Laboratory Diagnosis of COVID-19: Current Issues and Challenges.
TLDR
In the preanalytical stage, collecting the proper respiratory tract specimen at the right time from the right anatomic site is essential for a prompt and accurate molecular diagnosis of COVID-19, and real-time reverse transcription-PCR assays remain the molecular test of choice for the etiologic diagnosis of SARS-CoV-2 infection while antibody-based techniques are being introduced as supplemental tools.Abstract:
The COVID-19 outbreak has had a major impact on clinical microbiology laboratories in the past several months. This commentary covers current issues and challenges for the laboratory diagnosis of infections caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In the preanalytical stage, collecting the proper respiratory tract specimen at the right time from the right anatomic site is essential for a prompt and accurate molecular diagnosis of COVID-19. Appropriate measures are required to keep laboratory staff safe while producing reliable test results. In the analytic stage, real-time reverse transcription-PCR (RT-PCR) assays remain the molecular test of choice for the etiologic diagnosis of SARS-CoV-2 infection while antibody-based techniques are being introduced as supplemental tools. In the postanalytical stage, testing results should be carefully interpreted using both molecular and serological findings. Finally, random-access, integrated devices available at the point of care with scalable capacities will facilitate the rapid and accurate diagnosis and monitoring of SARS-CoV-2 infections and greatly assist in the control of this outbreak.read more
Citations
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Biosensor and molecular-based methods for the detection of human coronaviruses: A review.
Addisu Demeke Teklemariam,Manalee Samaddar,Mona G. Alharbi,Rashad R. Al-Hindi,Arun K. Bhunia +4 more
TL;DR: A short overview of recent advancements in molecular and biosensor-based diagnosis of viruses, including the human coronaviruses, is provided and the challenges and future perspectives of these detection technologies are highlighted.
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The diagnostic methods in the COVID-19 pandemic, today and in the future.
So Yat Wu,Hoi Shan Yau,Man Yee Yu,Hin Fung Tsang,Lawrence W. C. Chan,William C. Cho,Allen Chi-Shing Yu,Aldrin Kay-Yuen Yim,Marco Jing Woei Li,Yin Kwan Evelyn Wong,Xiao Meng Pei,Sze Chuen Cesar Wong +11 more
TL;DR: The knowledge about the causative pathogen of CO VID-19 and various diagnostic methods in this pandemic is summarized for better understanding of the limitations and the nuances of virus testing for COVID-19.
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Anosmia and olfactory tract neuropathy in a case of COVID-19.
Chia Wen Li,Ling Shan Syue,Yi Shan Tsai,Min Chi Li,Ching Lung Lo,Chin Shiang Tsai,Po Lin Chen,Wen Chien Ko,Nan Yao Lee +8 more
TL;DR: A previously healthy man of COVID-19 presenting with anosmia as the obvious symptom with relevant radiological findings on brain magnetic resonance imaging is reported.
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End-to-End AI-Based Point-of-Care Diagnosis System for Classifying Respiratory Illnesses and Early Detection of COVID-19
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Complex features extraction with deep learning model for the detection of COVID19 from CT scan images using ensemble based machine learning approach
TL;DR: In this paper , Contrast Limited Histogram Equalization (CLAHE) was applied to CT images as a preprocessing step for enhancing the quality of the images and a novel Convolutional Neural Network (CNN) model was developed to extract 100 prominent features from a total of 2482 CT scan images.
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