Journal ArticleDOI
Knockdown of cyclin D1 inhibits proliferation, induces apoptosis, and attenuates the invasive capacity of human glioblastoma cells
Junyu Wang,Qi Wang,Yong Cui,Zhen Yang Liu,Wei Zhao,Chun Lin Wang,Yan Dong,Lijun Hou,Guohan Hu,Chun Luo,Juxiang Chen,Yicheng Lu +11 more
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TLDR
Overexpression of cyclin D1 enhanced the proliferation and invasive potential of human glioblastoma cells, while reducing apoptosis, and may provide a new gene therapy approach for patients with malignant glioma.Abstract:
Elevated cyclin D1 (CCND1) in human glioblastoma correlates with poor clinical prognosis. In this study, the human glioblastoma cell lines SHG-44 and U251 were stably transfected with short hairpin RNA (shRNA) targeting cyclin D1 or with ectogenic cyclin D1 by lentivirus-mediated transfection. Glioblastoma cells overexpressing or underexpressing cyclin D1 were then examined by in vitro growth assays, apoptosis assays, cell cycle analysis, and invasion assays. Cyclin D1 knockdown in SHG-44 cells inhibited cell proliferation, induced apoptosis, and attenuated migration across Matrigel, a model of invasive capacity. Western blot analysis and quantitative reverse-transcription polymerase chain reaction (RT-PCR) revealed that cells underexpressing CCND1 exhibited decreased multidrug resistance protein 1 (MDR1) and B-cell lymphoma-2 (Bcl-2) expression, but enhanced apoptosis effector caspase-3 expression. In contrast, cyclin D1 overexpression promoted cell proliferation, attenuated apoptosis, and enhanced invasive capacity. Furthermore, cyclin D1 overexpression was associated with increased expression of MDR1 and Bcl-2, and decreased caspase-3 expression. Results using the U251 cell line confirmed the effects of CCND1-targeted shRNA and lentivirus-mediated overexpression on proliferation and apoptosis of glioblastoma cells. Overexpression of cyclin D1 enhanced the proliferation and invasive potential of human glioblastoma cells, while reducing apoptosis. The ability to suppress the malignant phenotype by downregulating cyclin D1 expression may provide a new gene therapy approach for patients with malignant glioma.read more
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Journal ArticleDOI
Genetic compensation: A phenomenon in search of mechanisms.
TL;DR: This review revisits studies reporting genetic compensation in higher eukaryotes and outlines possible molecular mechanisms, which may include both transcriptional and posttranscriptional processes.
Journal ArticleDOI
Nuclear factor-κB in glioblastoma: insights into regulators and targeted therapy.
TL;DR: The importance of this transcription factor in the overall malignant phenotype suggests that more rational and specific targeting of NF-κB-dependent pathways can make a significant contribution to the management of GBM.
Journal ArticleDOI
MicroRNA-195 Inhibits the Proliferation of Human Glioma Cells by Directly Targeting Cyclin D1 and Cyclin E1
TL;DR: It is suggested that miR-195 plays an important role to inhibit the proliferation of glioma cells, and present a novel mechanism for direct miRNA-mediated suppression of cyclin D1 and cyclin E1 inglioma.
Journal ArticleDOI
miR-340 inhibits glioblastoma cell proliferation by suppressing CDK6, cyclin-D1 and cyclin-D2.
TL;DR: Evidence is provided for a function of miR-340 in the inhibition of glioblastoma cell proliferation and for its arrest in the G0/G1 cell cycle phase using bioinformatics, luciferase assay and western blotting.
Journal ArticleDOI
MiR-15b targets cyclin D1 to regulate proliferation and apoptosis in glioma cells.
TL;DR: The findings identified that miR-15b may function as a glioma suppressor by targeting the Cyclin D1, which may provide a novel therapeutic strategy for treatment ofglioma.
References
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Journal Article
Cell cycle control in mammalian cells : role of cyclins, cyclin dependent kinases (CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors (CKIs)
Xavier Graña,Reddy Ep +1 more
TL;DR: In this review, the mechanisms by which the mammalian cell cycle engine integrates intracellular and extracellular signals of different nature are discussed.
Journal ArticleDOI
Mammary Hyperplasia and Carcinoma in MMTV-cyclin D1 Transgenic Mice
Timothy C. Wang,Robert D. Cardiff,Lawrence R. Zukerberg,Emma Lees,Andrew Arnold,Emmett V. Schmidt +5 more
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