A roadmap for the Human Developmental Cell Atlas
Muzlifah Haniffa,Muzlifah Haniffa,Deanne Taylor,Deanne Taylor,Sten Linnarsson,Bruce J. Aronow,Gary D. Bader,Roger A. Barker,Pablo G. Camara,J. Gray Camp,Alain Chédotal,Andrew J. Copp,Heather C. Etchevers,Paolo Giacobini,Berthold Göttgens,Guoji Guo,Ania Hupalowska,Kylie R. James,Emily Kirby,Arnold R. Kriegstein,Joakim Lundeberg,John C. Marioni,Kerstin B. Meyer,Kathy K. Niakan,Kathy K. Niakan,Mats Nilsson,Bayanne Olabi,Dana Pe'er,Aviv Regev,Aviv Regev,Aviv Regev,Jennifer Rood,Orit Rozenblatt-Rosen,Orit Rozenblatt-Rosen,Rahul Satija,Sarah A. Teichmann,Sarah A. Teichmann,Barbara Treutlein,Roser Vento-Tormo,Simone Webb +39 more
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TLDR
The Human Developmental Cell Atlas (HDCA) project as discussed by the authors aims to create a comprehensive reference map of cells during development by mapping and modelling human development using state-of-the-art technologies.Abstract:
The Human Developmental Cell Atlas (HDCA) initiative, which is part of the Human Cell Atlas, aims to create a comprehensive reference map of cells during development. This will be critical to understanding normal organogenesis, the effect of mutations, environmental factors and infectious agents on human development, congenital and childhood disorders, and the cellular basis of ageing, cancer and regenerative medicine. Here we outline the HDCA initiative and the challenges of mapping and modelling human development using state-of-the-art technologies to create a reference atlas across gestation. Similar to the Human Genome Project, the HDCA will integrate the output from a growing community of scientists who are mapping human development into a unified atlas. We describe the early milestones that have been achieved and the use of human stem-cell-derived cultures, organoids and animal models to inform the HDCA, especially for prenatal tissues that are hard to acquire. Finally, we provide a roadmap towards a complete atlas of human development. This Perspective outlines the Human Developmental Cell Atlas initiative, which uses state-of-the-art technologies to map and model human development across gestation, and discusses the early milestones that have been achieved.read more
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Diagnostic potential of the amniotic fluid cells transcriptome in deciphering mendelian disease: a proof-of-concept
Mianne Lee,Anna Ka-Yee Kwong,M. M. Chui,Jeffrey Fong Ting Chau,Christopher C.Y. Mak,Sandy Leung-Kuen Au,H. Lo,Kelvin Y.K. Chan,Vicente A. Yépez,Julien Gagneur,Anita Sik Yau Kan,Brian H.Y. Chung +11 more
TL;DR: In this paper , the expression of clinically relevant genes in amniotic fluid (AF) cells compared with whole blood and fibroblasts was examined and it was shown that the number of well-expressed genes in AF cells was comparable to that in fibro-blasts and much higher than that in blood across different disease categories.
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New insights empowered by single-cell sequencing: From neural crest to enteric nervous system
Zhixin Li,Elly Sau-Wai Ngan +1 more
TL;DR: The use of single-cell multimodal sequencing to explore the chromatin accessibility, gene expression and spatial transcriptome has propelled the success in untangling the unknowns in the enteric nervous system (ENS) and provided unprecedented resources for building new diagnostic framework for enteric neuropathies as mentioned in this paper .
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Spatiotemporal transcriptome atlas of human embryos after gastrulation
Jiexue Pan,Yuejiao Li,Zhongliang Lin,Qing Lan,Huixi Chen,Yun-Hui Tang,Yue Xu,Yiting Mao,Qinfang Chen,Yichun Guan,N. N. Meng,Xiangjuan Li,Xiaoying Yao,Qiuyu Qin,Meiqi Luo,Xin Jin,Jian-Zhong Sheng,Congjian Xu,Xin-Mei Liu,Yanting Wu,Chenming Xu,Lijian Zhao,Hongbo Yang,Ya Gao,Guo-Lian Ding,Xun Xu,HeFeng Huang +26 more
TL;DR: In this paper , a spatiotemporal transcriptome atlas of gene expression in the human embryo at early gestation is presented, which is critical in understanding embryo development, organogenesis, and disease origins.
Journal ArticleDOI
Changes in and asymmetry of the proteome in the human fetal frontal lobe during early development
Xiaotian Zhao,Wen-Jia Liang,Wenjun Wang,Hailang Liu,Xiaolei Zhang,C. Liu,Caiting Zhu,Baoxia Cui,Yuchun Tang,Shuwei Liu +9 more
TL;DR: In this paper , the authors analyzed developmental changes in and asymmetry of the proteome in the bilateral frontal lobes of three foetal specimens in the late first trimester of pregnancy.
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Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC
Songlei Liu,Li Li,Fan Zhang,Björn van Sambeek,Evan Appleton,Alex H. M. Ng,Parastoo Khoshakhlagh,Yuting Chen,Mariana Garcia-Corral,Chun-Ting Wu,Jeremy Y. Huang,Yuqi Tan,George Chao,John Aach,Jenny M. Tam,Elaine T. Lim,Soumya Raychaudhuri,George M. Church +17 more
TL;DR: An iterative, pooled single-cell TF screening method is developed that improves the identification of effective TF combinations using the generation of human microglia-like cells as a testbed and describes what will be needed to fashion these methods into a generalized integrated pipeline, further ideas for enhancement, and possible applications.
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Heidi S. Phillips,Samir Kharbanda,Ruihuan Chen,William F. Forrest,Robert Soriano,Thomas D. Wu,Anjan Misra,Janice Nigro,Howard Colman,Liliana Soroceanu,P. Mickey Williams,Zora Modrusan,Burt G. Feuerstein,Kenneth Aldape +13 more
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SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes.
Waradon Sungnak,Ni Huang,Christophe Bécavin,Marijn Berg,Rachel Queen,Monika Litvinukova,Monika Litvinukova,Carlos Talavera-López,Henrike Maatz,Daniel Reichart,Fotios Sampaziotis,Kaylee B Worlock,Masahiro Yoshida,Josephine Barnes +13 more
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