Installation of O-glycan sulfation capacities in human HEK293 cells for display of sulfated mucins
Yoshiki Narimatsu,Henrik Clausen +1 more
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In this paper , a cell-based O-glycan array with sulfation capacities was shown to be useful for display and production of glycoconjugates with sulfated o-glycans.About:
This article is published in Journal of Biological Chemistry.The article was published on 2022-02-01 and is currently open access. It has received 5 citations till now. The article focuses on the topics: Sulfation & Sulfotransferase.read more
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Recombinant mucin biotechnology and engineering.
TL;DR: Mucins represent a largely untapped class of polymeric building blocks for biomaterials, therapeutics, and other biotechnology as mentioned in this paper , and the opportunities for engineered mucins in biomedical applications ranging from in vitro models to therapeutics.
Journal ArticleDOI
Mechanistic and Structural Insights into the Specificity and Biological Functions of Bacterial Sulfoglycosidases
Zhen Zhang,Mochen Dong,Rémi Zallot,George L. Blackburn,Chengjian Wang,Long Chen,Patrick Baumann,Zuyan Wu,Zhongfu Wang,Haiming Fan,Christian Roth,Yi Jin,Yuan Long He +12 more
TL;DR: In this article , the catalytically important amino acids directly involved in the recognition and cleavage of 6S-GlcNAc, but not of 6-phospho-glc NAc, were revealed.
Journal ArticleDOI
Deep learning explains the biology of branched glycans from single-cell sequencing data
TL;DR: In this paper , a deep learning model was used to predict the glycan phenotypes of cells (mouse T lymphocytes) from transcripts, with the example of predicting β1,6GlcNAc-branching across T cell subtypes.
Journal ArticleDOI
Protocol Considerations for In Vitro Metabolic Glycoengineering of Non‐Natural Glycans
TL;DR: In this paper , a general approach for analog selection and protocols to ensure safe and efficacious analog usage by cells is described, and flow cytometry is used to quantify analog incorporation and set the stage for numerous follow-up applications.
Journal ArticleDOI
3′-sulfated LewisA/C: An oncofetal epitope associated with metaplastic and oncogenic plasticity of the gastrointestinal foregut
Koushik K. Das,Jeffrey W. Brown +1 more
TL;DR: In this article , a clinically validated biomarker for high-risk metaplasia and cancer throughout the gastrointestinal foregut: esophagus, stomach, and pancreas was explored.
References
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The PRIDE database and related tools and resources in 2019: improving support for quantification data.
Yasset Perez-Riverol,Attila Csordas,Jingwen Bai,Manuel Bernal-Llinares,Suresh Hewapathirana,Deepti J. Kundu,Avinash Inuganti,Johannes Griss,Johannes Griss,Gerhard Mayer,Martin Eisenacher,Enrique Perez,Julian Uszkoreit,Julianus Pfeuffer,Timo Sachsenberg,Şule Yılmaz,Shivani Tiwary,Juergen Cox,Enrique Audain,Mathias Walzer,Andrew F. Jarnuczak,Tobias Ternent,Alvis Brazma,Juan Antonio Vizcaíno +23 more
TL;DR: Key statistics on the current data contents and volume of downloads are outlined, and how PRIDE data are starting to be disseminated to added-value resources including Ensembl, UniProt and Expression Atlas are outlined.
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Order out of chaos: assembly of ligand binding sites in heparan sulfate.
Jeffrey D. Esko,Scott B. Selleck +1 more
TL;DR: A review of recent developments in the biosynthetic apparatus of metazoan organisms provides a resource for investigators interested in the incredible diversity and specificity of this process.
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Syndecan–syntenin–ALIX regulates the biogenesis of exosomes
Maria Francesca Baietti,Zhe Zhang,Zhe Zhang,Eva Mortier,Aurélie Melchior,Gisèle Degeest,Annelies Geeraerts,Ylva Ivarsson,Fabienne Depoortere,Christien Coomans,Elke Vermeiren,Pascale Zimmermann,Guido David +12 more
TL;DR: Evidence is reported that the syndecan heparan sulphate proteoglycans and their cytoplasmic adaptor syntenin control the formation of exosomes, indicating a key role for syndecans–syntenin–ALIX in membrane transport and signalling processes.
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Precision mapping of the human O-GalNAc glycoproteome through SimpleCell technology
Catharina Steentoft,Sergey Y. Vakhrushev,Hiren J. Joshi,Hiren J. Joshi,Yun Kong,Malene Bech Vester-Christensen,Katrine T. Schjoldager,Kirstine Lavrsen,Sally Dabelsteen,Nis Borbye Pedersen,Lara Marcos-Silva,Lara Marcos-Silva,Ramneek Gupta,Eric P. Bennett,Ulla Mandel,Søren Brunak,Søren Brunak,Hans H. Wandall,Steven B. Levery,Henrik Clausen +19 more
TL;DR: A genetic engineering approach using human cell lines to simplify O‐glycosylation (SimpleCells) that enables proteome‐wide discovery of O-glycan sites using ‘bottom‐up’ ETD‐based mass spectrometric analysis and an improved NetOGlyc4.0 model for prediction of O‐ Glycoproteins.
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A novel role for 3-O-sulfated heparan sulfate in herpes simplex virus 1 entry
Deepak Shukla,Jian Liu,Peter Blaiklock,Nicholas W. Shworak,Nicholas W. Shworak,Xiaomei Bai,Jeffrey D. Esko,Gary H. Cohen,Roselyn J. Eisenberg,Robert D. Rosenberg,Robert D. Rosenberg,Patricia G. Spear +11 more
TL;DR: It is shown that heparan sulfate modified by a subset of the multiple D-glucosaminyl 3-O-sulfotransferase isoforms provides sites for the binding of a third viral glycoprotein, gD, and for initiation of HSV-1 entry.
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