Induced Pluripotent Stem Cells and Embryonic Stem Cells Are Distinguished by Gene Expression Signatures
Mark H. Chin,Michael Mason,Wei Xie,Stefano Volinia,Michael A. Singer,Cory Peterson,G. Ambartsumyan,Otaren Aimiuwu,Laura Richter,Jin Zhang,Ivan Khvorostov,Vanessa Ott,Michael Grunstein,Neta Lavon,Nissim Benvenisty,Carlo M. Croce,Amander T. Clark,Tim Baxter,April D. Pyle,Michael A. Teitell,Matteo Pelegrini,Kathrin Plath,William E. Lowry +22 more
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TLDR
Genome-wide data and high-resolution array profiling demonstrated that there is no common specific subkaryotypic alteration that is required for reprograming and that reprogramming does not lead to genomic instability, and suggest that iPSCs should be considered a unique subtype of pluripotent cell.About:
This article is published in Cell Stem Cell.The article was published on 2009-07-02 and is currently open access. It has received 1036 citations till now. The article focuses on the topics: Induced pluripotent stem cell & Reprogramming.read more
Citations
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Epigenetic memory in induced pluripotent stem cells
Kibem Kim,Akiko Doi,Bo Wen,K. Ng,Rui Zhao,Patrick Cahan,Jonghwan Kim,Martin J. Aryee,Hong Ji,Lauren I.R. Ehrlich,Lauren I.R. Ehrlich,A. Yabuuchi,A. Takeuchi,K. C. Cunniff,H. Hongguang,Shannon McKinney-Freeman,Olaia Naveiras,T. J. Yoon,T. J. Yoon,Rafael A. Irizarry,Namyoung Jung,Jun Seita,Jacob H. Hanna,Peter Murakami,Rudolf Jaenisch,Ralph Weissleder,Stuart H. Orkin,Irving L. Weissman,Andrew P. Feinberg,George Q. Daley +29 more
TL;DR: It is observed that low-passage induced pluripotent stem cells (iPSCs) derived by factor-based reprogramming of adult murine tissues harbour residual DNA methylation signatures characteristic of their somatic tissue of origin, which favours their differentiation along lineages related to the donor cell, while restricting alternative cell fates.
Epigenetic memory in induced pluripotent stem cells
Kibem Kim,Akiko Doi,Bo Wen,K. Ng,Rui Zhao,Patrick Cahan,Jonghwan Kim,Martin J. Aryee,Hong Ji,Lauren I.R. Ehrlich,Lauren I.R. Ehrlich,A. Yabuuchi,A. Takeuchi,K. C. Cunniff,H. Hongguang,Shannon McKinney-Freeman,Olaia Naveiras,T. J. Yoon,T. J. Yoon,Rafael A. Irizarry,Namyoung Jung,Jun Seita,Jacob H. Hanna,Peter Murakami,Rudolf Jaenisch,Ralph Weissleder,Stuart H. Orkin,Irving L. Weissman,Andrew P. Feinberg,George Q. Daley +29 more
TL;DR: The authors showed that low-passage induced pluripotent stem cells (iPSCs) derived by factor-based reprogramming of adult murine tissues harbor residual DNA methylation signatures characteristic of their somatic tissue of origin.
Journal ArticleDOI
Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells
Ryan Lister,Mattia Pelizzola,Yasuyuki S. Kida,R. David Hawkins,Joseph R. Nery,Gary C. Hon,Jessica Antosiewicz-Bourget,Jessica Antosiewicz-Bourget,Ronan C. O'Malley,Rosa Castanon,Sarit Klugman,Michael Downes,Ruth T. Yu,Ron Stewart,Ron Stewart,Bing Ren,Bing Ren,James A. Thomson,Ronald M. Evans,Joseph R. Ecker +19 more
TL;DR: The first whole-genome profiles of DNA methylation at single-base resolution in five human iPSC lines, along with methylomes of ES cells, somatic cells, and differentiated iPSCs and ES cells are reported.
Book
Immunogenicity of Induced Pluripotent Stem Cells
TL;DR: Findings indicate that, in contrast to derivatives of ESCs, abnormal gene expression in some cells differentiated from iPSCs can induce T-cell-dependent immune response in syngeneic recipients.
Journal ArticleDOI
Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells
Jose M. Polo,Susanna Liu,Maria E. Figueroa,Warakorn Kulalert,Sarah Eminli,Kah Yong Tan,Effie Apostolou,Matthias Stadtfeld,Yushan Li,Toshihiro Shioda,Sridaran Natesan,Amy J. Wagers,Ari Melnick,Todd Evans,Konrad Hochedlinger +14 more
TL;DR: It is shown that iPSCs obtained from mouse fibroblasts, hematopoietic and myogenic cells exhibit distinct transcriptional and epigenetic patterns, and it is demonstrated that cellular origin influences the in vitro differentiation potentials of iPSC into embryoid bodies and different hematopsic cell types.
References
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Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: It is demonstrated that iPS cells can be generated from adult human fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc.
Journal ArticleDOI
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Junying Yu,Maxim A. Vodyanik,Kim Smuga-Otto,Jessica Antosiewicz-Bourget,Jennifer L. Frane,Shulan Tian,Jeff Nie,Gudrun A. Jonsdottir,Victor Ruotti,Ron Stewart,Igor I. Slukvin,James A. Thomson +11 more
TL;DR: This article showed that OCT4, SOX2, NANOG, and LIN28 factors are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells.
Journal ArticleDOI
Generation of germline-competent induced pluripotent stem cells
TL;DR: iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.
Journal ArticleDOI
Induction of Pluripotent Stem Cells From Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
TL;DR: This work generated induced pluripotent stem cells capable of germline transmission from murine somatic cells by transd, and demonstrated the ability of these cells to reprogram into patient-specific and disease-specific stem cells.
Related Papers (5)
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
Epigenetic memory in induced pluripotent stem cells
Kibem Kim,Akiko Doi,Bo Wen,K. Ng,Rui Zhao,Patrick Cahan,Jonghwan Kim,Martin J. Aryee,Hong Ji,Lauren I.R. Ehrlich,Lauren I.R. Ehrlich,A. Yabuuchi,A. Takeuchi,K. C. Cunniff,H. Hongguang,Shannon McKinney-Freeman,Olaia Naveiras,T. J. Yoon,T. J. Yoon,Rafael A. Irizarry,Namyoung Jung,Jun Seita,Jacob H. Hanna,Peter Murakami,Rudolf Jaenisch,Ralph Weissleder,Stuart H. Orkin,Irving L. Weissman,Andrew P. Feinberg,George Q. Daley +29 more