Showing papers by "University of Zagreb published in 2006"

The fourth data release of the Sloan Digital Sky Survey

Abstract: This paper describes the Fourth Data Release of the Sloan Digital Sky Survey (SDSS), including all survey-quality data taken through 2004 June. The data release includes five-band photometric data for 180 million objects selected over 6670 deg2 and 673,280 spectra of galaxies, quasars, and stars selected from 4783 deg2 of those imaging data using the standard SDSS target selection algorithms. These numbers represent a roughly 27% increment over those of the Third Data Release; all the data from previous data releases are included in the present release. The Fourth Data Release also includes an additional 131,840 spectra of objects selected using a variety of alternative algorithms, to address scientific issues ranging from the kinematics of stars in the Milky Way thick disk to populations of faint galaxies and quasars.

An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans.

Abstract: The frequency of micronuclei (MN) in peripheral blood lymphocytes (PBL) is extensively used as a biomarker of chromosomal damage and genome stability in human populations. Much theoretical evidence has been accumulated supporting the causal role of MN induction in cancer development, although prospective cohort studies are needed to validate MN as a cancer risk biomarker. A total of 6718 subjects from of 10 countries, screened in 20 laboratories for MN frequency between 1980 and 2002 in ad hoc studies or routine cytogenetic surveillance, were selected from the database of the HUman MicroNucleus (HUMN) international collaborative project and followed up for cancer incidence or mortality. To standardize for the inter-laboratory variability subjects were classified according to the percentiles of MN distribution within each laboratory as low, medium or high frequency. A significant increase of all cancers incidence was found for subjects in the groups with medium (RR = 1.84; 95% CI: 1.28-2.66) and high MN frequency (RR = 1.53; 1.04-2.25). The same groups also showed a decreased cancer-free survival, i.e. P = 0.001 and P = 0.025, respectively. This association was present in all national cohorts and for all major cancer sites, especially urogenital (RR = 2.80; 1.17-6.73) and gastro-intestinal cancers (RR = 1.74; 1.01-4.71). The results from the present study provide preliminary evidence that MN frequency in PBL is a predictive biomarker of cancer risk within a population of healthy subjects. The current wide-spread use of the MN assay provides a valuable opportunity to apply this assay in the planning and validation of cancer surveillance and prevention programs.

Diagnosis and treatment of atopic dermatitis in children and adults: European Academy of Allergology and Clinical Immunology/American Academy of Allergy, Asthma and Immunology/PRACTALL Consensus Report

Abstract: There are remarkable differences in the diagnostic and therapeutic management of atopic dermatitis practiced by dermatologists and pediatricians in different countries. Therefore, the European Academy of Allergy and Clinical Immunology and the American Academy of Allergy, Asthma and Immunology nominated expert teams who were given the task of finding a consensus to serve as a guideline for clinical practice in Europe as well as in North America. The consensus report is part of the PRACTALL initiative, which is endorsed by both academies.

Analytical calculation of magnetic field distribution in the slotted air gap of a surface permanent-magnet motor using complex relative air-gap permeance

Abstract: We present an analytical method for calculation of no-load magnetic field distribution in the slotted air gap of a surface permanent-magnet (PM) motor with radial or parallel magnetization. The method introduces the notion of complex relative air-gap permeance, calculated from the conformal transformation of the slot geometry, to take into account the effect of slotting. As a result, an accurate solution of both radial and tangential components of the flux density can be obtained which shows excellent agreement with the results of finite-element simulations. As an example of the effectiveness of the model, we present calculations of the back electromotive force and the cogging torque waveforms in a surface PM motor.

The development of cerebral connections during the first 20-45 weeks' gestation.

Abstract: We have correlated data on neuroanatomical organization and magnetic resonance imaging of transient fetal zones shown to contain connectivity elements (growing axons, synapses, dendrites). In the fetal phase, afferent fibres 'wait' within the subplate zone which is the most prominent lamina on histological and magnetic resonance images and is a substrate of endogenous neuronal activity. In early preterm the thalamocortical afferents accumulate within the superficial subplate and grow into cortical plate developing synapses. In late preterm, the resolution of the subplate and growth of cortico-cortical fibres into the cortical plate occur simultaneously with gyration. Both preterm phases characterize the coexistence of endogenous and sensory-driven circuitries and occurrence of the transient electrical phenomena. In neonates, the long cortico-cortical pathways stop growth, and the main histogenetic events are an elaboration of intracortical circuitry and synaptogenesis. In conclusion, the growth of the axonal pathways preterm explains their vulnerability and plasticity. In neonates the vulnerability is related to the intracortical circuitry.

Multiple origins of cultivated grapevine (Vitis vinifera L. ssp. sativa) based on chloroplast DNA polymorphisms.

Abstract: The domestication of the Eurasian grape (Vitis vinifera ssp. sativa) from its wild ancestor (Vitis vinifera ssp. sylvestris) has long been claimed to have occurred in Transcaucasia where its greatest genetic diversity is found and where very early archaeological evidence, including grape pips and artefacts of a 'wine culture', have been excavated. Whether from Transcaucasia or the nearby Taurus or Zagros Mountains, it is hypothesized that this wine culture spread southwards and eventually westwards around the Mediterranean basin, together with the transplantation of cultivated grape cuttings. However, the existence of morphological differentiation between cultivars from eastern and western ends of the modern distribution of the Eurasian grape suggests the existence of different genetic contribution from local sylvestris populations or multilocal selection and domestication of sylvestris genotypes. To tackle this issue, we analysed chlorotype variation and distribution in 1201 samples of sylvestris and sativa genotypes from the whole area of the species' distribution and studied their genetic relationships. The results suggest the existence of at least two important origins for the cultivated germplasm, one in the Near East and another in the western Mediterranean region, the latter of which gave rise to many of the current Western European cultivars. Indeed, over 70% of the Iberian Peninsula cultivars display chlorotypes that are only compatible with their having derived from western sylvestris populations.

Self-medication with antimicrobial drugs in Europe.

Abstract: We surveyed the populations of 19 European countries to compare the prevalence of antimicrobial drug self-medication in the previous 12 months and intended self-medication and storage and to identify the associated demographic characteristics. By using a multistage sampling design, 1,000-3,000 adults in each country were randomly selected. The prevalence of actual self-medication varied from 1 to 210 per 1,000 and intended self-medication from 73 to 449 per 1,000; both rates were high in eastern and southern Europe and low in northern and western Europe. The most common reasons for self-medication were throat symptoms (e.g., dry, inflamed, red, or sore throat, inflamed tonsils, tonsil pain). The main medication sources were pharmacies and medication leftover from previous prescriptions. Younger age, higher education, and presence of a chronic disease were associated with higher rates of self-medication. Attempts to reduce inappropriate self-medication should target prescribers, pharmacists, and the general public.

Chromosomal aberrations and SCEs as biomarkers of cancer risk

Abstract: Previous studies have suggested that the frequency of chromosomal aberrations (CAs), but not of sister chromatid exchanges (SCEs), predicts cancer risk. We have further examined this relationship in European cohorts comprising altogether almost 22,000 subjects, in the framework of a European collaborative project (CancerRiskBiomarkers). The present paper gives an overview of some of the results of the project, especially as regards CAs and SCEs. The results confirm that a high level of CAs is associated with an increased risk of cancer and indicate that this association does not depend on the time between CA analysis and cancer detection, i.e., is obviously not explained by undetected cancer. The present evidence indicates that both chromatid-type and chromosome-type CAs predict cancer, even though some data suggest that chromosome-type CAs may have a more pronounced predictive value than chromatid-type CAs. CA frequency appears to predict cancers at various sites, although there seems to be a particular association with gastrointestinal cancers. SCE frequency does not appear to have cancer predictive value, at least partly due to uncontrollable technical variation. A number of genetic polymorphisms of xenobiotic metabolism, DNA repair, and folate metabolism affect the level of CAs and might collectively contribute to the cancer predictivity of CAs. Other factors that may influence the association between CAs and cancer include, e.g., exposure to genotoxic carcinogens and internal generation of genotoxic species. Although the association between CA level and cancer is seen at the group level, an association probably also exists for the individual, although it is not known if an individual approach could be feasible. However, group level evidence should be enough to support the use of CA analysis as a tool in screening programs and prevention policies in occupational and environmental health.

Identified baryon and meson distributions at large transverse momenta from Au plus Au collisions at root(S)(NN)=200 GeV

Abstract: Transverse momentum spectra of pi(+/-), p, and (p) over bar p up to 12 GeV/c at midrapidity in centrality selected Au + Au collisions at root s(NN) = 200 GeV are presented. In central Au + Au collisions, both pi(+/-) and p((p) over bar) show significant suppression with respect to binary scaling at p(T) greater than or similar to 4 GeV/c. Protons and antiprotons are less suppressed than pi(+/-), in the range 1.5 less than or similar to p(T) less than or similar to 6 GeV/c. The pi(-)/pi(+) and (p) over bar /p ratios show at most a weak pT dependence and no significant centrality dependence. The p/pi ratios in central Au + Au collisions approach the values in p + p and d + Au collisions at p(T) greater than or similar to 5 GeV/c. The results at high p(T) indicate that the partonic sources of pi(+/-), p, and (p) over bar have similar energy loss when traversing the nuclear medium

Recommended nomenclature of epidote-group minerals

Abstract: Epidote-group minerals are monoclinic in symmetry and have topology consistent with space group P21/m and the general formula A2M3(T2O7)(TO4)(O,F)(OH,O). Zoisite is an orthorhombic polymorph of clinozoisite Ca2Al3(Si2O7)(SiO4)O(OH) and is thus not considered a member of the epidote-group. Epidote-group minerals are divided into three subgroups. (1) Members of the clinozoisite subgroup are derived from the mineral clinozoisite Ca2Al3(Si2O7)(SiO4)O(OH) by homovalent substitutions only. The key cation- and anion-sites are A1 = M2+, A2 = M2+, M1 = M3+, M2 = M3+, M3 = M3+, O4 = O2-, O10 = (OH)-. In other words, the dominant valence as listed above must be maintained. (2) Members of the allanite subgroup are REE-rich minerals typified by the eponymous mineral "allanite". This subgroup may be derived from clinozoisite by homovalent substitutions and one coupled heterovalent substitution of the type A2(REE)3+ + M3M2+ → A2Ca2+ + M3M3+. Thus the valences on the key sites are: A1 = M2+, A2 = M3+, M1 = M3+, M2 = M3+, M3 = M2+, O4 = O2-, O10 = (OH)-. (3) Members of the dollaseite subgroup are REE-rich minerals typified by the eponymous mineral "dollaseite". This subgroup may be derived from clinozoisite by homovalent substitutions and two coupled heterovalent substitutions of the type A2(REE)3+ + M3M2+ → A2Ca2+ + M3M3+ and M1M2+ + O4F - → M1M3+ + O4O-2. Thus the valences on the key sites are: A1 = M2+, A2 = M3+, M1 = M2+, M2 = M3+, M3 = M2+, O4 = F-, O10 = (OH)-. The key cation-sites M3 and A1 (and, in principle, M2) determine the root name. In both clinozoisite and allanite subgroups no prefix is added to the root name if M1 = Al. The prefixes ferri, mangani, chromo, and vanado indicate dominant Fe3+, Mn3+, Cr3+, and V3+ on M1, respectively. In the dollaseite subgroup no prefix is added to the root name if M1 = Mg. Otherwise a proper prefix must be attached; the prefixes ferro and mangano indicate dominant Fe2+ and Mn2+ at M1, respectively. The dominant cation on A2 (other than Ca) is treated according to the Extended Levinson suffix designation. This simple nomenclature requires renaming of the following approved species: Niigataite (old) = clinozoisite-(Sr) (new), hancockite (old) = epidote-(Pb) (new), tweddillite (old) = manganipiemontite-(Sr) (new). Minor modifications are necessary for the following species: Strontiopiemontite (old) = piemon- tite-(Sr) (new), androsite-(La) (old) = manganiandrosite-(La) (new). Before a mineral name can be assigned, the proper subgroup has to be determined. The determination of a proper subgroup is made by the dominating valence at M3, M1, and A2 expressed as M2+ and or M3+, not by a single, dominant ion (i.e., Fe2+, or Mg, or Al). In addition, the dominant valence on O4: X - or X 2- must be ascertained. (M2+)A2 > 0.50, (M3+)M3 > 0.50 → clinozoisite subgroup, (M3++ M4+)A2 > 0.50, (M2+)M3 > 0.50 → allanite subgroup, {(M2+)M3+M1 - (M3++ M4+)A2 } > 0.50 and (X - )O4 > 0.5 → dollaseite subgroup. Coupled heterovalent substitutions in epidote-group minerals require a special application of the so-called 50 % rule in solid-solution series. (1) Clinozoisite subgroup: The dominant trivalent cation on M3 determines the name, whereas the A2 cation appearing in the suffix has to be selected from among the diva- lent cations. (2) Allanite and dollaseite subgroups: For the sites involved in the charge compensation of a heterovalent substitution in A2 and O4 (i.e. M3 in the allanite subgroup; M3 and M1 in the dollaseite subgroup), identification of the relevant end-member formula must take into account the dominant divalent charge-compensating octahedral cation (M2+) and not the dominant cation

Measuring forecast skill: is it real skill or is it the varying climatology?

Abstract: It is common practice to summarize the skill of weather forecasts from an accumulation of samples spanning many locations and dates. In calculating many of these scores, there is an implicit assumption that the climatological frequency of event occurrence is approximately invariant over all samples. If the event frequency actually varies among the samples, the metrics may report a skill that is different from that expected. Many common deterministic verification metrics, such as threat scores, are prone to mis-reporting skill, and probabilistic forecast metrics such as the Brier skill score and relative operating characteristic skill score can also be affected. Three examples are provided that demonstrate unexpected skill, two from synthetic data and one with actual forecast data. In the first example, positive skill was reported in a situation where metrics were calculated from a composite of forecasts that were comprised of random draws from the climatology of two distinct locations. As the difference in climatological event frequency between the two locations was increased, the reported skill also increased. A second example demonstrates that when the climatological event frequency varies among samples, the metrics may excessively weight samples with the greatest observational uncertainty. A final example demonstrates unexpectedly large skill in the equitable threat score of deterministic precipitation forecasts. Guidelines are suggested for how to adjust skill computations to minimize these effects. Copyright © 2006 Royal Meteorological Society

Alzheimer-like changes in protein kinase B and glycogen synthase kinase-3 in rat frontal cortex and hippocampus after damage to the insulin signalling pathway.

Abstract: The insulin-resistant brain state is related to late-onset sporadic Alzheimer's disease, and alterations in the insulin receptor (IR) and its downstream phosphatidylinositol-3 kinase signalling pathway have been found in human brain. These findings have not been confirmed in an experimental model related to sporadic Alzheimer's disease, for example rats showing a neuronal IR deficit subsequent to intracerebroventricular (i.c.v.) treatment with streptozotocin (STZ). In this study, western blot analysis performed 1 month after i.c.v. injection of STZ showed an increase of 63% in the level of phosphorylated glycogen synthase kinase-3alpha/beta (pGSK-3alpha/beta) protein in the rat hippocampus, whereas the levels of the unphosphorylated form (GSK-3alpha/beta) and protein kinase B (Akt/PKB) remained unchanged. Three months after STZ treatment, pGSK-3alpha/beta and Akt/PKB levels tended to decrease (by 8 and 9% respectively). The changes were region specific, as a different pattern was found in frontal cortex. Structural alterations were also found, characterized by beta-amyloid peptide-like aggregates in brain capillaries of rats treated with STZ. Similar neurochemical changes and cognitive deficits were recorded in rats treated with i.c.v. 5-thio-d-glucose, a blocker of glucose transporter (GLUT)2, a transporter that is probably involved in brain glucose sensing. The IR signalling cascade alteration and its consequences in rats treated with STZ are similar to those found in humans with sporadic Alzheimer's disease, and our results suggest a role for GLUT2 in Alzheimer's pathophysiology.

HDDD2 is a familial frontotemporal lobar degeneration with ubiquitin‐positive, tau‐negative inclusions caused by a missense mutation in the signal peptide of progranulin

Abstract: Objective: Familial autosomal dominant frontotemporal dementia with ubiquitin-positive, tau-negative inclusions in the brain linked to 17q21-22 recently has been reported to carry null mutations in the progranulin gene (PGRN). Hereditary dysphasic disinhibition dementia (HDDD) is a frontotemporal dementia with prominent changes in behavior and language deficits. A previous study found significant linkage to chromosome 17 in a HDDD family (HDDD2), but no mutation in the MAPT gene. Longitudinal follow-up has enabled us to identify new cases and to further characterize the dementia in this family. The goals of this study were to develop research criteria to classify the different clinical expressions of dementia observed in this large kindred, to identify the causal mutation in affected individuals and correlate this with phenotypic characteristics in this pedigree, and to assess the neuropathological characteristics using immunohistochemical techniques. Methods: In this study we describe a detailed clinical, pathological and mutation analysis of the HDDD2 kindred. Results: Neuropathologically, HDDD2 represents a familial frontotemporal lobar degeneration with ubiquitin-positive, taunegative inclusions (FTLD-U). We developed research classification criteria and identified three distinct diagnostic thresholds, which helped localize the disease locus. The chromosomal region with the strongest evidence of linkage lies within the minimum critical region for FTLD-U. Sequencing of each exon of the PGRN gene led to the identification of a novel missense mutation, Ala-9 Asp, within the signal peptide. Interpretation: HDDD2 is an FTLD-U caused by a missense mutation in the PGRN gene that cosegregates with the disease and with the disease haplotype in at-risk individuals. This mutation is the first reported pathogenic missense mutation in the signal peptide of the PGRN gene causing FTLD-U. In light of the previous reports of null mutations and its position in the gene, two possible pathological mechanisms are proposed: (1) the protein may accumulate within the endoplasmic reticulum due to inefficient secretion; and (2) mutant RNA may have a lower expression because of degradation via nonsense-mediated decay. Ann Neurol 2006;60:314 –322

Main Determinants of Foreign Direct Investment in the Southeast European Countries

Abstract: The growth of foreign direct investments (FDI) in the world has been significant in recent years. Between 1990 and 2000 worldwide FDI inflows increased more than five times, and since 2000 they have declined. During the period of FDI expansion, growth was especially strong from 1997 onward. However, most of the FDI transactions were between the developed countries. The distribution of FDI is unequal and less-developing countries face difficulties in attracting FDI. Despite the fact that FDI is increasingly important to developing countries, over the past few years the share of the developing countries in worldwide FDI inflows has been declining. The paper analyses geographical and sector distribution of FDI in the Southeast European countries (SEEC) and compares its amount with that in Central East European countries. According to economic theory, FDI towards developing countries flows for labor-intensive and low-technology production, while towards developed states, it flows for high-technology production. Identification of determining factors of FDI is a complex problem which depends on several characteristics specific for each country, sectors, and companies. All those factors could be grouped in three broad categories: economic policy of host country, economic performance, and attractiveness of national economy. On the desegregated level, FDI depends on size and growth potential of a national economy, natural resources endowments and quality of workforce, openness to international trade and access to international markets, and quality of physical, financial, and technological infrastructure. An important question is how SEEC can attract more foreign investment. To find the answer, this paper uses data on FDI inflows to SEEC to determine the main host country determinants of FDI and provides regression-based estimation of determinants of FDI. Using a sample of SEEC and panel data techniques, the determinants of FDI in this part of Europe are investigated. The paper researches the relationship between FDI, GDP, GDP per capita, number of inhabitants, trade openness, inflation, external debt, and information and communication technology sectors. For SEEC, FDI inflows are largely dependent on the completion of the privatization process and in this paper we include the level of private sector and privatization as explanatory variables. Our findings suggest that certain variables such as privatization and trade regime, as well as the density of infrastructure, appear to be robust under different specifications. A positive significance of the agglomeration factor is also observed, confirming the relevant theoretical propositions. However, certain differential variables, such as the privatization, could not be fully captured due to the statistical homogeneity of the sample.

State-of-the-art in the monolithic catalysts/reactors

Abstract: According to some authors development of monolithic catalysts and/or reactors has been one of major achievements in the field of heterogeneous catalysis and chemical reaction engineering of the recent years. This work is aimed at pointing out the advantages of monolithic catalysts and/or reactors with respect to the conventional ones, with particular focus on the integral approach to the catalyst and reactor design. The paper is divided into several parts. The first part gives basic definitions and classification of monolithic catalysts, including basic features of the monoliths and factors that have proceeded to the development and application of the monolith structures. It is explained that monoliths belong to the class of the catalytic reaction systems, where usual differences between catalysts and reactors, arising from their action level, are diminishing. Second part of paper is devoted to the preparation of monolithic catalysts. Next part deals with their commercial application with particular emphasis on the less known applications, and those which are still under development. The paper concludes with forecast of potential monoliths applications and ends up with the future research priorities and directions.

Chromosomal Aberrations and Cancer Risk: Results of a Cohort Study from Central Europe

Abstract: A high level of chromosomal aberrations in peripheral blood lymphocytes may be an early marker of cancer risk, but data on risk of specific cancers and types of chromosomal aberrations (chromosome type and chromatid type) are limited. A total of 6,430 healthy individuals from nine laboratories in Croatia, Hungary, Lithuania, Poland, and Slovakia, included in chromosomal aberration surveys performed during 1978-2002, were followed up for cancer incidence or mortality for an average of 8.5 years; 200 cancer cases were observed. Compared with that for the low-tertile level of chromosomal aberrations, the relative risks of cancer for the medium and high tertiles were 1.78 (95% confidence interval: 1.19, 2.67) and 1.81 (95% confidence interval: 1.20, 2.73), respectively. The relative risk for chromosome-type aberrations above versus below the median was 1.50 (95% confidence interval: 1.12, 2.01), while that for chromatid-type aberrations was 0.97 (95% confidence interval: 0.72, 1.31). The analyses of risk of specific cancers were limited by small numbers, but the association was stronger for stomach cancer. This study confirms the previously reported association between level of chromosomal aberrations and cancer risk and provides novel information on the type of aberrations more strongly predictive of cancer risk and on the types of cancer more strongly predicted by chromosomal aberrations.

Direct observation of dijets in central Au+Au collisions at sNN=200GeV

Abstract: The STAR Collaboration at the Relativistic Heavy Ion Collider reports measurements of azimuthal correlations of high transverse momentum (p(T)) charged hadrons in Au+Au collisions at higher p(T) than reported previously. As p(T) is increased, a narrow, back-to-back peak emerges above the decreasing background, providing a clear dijet signal for all collision centralities studied. Using these correlations, we perform a systematic study of dijet production and suppression in nuclear collisions, providing new constraints on the mechanisms underlying partonic energy loss in dense matter.

Minijet deformation and charge-independent angular correlations on momentum subspace (eta, phi) in Au-Au collisions at root S-NN=130 GeV

Abstract: Measurements of two-particle correlations on angular difference variables η1 − η2 (pseudorapidity) and φ1 − φ2 (azimuth) are presented for all primary charged hadrons with transverse momentum 0.15 ≤ p_t ≤ 2 GeV/c and |η| ≤ 1.3 from Au-Au collisions at √s_(NN) = 130 GeV. Large-amplitude correlations are observed over a broad range in relative angles where distinct structures appear on the same-side and away-side (i.e., relative azimuth less than π/2 or greater than π/2). The principal correlation structures include that associated with elliptic flow plus a strong, same-side peak. It is hypothesized that the latter results from correlated hadrons associated with semi-hard parton scattering in the early stage of the heavy-ion collision which produces a jet-like correlation peak at small relative angles. The width of the jet-like peak on η1 − η2 increases by a factor 2.3 from peripheral to central collisions, suggesting strong coupling of semi-hard scattered partons to a longitudinally-expanding medium. The new methods of jet analysis introduced here provide access to scattered partons at low transverse momentum well below the kinematic range where perturbative quantum chromodynamics and standard fragmentation models are applicable.

Comparison of Composite Curing Parameters: Effects of Light Source and Curing Mode on Conversion, Temperature Rise and Polymerization Shrinkage

Abstract: Clinical Relevance The use of a low intensity light source for photopolymerization based on LED technology provides equivalent final degree conversion with possible flow of the resin composite, similar to when QTH technology is used. At the same time, the lower temperature rise in the sample and the more favorable development of shrinkage kinetics compared to the higher intensities of halogen light may aid in maintaining marginal adaptation while avoiding possible thermal injury.

Forward neutral pion production in p+p and d+Au collisions at root(S)(NN)=200 GeV

Abstract: Measurements of the production of forward pi(0) mesons from p+p and d+Au collisions at root s(NN) = 200 GeV are reported. The p+p yield generally agrees with next-to-leading order perturbative QCD calculations. The d+Au yield per binary collision is suppressed as eta increases, decreasing to similar to 30% of the p+p yield at =4.00, well below shadowing expectations. Exploratory measurements of azimuthal correlations of the forward pi(0) with charged hadrons at eta approximate to 0 show a recoil peak in p+p that is suppressed in d+Au at low pion energy. These observations are qualitatively consistent with a saturation picture of the low-x gluon structure of heavy nuclei.

Children's exposure to environmental pollutants and biomarkers of genetic damage. II. Results of a comprehensive literature search and meta-analysis

Abstract: The present review is based on findings from 178 publications retrieved through an extensive search of the MedLine/PubMed database for a 25 years time period (1980-2004) and 10 manually identified papers. Among the cytogenetic biomarkers that are frequently used in field studies, chromosome aberrations (CA) and micronuclei (MN) but not sister chromatid exchanges (SCE) were found consistently increased in children exposed to environmental pollutants. Meta-analysis of the studies reporting SCE in cord blood showed similar levels of SCE in exposed and in non-exposed newborns. Exposure to airborne pollutants, soil and drinking water contaminants, mostly increased CA and, to a lesser extent, MN levels in children. The effect of exposure to airborne urban pollutants was consistently reported by field studies measuring DNA, albumin and hemoglobin adducts. Prenatal (in utero) and postnatal exposure (environmental tobacco smoke, ETS) to tobacco smoke compounds were associated with increased frequencies of DNA and hemoglobin adducts and CA. The limited number of field studies measuring DNA fragmentation (Comet assay), hypoxanthine-guanine phosphoribosyltransferase (HPRT) and the glycophorinA (GPA) mutation frequency in environmentally exposed children precluded a meaningful evaluation of the usefulness of these assays. Meta-analyses performed in children exposed to ETS and in newborns exposed in utero to their mothers' smoke showed 1.3 and 7 times higher levels of hemoglobin adducts compared to referent subjects, respectively. These increases are consistent with the epidemiological evidence of higher lung cancer risks reported in adults who had never smoked and were exposed to ETS during childhood and with 7-15 times higher lung cancer risks reported in smokers than in non-smokers. Higher levels of PAH-DNA adducts were found in fetal than in maternal tissue, suggesting a specific susceptibility of the fetus to this class of ubiquitous environmental pollutants. According to these findings, future research and biomonitoring programs on children would greatly benefit from the inclusion of selected biomarkers that could provide biologically based evidence for the identification of intervention priorities in environmental health.

Methylglyoxal in food and living organisms.

Abstract: Methylglyoxal (MG) is a highly reactive alpha-oxoaldehyde formed endogenously in numerous enzymatic and nonenzymatic reactions. It modifies arginine and lysine residues in proteins forming advanced glycation end-products such as N(delta)-(5-methyl-4-imidazolon-2-yl)-L-ornithine (MG-H1), 2-amino-5-(2-amino-5-hydro-5-methyl-4-imidazolon-1-yl)pentanoic acid (MG-H2), 2-amino-5-(2-amino-4-hydro-4-methyl-5-imidazolon-1-yl)pentanoic acid (MG-H3), argpyrimidine, N(delta)-(4-carboxy-4,6-dimethyl-5,6-dihydroxy-1,4,5,6-tetrahydropyrimidine-2-yl)-L-ornithine (THP), N(epsilon)-(1-carboxyethyl)lysine (CEL), MG-derived lysine dimer (MOLD), and 2-ammonio-6-({2-[4-ammonio-5-oxido-5-oxopently)amino]-4-methyl-4,5-dihydro-1H-imidazol-5-ylidene}amino)hexanoate (MODIC), which have been identified in vivo and are associated with complications of diabetes and some neurodegenerative diseases. In foodstuffs and beverages, MG is formed during processing, cooking, and prolonged storage. Fasting and metabolic disorders and/or defects in MG detoxification processes cause accumulation of this reactive dicarbonyl in vivo. In addition, the intake of low doses of MG over a prolonged period of time can cause degenerative changes in different tissues, and can also exert anticancer activity. MG in biological samples can be quantified by HPLC or GC methods with preliminary derivatization into more stable chromophores and/or fluorophores, or derivatives suitable for determination by MS by use of diamino derivatives of benzene and naphthalene, 6-hydroxy-2,4,5-triaminopyrimidine, cysteamine, and o-(2,3,4,5,6-pentafluorobenzyl) hydroxylamine. The methods include three basic steps: deproteinization, incubation with derivatization agent, and chromatographic analysis with or without preliminary extraction of the formed products.

Safety and efficacy of botulinum toxin type A following long‐term use

Abstract: Botulinum toxin serotype A (BoNT-A) has long heritage of use leading to confidence in its safety and efficacy. The application of BoNT-A does not lead to persistent histological changes in the nerve terminal or the target muscle. Clinical trials defined the safety and tolerability profile of BoNT-A across common therapeutic indications and showed an incidence of adverse events of approximately 25% in the BoNT-A-treated group compared with 15% in the control group. Focal weakness was the only adverse event to occur more often following BoNT-A treatment. Long-term BoNT-A administration has been assessed in various treatment settings, with the level and duration of BoNT-A efficacy response being maintained over repeated rounds of injection with no major safety concerns. The treatment of children with cerebral palsy often require long-term, repeated, multimuscle BoNT-A injections that lead to the administration of comparably higher toxin doses. Despite the high total body doses used, their distribution over multiple muscles and injection sites means that systemic side effects are rare. Recent formulation changes have reduced the incidence of antibody development following treatment with BOTOX. These findings show long-term BoNT-A treatment to be both safe and efficacious for a wide variety of indications.