miR-409-3p/-5p promotes tumorigenesis, epithelial-to-mesenchymal transition, and bone metastasis of human prostate cancer.
Sajni Josson,Murali Gururajan,Peizhen Hu,Chen Shao,Gina Chia-Yi Chu,Haiyen E. Zhau,Chunyan Liu,Kaiqin Lao,Chia-Lun Lu,Yi-Tsung Lu,Jake Lichterman,Srinivas Nandana,Quanlin Li,Andre Rogatko,Dror Berel,Edwin M. Posadas,Ladan Fazli,Dhruv Sareen,Leland W.K. Chung +18 more
TLDR
Elevated expression of miR-409-3p/-5p was observed in bone metastatic prostate cancer cell lines and human prostate cancer tissues with higher Gleason scores and correlated with progression-free survival of patients with prostate cancer.Abstract:
Purpose: miR-409-3p/-5p is a miRNA expressed by embryonic stem cells, and its role in cancer biology and metastasis is unknown. Our pilot studies demonstrated elevated miR-409-3p/-5p expression in human prostate cancer bone metastatic cell lines; therefore, we defined the biologic impact of manipulation of miR-409-3p/-5p on prostate cancer progression and correlated the levels of its expression with clinical human prostate cancer bone metastatic specimens. Experimental Design: miRNA profiling of a prostate cancer bone metastatic epithelial-to-mesenchymal transition (EMT) cell line model was performed. A Gleason score human tissue array was probed for validation of specific miRNAs. In addition, genetic manipulation of miR-409-3p/-5p was performed to determine its role in tumor growth, EMT, and bone metastasis in mouse models. Results: Elevated expression of miR-409-3p/-5p was observed in bone metastatic prostate cancer cell lines and human prostate cancer tissues with higher Gleason scores. Elevated miR-409-3p expression levels correlated with progression-free survival of patients with prostate cancer. Orthotopic delivery of miR-409-3p/-5p in the murine prostate gland induced tumors where the tumors expressed EMT and stemness markers. Intracardiac inoculation (to mimic systemic dissemination) of miR-409-5p inhibitor–treated bone metastatic ARCaP M prostate cancer cells in mice led to decreased bone metastasis and increased survival compared with control vehicle–treated cells. Conclusion: miR-409-3p/-5p plays an important role in prostate cancer biology by facilitating tumor growth, EMT, and bone metastasis. This finding bears particular translational importance as miR-409-3p/-5p appears to be an attractive biomarker and/or possibly a therapeutic target to treat bone metastatic prostate cancer. Clin Cancer Res; 20(17); 4636–46. ©2014 AACR .read more
Citations
More filters
Journal ArticleDOI
Dimension reduction techniques for the integrative analysis of multi-omics data
Chen Meng,Oana A. Zeleznik,Gerhard G. Thallinger,Bernhard Kuster,Amin Moghaddas Gholami,Aedín C. Culhane +5 more
TL;DR: This work explores dimension reduction techniques as one of the emerging approaches for data integration, and how these can be applied to increase the understanding of biological systems in normal physiological function and disease.
Journal ArticleDOI
Regulation of prostate cancer progression by the tumor microenvironment.
TL;DR: The current data on the intricate relationship between inflammation, reactive stroma, tumor cells and disease progression in prostate cancer is reviewed.
Journal ArticleDOI
Unveiling massive numbers of cancer-related urinary-microRNA candidates via nanowires.
Takao Yasui,Takao Yasui,Takeshi Yanagida,Takeshi Yanagida,Satoru Ito,Yuki Konakade,Daiki Takeshita,Tsuyoshi Naganawa,Kazuki Nagashima,Taisuke Shimada,Noritada Kaji,Noritada Kaji,Yuta Nakamura,Ivan Adiyasa Thiodorus,Yong He,Sakon Rahong,Sakon Rahong,Masaki Kanai,Hiroshi Yukawa,Takahiro Ochiya,Tomoji Kawai,Yoshinobu Baba +21 more
TL;DR: The present device concept will provide a foundation for work toward the long-term goal of urine-based early diagnoses and medical checkups for cancer and identify urinary miRNAs that could potentially serve as biomarkers for cancer not only for urologic malignancies but also for nonurologic ones.
Journal ArticleDOI
Signaling pathways in cancer-associated fibroblasts and targeted therapy for cancer
Fanglong Wu,Jin Yang,Junjiang Liu,Ye Wang,Jingtian Mu,Qingxiang Zeng,Shuzhi Deng,Hongmei Zhou +7 more
TL;DR: In this paper, a comprehensive understanding of these signaling cascades in interactions between cancer cells and CAFs is necessary to fully realize the pivotal roles of CAFs in cancers, and three potential targeting strategies, including, epithelial-mesenchymal common targets, sequential target perturbation, and crosstalk-directed signaling targets, paving the way for CAF-directed or host cell-directed antitumor therapy.
Journal ArticleDOI
FABP4 as a key determinant of metastatic potential of ovarian cancer
Kshipra M. Gharpure,Sunila Pradeep,Sunila Pradeep,Marta Sans,Rajesha Rupaimoole,Cristina Ivan,Sherry Y. Wu,Emine Bayraktar,Archana S. Nagaraja,Lingegowda S. Mangala,Xinna Zhang,Monika Haemmerle,Wei Hu,Cristian Rodriguez-Aguayo,Michael McGuire,Celia Sze Ling Mak,Xiuhui Chen,Michelle A. Tran,Alejandro Villar-Prados,Guillermo N. Armaiz Pena,Ragini Kondetimmanahalli,Ryan Nini,Pranavi Koppula,Prahlad T. Ram,Jinsong Liu,Gabriel Lopez-Berestein,Keith A. Baggerly,Livia S. Eberlin,Anil K. Sood +28 more
TL;DR: In this article, the authors explored the underlying mechanisms that could increase metastatic potential in ovarian cancer and found that fatty acid binding protein (FABP4) can substantially increase the metastasis potential of cancer cells.
References
More filters
Journal ArticleDOI
Integrative analysis of complex cancer genomics and clinical profiles using the cBioPortal
Jianjiong Gao,Bulent Arman Aksoy,Ugur Dogrusoz,Gideon Dresdner,Benjamin Gross,S. Onur Sumer,Yichao Sun,Anders Jacobsen,Rileen Sinha,Erik Larsson,Ethan Cerami,Chris Sander,Nikolaus Schultz +12 more
TL;DR: A practical guide to the analysis and visualization features of the cBioPortal for Cancer Genomics, which makes complex cancer genomics profiles accessible to researchers and clinicians without requiring bioinformatics expertise, thus facilitating biological discoveries.
Journal ArticleDOI
Integrative genomic profiling of human prostate cancer
Barry S. Taylor,Nikolaus Schultz,Haley Hieronymus,Anuradha Gopalan,Yonghong Xiao,Brett S. Carver,Vivek K. Arora,Poorvi Kaushik,Ethan Cerami,Boris Reva,Yevgeniy Antipin,Nicholas Mitsiades,Thomas Landers,Igor Dolgalev,John E. Major,Manda Wilson,Nicholas D. Socci,Alex E. Lash,Adriana Heguy,James A. Eastham,Howard I. Scher,Victor E. Reuter,Peter T. Scardino,Chris Sander,Charles L. Sawyers,Charles L. Sawyers,William L. Gerald +26 more
TL;DR: Analysis of genomic and clinical outcome data from 218 prostate cancer tumors revealed that copy-number alterations robustly define clusters of low- and high-risk disease beyond that achieved by Gleason score.
Journal ArticleDOI
Tumour invasion and metastasis initiated by microRNA-10b in breast cancer
TL;DR: It is shown that miR-10b is highly expressed in metastatic breast cancer cells and positively regulates cell migration and invasion, and the workings of an undescribed regulatory pathway, in which a pleiotropic transcription factor induces expression of a specific microRNA, which suppresses its direct target and in turn activates another pro-metastatic gene, leading to tumour cell invasion and metastasis.
Journal ArticleDOI
Endogenous human microRNAs that suppress breast cancer metastasis
Sohail F. Tavazoie,Claudio Alarcón,Thordur Oskarsson,David Padua,Qiongqing Wang,Paula D. Bos,William L. Gerald,Joan Massagué +7 more
TL;DR: It is shown that restoring the expression of these microRNAs in malignant cells suppresses lung and bone metastasis by human cancer cells in vivo, and miR-126 restoration reduces overall tumour growth and proliferation, whereasmiR-335 inhibits metastatic cell invasion.
Book
Cancer cell lines
TL;DR: 17. Wilms' Tumor and Other Childhood Renal Neoplasms N.Y. Wilson, C.M. Garner, et al.
Related Papers (5)
Exosomal miR-1290 and miR-375 as Prognostic Markers in Castration-resistant Prostate Cancer
Circulating microRNAs as stable blood-based markers for cancer detection
Patrick S. Mitchell,Rachael K. Parkin,Evan M. Kroh,Brian R. Fritz,Brian R. Fritz,Stacia K. Wyman,Era L. Pogosova-Agadjanyan,Amelia Peterson,Jennifer Noteboom,Kathy O'Briant,April Allen,Daniel W. Lin,Daniel W. Lin,Daniel W. Lin,Nicole Urban,Charles W. Drescher,Beatrice S. Knudsen,Derek L. Stirewalt,Robert Gentleman,Robert L. Vessella,Robert L. Vessella,Peter S. Nelson,Daniel Martin,Daniel Martin,Muneesh Tewari +24 more