ER-to-Golgi trafficking of procollagen in the absence of large carriers
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TLDR
Analysis of endogenous procollagen and a new engineered GFP-tagged form shows that transport to the Golgi occurs in the absence of large (>350 nm) carriers, and proposes a short-loop model of COPII-dependent ER-to-Golgi traffic that does not invoke long-range trafficking of large vesicular structures.Abstract:
Secretion and assembly of collagen are fundamental to the function of the extracellular matrix. Defects in the assembly of a collagen matrix lead to pathologies including fibrosis and osteogenesis imperfecta. Owing to the size of fibril-forming procollagen molecules it is assumed that they are transported from the endoplasmic reticulum to the Golgi in specialized large COPII-dependent carriers. Here, analyzing endogenous procollagen and a new engineered GFP-tagged form, we show that transport to the Golgi occurs in the absence of large (>350 nm) carriers. Large GFP-positive structures were observed occasionally, but these were nondynamic, are not COPII positive, and are labeled with markers of the ER. We propose a short-loop model of COPII-dependent ER-to-Golgi traffic that, while consistent with models of ERGIC-dependent expansion of COPII carriers, does not invoke long-range trafficking of large vesicular structures. Our findings provide an important insight into the process of procollagen trafficking and reveal a short-loop pathway from the ER to the Golgi, without the use of large carriers.read more
Citations
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ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER.
Aubrey V. Weigel,Chi-Lun Chang,Gleb Shtengel,C. Shan Xu,David P. Hoffman,Melanie Freeman,Melanie Freeman,Nirmala Iyer,Jesse Aaron,Satya Khuon,John A. Bogovic,Wei Qiu,Harald F. Hess,Jennifer Lippincott-Schwartz +13 more
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COPII-mediated trafficking at the ER/ERGIC interface.
TL;DR: Recent findings that highlight the roles of COPII and its regulators in the biogenesis of tubular COPII‐coated carriers in mammalian cells that enable cargo transport between the endoplasmic reticulum and ER‐Golgi intermediate compartments are reviewed.
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Coatopathies: Genetic Disorders of Protein Coats.
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Mechanics and structural stability of the collagen triple helix
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ER-to-Golgi Transport: A Sizeable Problem
TL;DR: Current models describing the dynamics and mechanisms of ER-Golgi transport are discussed, challenging long-held models of vesicular transport of large matrix proteins and are implicating less well-defined carriers and direct interconnections between organelles.
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