Journal ArticleDOI
Characterization of protective extracellular membrane-derived vesicles produced by Streptococcus pneumoniae
Alfonso Olaya-Abril,Rafael Prados-Rosales,Michael J. McConnell,Reyes Martín-Peña,José A. González-Reyes,Irene Jiménez-Munguía,Lidia Gómez-Gascón,Javier Burón Fernández,Jose L. Luque-Garcia,Carlos García-Lidón,Hector Estevez,Jerónimo Pachón,Ignacio Obando,Arturo Casadevall,Liise Anne Pirofski,Manuel J. Rodríguez-Ortega,Manuel J. Rodríguez-Ortega +16 more
TLDR
This work demonstrates the active production of vesicle production by Streptococcus pneumoniae from the plasma membrane, rather than being a product from cell lysis, and shows that pneumococcus produces membrane-derived vesicles particularly enriched in lipoproteins and short-chain fatty acids.About:
This article is published in Journal of Proteomics.The article was published on 2014-06-25. It has received 172 citations till now. The article focuses on the topics: Secretion & Vesicle.read more
Citations
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Journal ArticleDOI
Applying extracellular vesicles based therapeutics in clinical trials - an ISEV position paper
Thomas Lener,Mario Gimona,Ludwig Aigner,Verena Börger,Edit I. Buzás,Giovanni Camussi,Nathalie Chaput,Devasis Chatterjee,Felipe A. Court,Hernando A. del Portillo,Lorraine O'Driscoll,Stefano Fais,Juan M. Falcón-Pérez,Ursula Felderhoff-Mueser,Lorenzo Fraile,Yong Song Gho,André Görgens,Ramesh C. Gupta,An Hendrix,Dirk M. Hermann,Andrew F. Hill,Fred H. Hochberg,Peter A. Horn,Dominique P.V. de Kleijn,Lambros Kordelas,Boris W. Kramer,Eva-Maria Krämer-Albers,Sandra Laner-Plamberger,Saara Laitinen,Tommaso Leonardi,Magdalena J. Lorenowicz,Sai Kiang Lim,Jan Lötvall,Casey A. Maguire,Antonio Marcilla,Irina Nazarenko,Takahiro Ochiya,Tushar Patel,Shona Pedersen,Gabriella Pocsfalvi,Stefano Pluchino,Peter J. Quesenberry,Ilona G. Reischl,Francisco J. Rivera,Ralf Sanzenbacher,Katharina Schallmoser,Ineke Slaper-Cortenbach,Dirk Strunk,Torsten Tonn,Pieter Vader,Pieter Vader,Bas W.M. van Balkom,Marca H. M. Wauben,Samir El Andaloussi,Samir El Andaloussi,Clotilde Théry,Eva Rohde,Bernd Giebel +57 more
TL;DR: In this paper, the authors summarize recent developments and the current knowledge of extracellular vesicles (EVs) and discuss safety and regulatory requirements that must be considered for pharmaceutical manufacturing and clinical application.
Journal ArticleDOI
Through the wall: extracellular vesicles in Gram-positive bacteria, mycobacteria and fungi
TL;DR: The current status of vesiculogenesis research in thick-walled microorganisms is described and the cargo and functions associated with EVs in these species are discussed.
Journal ArticleDOI
Gram-negative and Gram-positive bacterial extracellular vesicles
TL;DR: This review provides an overview of the current understanding on Gram-negative and Gram-positive bacterial extracellular vesicles, especially regarding the biogenesis, components, and functions in poly-species communities.
Journal ArticleDOI
Extracellular membrane vesicles in the three domains of life and beyond.
TL;DR: This review of current knowledge on EVs in the three domains of life and their interactions with the viral world concludes that the regulation of lipid composition plays a major role in initiating membrane curvature.
Journal ArticleDOI
Modern isolation and separation techniques for extracellular vesicles.
TL;DR: This review focuses on recently emerging and modern EV isolation and separation techniques, including size-, charge-, and affinity- based techniques while excluding ultracentrifugation and precipitation-based techniques due to their multiple limitations.
References
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Journal ArticleDOI
Standardization of sample collection, isolation and analysis methods in extracellular vesicle research
Kenneth W. Witwer,Edit I. Buzás,Lynne T. Bemis,Adriana Bora,Cecilia Lässer,Jan Lötvall,Esther Nolte-‘t Hoen,Melissa G. Piper,Sarada Sivaraman,Johan Skog,Clotilde Théry,Marca H. M. Wauben,Fred H. Hochberg +12 more
TL;DR: The need for standardization of specimen handling, appropriate normative controls, and isolation and analysis techniques to facilitate comparison of results is emphasized, and it is recognized that continual development and evaluation of techniques will be necessary as new knowledge is amassed.
Journal ArticleDOI
Sustained Reductions in Invasive Pneumococcal Disease in the Era of Conjugate Vaccine
Tamara Pilishvili,Catherine Lexau,Monica M. Farley,Monica M. Farley,James L. Hadler,Lee H. Harrison,Nancy M. Bennett,Arthur Reingold,Ann Thomas,William Schaffner,Allen S. Craig,Philip J. Smith,Bernard Beall,Cynthia G. Whitney,Matthew R. Moore +14 more
TL;DR: Dramatic reductions in IPD after PCV7 introduction in the United States remain evident 7 years later, and IPD rates caused by serotype 19A and other non-PCV7 types have increased but remain low relative to decreases in PCV 7-type IPD.
Journal ArticleDOI
Serotype replacement in disease after pneumococcal vaccination
TL;DR: The magnitude of serotype replacement in disease can be attributed, in part, to a combination of lower invasiveness of the replacing serotypes, biases in the pre-vaccine carriage data (unmasking), and bias in the disease surveillance systems that could underestimate the true amount of replacement.
Journal ArticleDOI
Effect of Introduction of the Pneumococcal Conjugate Vaccine on Drug-Resistant Streptococcus pneumoniae
Moe H. Kyaw,Ruth Lynfield,William Schaffner,Allen S. Craig,James L. Hadler,Arthur Reingold,Ann Thomas,Lee H. Harrison,Nancy M. Bennett,Monica M. Farley,Monica M. Farley,Richard R. Facklam,James H. Jorgensen,John M. Besser,Elizabeth R. Zell,Anne Schuchat,Cynthia G. Whitney +16 more
TL;DR: The rate of antibiotic-resistant invasive pneumococcal infections decreased in young children and older persons after the introduction of the conjugate vaccine, and there was an increase in infections caused by serotypes not included in the vaccine.
Journal ArticleDOI
Genome of the bacterium Streptococcus pneumoniae strain R6.
JoAnn Hoskins,William E. Alborn,Jeffrey S. Arnold,Larry C. Blaszczak,Stanley Gene Burgett,Bradley S. DeHoff,Shawn T. Estrem,Lori Fritz,Dong-Jing Fu,Wendy Fuller,Chad D. Geringer,Raymond Gilmour,Jennifer S. Glass,Hamid Khoja,Angelika R. Kraft,Robert E. Lagace,Donald J. LeBlanc,Linda N. Lee,Elliot J. Lefkowitz,Jin Lu,Patti Matsushima,Scott M. McAhren,Margaret A. Mchenney,Kevin McLeaster,Christopher W. Mundy,Thalia I. Nicas,Franklin H. Norris,MaryJeanne O'Gara,Robert B. Peery,Gregory T. Robertson,Pamela Kay Rockey,Pei-Ming Sun,Malcolm E. Winkler,Yong Yang,Michelle Young-Bellido,Genshi Zhao,Christopher A. Zook,Richard H. Baltz,S. Richard Jaskunas,Paul Robert Rosteck,Paul L. Skatrud,John I. Glass +41 more
TL;DR: In the analysis of the genome, a large number of new uncharacterized genes predicted to encode proteins that either reside on the surface of the cell or are secreted are identified and there may be new targets for vaccine and antibiotic development.