodistal axis.

ECG T-Wave Patterns in Genetically Distinct Forms of the Hereditary Long QT Syndrome

Abstract: Background The long QT syndrome is an inherited disorder with prolonged ventricular repolarization and a propensity to ventricular tachyarrhythmias and sudden arrhythmic death. Recent linkage studies have demonstrated three separate loci for this disorder on chromosomes 3, 7, and 11, and specific mutated genes for long QT syndrome have been identified on two of these chromosomes. We investigated ECG T-wave patterns (phenotypes) in members of families linked to three genetically distinct forms of the long QT syndrome. Methods and Results Five quantitative ECG repolarization parameters, ie, four Bazett-corrected time intervals (QTonset-c, QTpeak-c, QTc, and Tduration-c, in milliseconds) and the absolute height of the T wave (Tamplitude, in millivolts), were measured in 153 members of six families with long QT syndrome linked to markers on chromosomes 3 (n=47), 7 (n=30), and 11 (n=76). Genotypic data were used to define each family member as being affected or unaffected with long QT syndrome. Affected member...

Characterization of a microfluidic in vitro model of the blood-brain barrier (μBBB)

Abstract: The blood-brain barrier (BBB), a unique selective barrier for the central nervous system (CNS), hinders the passage of most compounds to the CNS, complicating drug development. Innovative in vitro models of the BBB can provide useful insights into its role in CNS disease progression and drug delivery. Static transwell models lack fluidic shear stress, while the conventional dynamic in vitro BBB lacks a thin dual cell layer interface. To address both limitations, we developed a microfluidic blood-brain barrier (μBBB) which closely mimics the in vivo BBB with a dynamic environment and a comparatively thin culture membrane (10 μm). To test validity of the fabricated BBB model, μBBBs were cultured with b.End3 endothelial cells, both with and without co-cultured C8-D1A astrocytes, and their key properties were tested with optical imaging, trans-endothelial electrical resistance (TEER), and permeability assays. The resultant imaging of ZO-1 revealed clearly expressed tight junctions in b.End3 cells, Live/Dead assays indicated high cell viability, and astrocytic morphology of C8-D1A cells were confirmed by ESEM and GFAP immunostains. By day 3 of endothelial culture, TEER levels typically exceeded 250 Ω cm2 in μBBB co-cultures, and 25 Ω cm2 for transwell co-cultures. Instantaneous transient drop in TEER in response to histamine exposure was observed in real-time, followed by recovery, implying stability of the fabricated μBBB model. Resultant permeability coefficients were comparable to previous BBB models, and were significantly increased at higher pH (>10). These results demonstrate that the developed μBBB system is a valid model for some studies of BBB function and drug delivery.