Evaluation of recombinase-based isothermal amplification assays for point-of-need detection of SARS-CoV-2 in resource-limited settings.
Prakash Ghosh,Rajashree Chowdhury,Mohammad Enayet Hossain,Faria Hossain,Mojnu Miah,Md. Utba Rashid,James Baker,Mohammed Ziaur Rahman,Mustafizur Rahman,Xuejun Ma,Malcolm S. Duthie,Ahmed Abd El Wahed,Dinesh Mondal +12 more
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TLDR
In this article, two recombinase-based isothermal techniques, reverse transcription recombinases polymerase amplification (RT-RPA) and reverse transcription aided amplification (RAA), were evaluated for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in clinical samples.About:
This article is published in International Journal of Infectious Diseases.The article was published on 2022-01-01 and is currently open access. It has received 9 citations till now. The article focuses on the topics: Recombinase Polymerase Amplification & Loop-mediated isothermal amplification.read more
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Simplified Cas13-based assays for the fast identification of SARS-CoV-2 and its variants
Jon Arizti-Sanz,A’Doriann Bradley,Yibin B. Zhang,Chloe K. Boehm,Catherine A. Freije,M. Grunberg,Tinna Solveig F. Kosoko-Thoroddsen,Nicole L. Welch,Priya P. Pillai,Sreekar Mantena,Gae-Hwan Kim,Jessica N. Uwanibe,O. G. John,Philomena Eromon,Gregory Kocher,Robin Gross,Justin S. Lee,Lisa E. Hensley,Bronwyn L. MacInnis,Jeremy Johnson,Michael Springer,Christian T. Happi,Pardis C. Sabeti,Cameron Myhrvold +23 more
TL;DR: ShINEv.2 as discussed by the authors detects SARS-CoV-2 in nasopharyngeal samples with 90.5% sensitivity and 100% specificity in less than 90 min, using lateral flow technology and incubation in a heat block at 37 °C.
Journal ArticleDOI
Development of a multi‐recombinase polymerase amplification assay for rapid identification of COVID‐19, influenza A and B
Liguo Liang,Miaojin Zhu,Rui He,Danrong Shi,Rui Luo,Jia Ji,Liang Cheng,Xiangyun Lu,Wei Lu,Fu-Ming Liu,Nanping Wu,Hang Chen,Zhe Chen,Hangping Yao +13 more
TL;DR: A recombinase polymerase amplification (RPA) method for simple and rapid amplification of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2), the causative agent of COVID‐19 and Influenza A (H1N1, H3N2) and B (influenza B) is developed.
Journal ArticleDOI
Recent progress in diagnosis and treatment of Human African Trypanosomiasis has made the elimination of this disease a realistic target by 2030
TL;DR: In this article , the authors proposed a CRISPR-Cas technology to improve the intrinsic diagnostic characteristics of molecular approaches, which will become crucial in the near future, as preventing the resurgence of HAT will be a priority and will require tools with extreme high positive and negative predicted values.
Journal ArticleDOI
Multiple ligation–Assisted recombinase polymerase amplification for highly sensitive and selective colorimetric detection of SARS-CoV-2
TL;DR: In this paper , a new method for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), targeting a specific region “N gene, was presented.
Journal ArticleDOI
Validación clínica de la prueba RT-LAMP para el diagnóstico rápido del SARS-CoV-2
Leidy Viviana Muñoz Hurtado,Diana Ximena Herrera Díaz,Karen Garcia Escorcia,Laura Daniela Niño Flórez,Yesit Bello,Yirys Díaz,Elkin Navarro,Leonardo C. Pacheco,Nataly Galán,Roger Gustems Maestre,Antonio Mejías Acosta,Lisandro A. Pacheco +11 more
TL;DR: The RT-LAMP colorimétrica as mentioned in this paper was used to detect the gen N del SARS-CoV-2 mediante un set of cebadores previamente reportados (set de Broughton), arrojó valores de 0,97 (0,85-1,00) and 0,81 (0-65-0,92), respectivamente, with un intervalo de confianza del 95%.
References
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Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
Chaolin Huang,Yeming Wang,Xingwang Li,Lili Ren,Jianping Zhao,Yi Hu,Li Zhang,Guohui Fan,Jiuyang Xu,Xiaoying Gu,Zhenshun Cheng,Ting Yu,Jia'an Xia,Yuan Wei,Wenjuan Wu,Xuelei Xie,Wen Yin,Li Hui,Min Liu,Yan Xiao,Hong Gao,Li Guo,Jungang Xie,Guang-Fa Wang,Rongmeng Jiang,Zhancheng Gao,Qi Jin,Jianwei Wang,Bin Cao +28 more
TL;DR: The epidemiological, clinical, laboratory, and radiological characteristics and treatment and clinical outcomes of patients with laboratory-confirmed 2019-nCoV infection in Wuhan, China, were reported.
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Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study
Nanshan Chen,Min Zhou,Xuan Dong,Jie-Ming Qu,Fengyun Gong,Yang Han,Yang Qiu,Jingli Wang,Ying Liu,Yuan Wei,Jia'an Xia,Ting Yu,Xinxin Zhang,Li Zhang +13 more
TL;DR: Characteristics of patients who died were in line with the MuLBSTA score, an early warning model for predicting mortality in viral pneumonia, and further investigation is needed to explore the applicability of the Mu LBSTA scores in predicting the risk of mortality in 2019-nCoV infection.
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Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR.
Victor M. Corman,Olfert Landt,Marco Kaiser,Richard Molenkamp,Adam Meijer,Daniel K.W. Chu,Tobias Bleicker,Sebastian Brünink,Julia Schneider,Marie Luisa Schmidt,Daphne G.J.C. Mulders,Bart L. Haagmans,Bas van der Veer,Sharon van den Brink,Lisa Wijsman,Gabriel Goderski,Jean Louis Romette,Joanna Ellis,Maria Zambon,Malik Peiris,Herman Goossens,Chantal B.E.M. Reusken,Marion Koopmans,Christian Drosten +23 more
TL;DR: A validated diagnostic workflow for 2019-nCoV is presented, its design relying on close genetic relatedness of 2019- nCoV with SARS coronavirus, making use of synthetic nucleic acid technology.
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COVID-19: Transmission, prevention, and potential therapeutic opportunities.
TL;DR: Current therapeutic options, preventive methods and transmission routes of COVID-19 are discussed and infection prevention, early viral detection and identification of successful treatment protocols provide the best approach in controlling disease spread.
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Diagnostics for SARS-CoV-2 infections.
Bhavesh D. Kevadiya,Jatin Machhi,Jonathan Herskovitz,Maxim D. Oleynikov,Wilson R. Blomberg,Neha Bajwa,Dhruvkumar Soni,Srijanee Das,Mahmudul Hasan,Milankumar Patel,Ahmed M. Senan,Santhi Gorantla,JoEllyn M McMillan,Benson J Edagwa,Robert Eisenberg,Channabasavaiah B. Gurumurthy,St Patrick Reid,Chamindie Punyadeera,Linda Chang,Howard E. Gendelman +19 more
TL;DR: A review of available and in-development diagnostic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) including nanomaterial-based tools is presented in this paper.
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