Journal ArticleDOI
Mass transfer and metabolic reactions in hepatocyte spheroids cultured in rotating wall gas-permeable membrane system
Efrem Curcio,Simona Salerno,Giuseppe Barbieri,Loredana De Bartolo,Enrico Drioli,Augustinus Bader +5 more
TLDR
The improved viability predicted by the model culturing hepatocyte spheroids in the RWMS, characterized by a higher O(2) permeability with respect to RWPS, was experimentally confirmed and demonstrated that the mathematical model used in this study represents a useful support to experimental procedures in order to obtain hepatocytespheroids with optimal size.About:
This article is published in Biomaterials.The article was published on 2007-12-01. It has received 228 citations till now. The article focuses on the topics: Limiting oxygen concentration & Spheroid.read more
Citations
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Journal ArticleDOI
Recent advances in three-dimensional multicellular spheroid culture for biomedical research
Ruei-Zhen Lin,Hwan-You Chang +1 more
TL;DR: The current understanding of multicellular spheroid formation mechanisms, their biomedical applications, and recent advances in sp heroid culture, manipulation, and analysis techniques are reviewed.
Journal ArticleDOI
Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME.
Patricio Godoy,Nicola J. Hewitt,Ute Albrecht,Melvin E. Andersen,Nariman Ansari,Sudin Bhattacharya,Johannes G. Bode,Jennifer Bolleyn,Christoph Borner,J Böttger,Albert Braeuning,Robert A. Budinsky,Britta Burkhardt,Neil R. Cameron,Giovanni Camussi,Chong Su Cho,Yun Jaie Choi,J. Craig Rowlands,Uta Dahmen,Georg Damm,Olaf Dirsch,María Teresa Donato,Jian Dong,Steven Dooley,Dirk Drasdo,Dirk Drasdo,Dirk Drasdo,Rowena Eakins,Karine Sá Ferreira,Valentina Fonsato,Joanna Fraczek,Rolf Gebhardt,Andrew Gibson,Matthias Glanemann,Christopher E. Goldring,María José Gómez-Lechón,Geny M. M. Groothuis,Lena Gustavsson,Christelle Guyot,David Hallifax,Seddik Hammad,Adam S. Hayward,Dieter Häussinger,Claus Hellerbrand,Philip Hewitt,Stefan Hoehme,Hermann-Georg Holzhütter,J. Brian Houston,Jens Hrach,Kiyomi Ito,Hartmut Jaeschke,Verena Keitel,Jens M. Kelm,B. Kevin Park,Claus Kordes,Gerd A. Kullak-Ublick,Edward L. LeCluyse,Peng Lu,Jennifer Luebke-Wheeler,Anna Lutz,Daniel J. Maltman,Madlen Matz-Soja,Patrick D. McMullen,Irmgard Merfort,Simon Messner,Christoph Meyer,Jessica Mwinyi,Dean J. Naisbitt,Andreas K. Nussler,Peter Olinga,Francesco Pampaloni,Jingbo Pi,Linda J. Pluta,Stefan Przyborski,Anup Ramachandran,Vera Rogiers,Cliff Rowe,Celine Schelcher,Kathrin Schmich,Michael Schwarz,Bijay Singh,Ernst H. K. Stelzer,Bruno Stieger,Regina Stöber,Yuichi Sugiyama,Ciro Tetta,Wolfgang E. Thasler,Tamara Vanhaecke,Mathieu Vinken,Thomas S. Weiss,Agata Widera,Courtney G. Woods,Jinghai James Xu,Kathy Yarborough,Jan G. Hengstler +94 more
TL;DR: This review encompasses the most important advances in liver functions and hepatotoxicity and analyzes which mechanisms can be studied in vitro and how closely hepatoma, stem cell and iPS cell–derived hepatocyte-like-cells resemble real hepatocytes.
Journal ArticleDOI
Advances in multicellular spheroids formation.
TL;DR: Among these methods, the approach in microfluidics with hydrogel support for MCS formation is promising because it allows essential cell–cell/cell–matrix interactions in a confined space.
Journal ArticleDOI
Human liver cell spheroids in extended perfusion bioreactor culture for repeated-dose drug testing.
Rui Tostoes,Sofia B. Leite,Margarida Serra,Janne Jensen,Petter Björquist,Manuel J.T. Carrondo,Catarina Brito,Paula M. Alves +7 more
TL;DR: Perfusion bioreactor cultures of primary human hepatocyte spheroids maintain a liver‐specific activity and architecture and are thus suitable for drug testing in a long‐term, repeated‐dose format.
Journal ArticleDOI
An oxygen-permeable spheroid culture system for the prevention of central hypoxia and necrosis of spheroids
TL;DR: Results indicate that this oxygen-permeable spheroid culture chip is useful for engineering 3D cellular constructs with high viability and functionality for tissue engineering.
References
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Book ChapterDOI
Preparation of isolated rat liver cells.
TL;DR: This chapter discusses preparation of isolated rat liver cells by incubation of rat liver minces with pronase, which results in most of the liver parenchyma is digested, while nonparenchymal cells remain intact and can be recovered from the incubate.
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Diffusion: Mass Transfer in Fluid Systems
TL;DR: An overview of diffusion and separation processes brings unsurpassed, engaging clarity to this complex topic as mentioned in this paper, which is a key part of the undergraduate chemical engineering curriculum and at the core of understanding chemical purification and reaction engineering.
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Control of hepatic gluconeogenesis through the transcriptional coactivator PGC-1.
J. Cliff Yoon,Pere Puigserver,Guoxun Chen,Jerry Donovan,Zhidan Wu,James Rhee,Guillaume Adelmant,John M. Stafford,C. Ronald Kahn,Daryl K. Granner,Christopher B. Newgard,Bruce M. Spiegelman +11 more
TL;DR: The results implicate PGC-1 as a key modulator of hepatic gluconeogenesis and as a central target of the insulin–cAMP axis in liver.
Journal ArticleDOI
Spheroidal aggregate culture of rat liver cells: histotypic reorganization, biomatrix deposition, and maintenance of functional activities.
TL;DR: It is concluded that cells dispersed as single cells from newborn rat liver conserve in part the necessary information to reconstruct a proper three-dimensional cyto-architecture and that the microenvironment so generated most likely represents a basic requirement for the optimal functioning of these differentiated cells.
Journal ArticleDOI
Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments.
Norio Koide,Khosaku Sakaguchi,Yayoi Koide,Kenichiro Asano,Mitsuhiko Kawaguchi,Hiroshi Matsushima,Tatsuro Takenami,Toshiyuki Shinji,Masaharu Mori,Takao Tsuji +9 more
TL;DR: Two distinct, mutually exclusive features of primary culture of adult hepatocytes apparently exist; monolayer culture with proliferative activity in an adherent environment and spheroid culture with poor proliferationative activity and high albumin-producing ability in a nonadherent environment.
Related Papers (5)
Recent advances in three-dimensional multicellular spheroid culture for biomedical research
Ruei-Zhen Lin,Hwan-You Chang +1 more