Laboratory Diagnosis of COVID-19: Current Issues and Challenges.
TLDR
In the preanalytical stage, collecting the proper respiratory tract specimen at the right time from the right anatomic site is essential for a prompt and accurate molecular diagnosis of COVID-19, and real-time reverse transcription-PCR assays remain the molecular test of choice for the etiologic diagnosis of SARS-CoV-2 infection while antibody-based techniques are being introduced as supplemental tools.Abstract:
The COVID-19 outbreak has had a major impact on clinical microbiology laboratories in the past several months. This commentary covers current issues and challenges for the laboratory diagnosis of infections caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In the preanalytical stage, collecting the proper respiratory tract specimen at the right time from the right anatomic site is essential for a prompt and accurate molecular diagnosis of COVID-19. Appropriate measures are required to keep laboratory staff safe while producing reliable test results. In the analytic stage, real-time reverse transcription-PCR (RT-PCR) assays remain the molecular test of choice for the etiologic diagnosis of SARS-CoV-2 infection while antibody-based techniques are being introduced as supplemental tools. In the postanalytical stage, testing results should be carefully interpreted using both molecular and serological findings. Finally, random-access, integrated devices available at the point of care with scalable capacities will facilitate the rapid and accurate diagnosis and monitoring of SARS-CoV-2 infections and greatly assist in the control of this outbreak.read more
Citations
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Antibody responses to SARS-CoV-2 in patients with COVID-19.
Quanxin Long,Bai Zhong Liu,Hai Jun Deng,Gui Cheng Wu,Kun Deng,Yao Kai Chen,Pu Liao,Jing Fu Qiu,Yong Lin,Xue Fei Cai,Deqiang Wang,Yuan Hu,Ji Hua Ren,Ni Tang,Yin Yin Xu,Li Hua Yu,Zhan Mo,Fang Gong,Xiao-li Zhang,Wen Guang Tian,Li Hu,Xian Xiang Zhang,Jiang Lin Xiang,Hong Xin Du,Hua Wen Liu,Chun Hui Lang,Xiao He Luo,Shao Bo Wu,Xiao Ping Cui,Zheng Zhou,Man Man Zhu,Jing Wang,Cheng Jun Xue,Xiao Feng Li,Li Wang,Zhi Jie Li,Kun Wang,Chang Chun Niu,Qing Jun Yang,Xiaojun Tang,Yong Zhang,Xia Mao Liu,Jin Jing Li,De Chun Zhang,Fan Zhang,Liu Ping,Jun Yuan,Qin Li,Jie Li Hu,Juan Chen,Ailong Huang +50 more
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A SARS-CoV-2 surrogate virus neutralization test based on antibody-mediated blockage of ACE2-spike protein-protein interaction.
Chee Wah Tan,Wan Ni Chia,Xijian Qin,Pei Liu,Mark I-Cheng Chen,Charles Tiu,Zhiliang Hu,Zhiliang Hu,Vivian Chih Wei Chen,Barnaby Edward Young,Barnaby Edward Young,Wan Rong Sia,Yee-Joo Tan,Yee-Joo Tan,Randy Foo,Yongxiang Yi,David C. Lye,Danielle E. Anderson,Lin-Fa Wang +18 more
TL;DR: A blocking assay based on the recombinant receptor-binding domain of SARS-CoV-2 spike protein and human angiotensin-converting enzyme 2 receptor provides an alternative to conventional antibody neutralization assays requiring live virus.
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Systematic review with meta-analysis of the accuracy of diagnostic tests for COVID-19.
Beatriz Böger,Mariana Millan Fachi,Raquel de Oliveira Vilhena,Alexandre de Fátima Cobre,Fernanda S. Tonin,Roberto Pontarolo +5 more
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TL;DR: A suite of complementary tests will inform crucial decisions by healthcare providers and policy makers and understanding their strengths and limitations will be critical to their judicious application for the development of algorithmic approaches to treatment and public health strategies.
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COVID-19: Coagulopathy, Risk of Thrombosis, and the Rationale for Anticoagulation.
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Detection of SARS-CoV-2 in Different Types of Clinical Specimens.
TL;DR: Results of PCR and viral RNA testing for SARS-CoV-2 in bronchoalveolar fluid, sputum, feces, blood, and urine specimens from patients with COVID-19 infection in China are described to identify possible means of non-respiratory transmission.
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