Modulation of Mucosal Immune Response, Tolerance, and Proliferation in Mice Colonized by the Mucin-Degrader Akkermansia muciniphila
Muriel Derrien,Peter van Baarlen,Guido J. E. J. Hooiveld,Elisabeth Norin,Michael Müller,Willem M. de Vos,Willem M. de Vos +6 more
TLDR
It is proposed that A. muciniphila modulates pathways involved in establishing homeostasis for basal metabolism and immune tolerance toward commensal microbiota, and altered mucosal gene expression profiles toward increased expression of genes involved in immune responses and cell fate determination.Abstract:
Epithelial cells of the mammalian intestine are covered with a mucus layer that prevents direct contact with intestinal microbes but also constitutes a substrate for mucus-degrading bacteria. To study the effect of mucus degradation on the host response, germ-free mice were colonized with Akkermansia muciniphila. This anaerobic bacterium belonging to the Verrucomicrobia is specialized in the degradation of mucin, the glycoprotein present in mucus, and found in high numbers in the intestinal tract of human and other mammalian species. Efficient colonization of A. muciniphila was observed with highest numbers in the cecum, where most mucin is produced. In contrast, following colonization by Lactobacillus plantarum, a facultative anaerobe belonging to the Firmicutes that ferments carbohydrates, similar cell-numbers were found at all intestinal sites. Whereas A. muciniphila was located closely associated with the intestinal cells, L. plantarum was exclusively found in the lumen. The global transcriptional host response was determined in intestinal biopsies and revealed a consistent, site-specific, and unique modulation of about 750 genes in mice colonized by A. muciniphila and over 1500 genes after colonization by L. plantarum. Pathway reconstructions showed that colonization by A. muciniphila altered mucosal gene expression profiles toward increased expression of genes involved in immune responses and cell fate determination, while colonization by L. plantarum led to up-regulation of lipid metabolism. These indicate that the colonizers induce host responses that are specific per intestinal location. In conclusion, we propose that A. muciniphila modulates pathways involved in establishing homeostasis for basal metabolism and immune tolerance toward commensal microbiota.read more
Citations
More filters
Journal ArticleDOI
Supplementation of a propionate-producing consortium improves markers of insulin resistance in an in vitro model of gut-liver axis.
TL;DR: The results suggest that microbiota-derived products can be a promising multipurpose strategy to modulate energy homeostasis, with potential ability to assist in managing metabolic diseases due to their adaptability.
Journal ArticleDOI
Enteric infection coupled with chronic Notch pathway inhibition alters colonic mucus composition leading to dysbiosis, barrier disruption and colitis.
Ishfaq Ahmed,Badal C. Roy,Rita Marie T. Raach,Sarah M. Owens,Lijun Xia,Shrikant Anant,Venkatesh Sampath,Shahid Umar +7 more
TL;DR: Enteric infections that interfere with Notch activity may promote mucosal dysbiosis that is preceded by changes in mucus composition, and controlled use of antibiotics seems to alleviate gut Dysbiosis but may be insufficient to promote colonic crypt regeneration.
Journal ArticleDOI
Human Health and Disease in a Microbial World
Peter J. Turnbaugh,Alain Stintzi +1 more
TL;DR: This collection of reviews, perspectives, and original research articles presents a series of reviews highlighting that the human immune system is intimately linked to the gut microbiome; the gut microbiota influences immune development, susceptibility to infection from pathogens, and inflammation.
Journal ArticleDOI
Mind the PVCs.
Damien P. Devos,Naomi L. Ward +1 more
TL;DR: Most Chlamydiae are pathogens commonly found in mammals and multicellular eukaryotes, although many new lineages are associated with marine water and free-living amoebae.
Journal ArticleDOI
Potential anti-obesogenic properties of non-digestible carbohydrates: specific focus on resistant dextrin
TL;DR: Evidence from mechanistic and intervention studies is provided that certain fermentable NDC, including resistant dextrin, are able to modulate the gut microbiota and may alter metabolic process associated with obesity, including appetite regulation, energy and lipid metabolism and inflammation.
References
More filters
疟原虫var基因转换速率变化导致抗原变异[英]/Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
An obesity-associated gut microbiome with increased capacity for energy harvest
Peter J. Turnbaugh,Ruth E. Ley,Michael A. Mahowald,Vincent Magrini,Elaine R. Mardis,Jeffrey I. Gordon +5 more
TL;DR: It is demonstrated through metagenomic and biochemical analyses that changes in the relative abundance of the Bacteroidetes and Firmicutes affect the metabolic potential of the mouse gut microbiota and indicates that the obese microbiome has an increased capacity to harvest energy from the diet.
Journal ArticleDOI
Diversity of the human intestinal microbial flora.
Paul B. Eckburg,Elisabeth M. Bik,Charles N. Bernstein,Elizabeth Purdom,Les Dethlefsen,Michael Sargent,Steven R. Gill,Karen E. Nelson,David A. Relman,David A. Relman,David A. Relman +10 more
TL;DR: A majority of the bacterial sequences corresponded to uncultivated species and novel microorganisms, and significant intersubject variability and differences between stool and mucosa community composition were discovered.
Journal ArticleDOI
Obesity alters gut microbial ecology
Ruth E. Ley,Fredrik Bäckhed,Peter J. Turnbaugh,Catherine A. Lozupone,Robin D. Knight,Jeffrey I. Gordon +5 more
TL;DR: Analysis of the microbiota of genetically obese ob/ob mice, lean ob/+ and wild-type siblings, and their ob/+ mothers, all fed the same polysaccharide-rich diet, indicates that obesity affects the diversity of the gut microbiota and suggests that intentional manipulation of community structure may be useful for regulating energy balance in obese individuals.
Journal ArticleDOI
The gut microbiota as an environmental factor that regulates fat storage
Fredrik Bäckhed,Hao Ding,Hao Ding,Ting Wang,Lora V. Hooper,Gou Young Koh,Andras Nagy,Clay F. Semenkovich,Jeffrey I. Gordon +8 more
TL;DR: In this article, the authors found that conventionalization of adult germ-free C57BL/6 mice with a normal microbiota harvested from the distal intestine (cecum) of conventionally raised animals produces a 60% increase in body fat content and insulin resistance within 14 days despite reduced food intake.
Related Papers (5)
QIIME allows analysis of high-throughput community sequencing data.
J. Gregory Caporaso,Justin Kuczynski,Jesse Stombaugh,Kyle Bittinger,Frederic D. Bushman,Elizabeth K. Costello,Noah Fierer,Antonio Gonzalez Peña,Julia K. Goodrich,Jeffrey I. Gordon,Gavin A. Huttley,Scott T. Kelley,Dan Knights,Jeremy E. Koenig,Ruth E. Ley,Catherine A. Lozupone,Daniel McDonald,Brian D. Muegge,Meg Pirrung,Jens Reeder,Joel Sevinsky,Peter J. Turnbaugh,William A. Walters,Jeremy Widmann,Tanya Yatsunenko,Jesse R. Zaneveld,Rob Knight,Rob Knight +27 more
A purified membrane protein from Akkermansia muciniphila or the pasteurized bacterium improves metabolism in obese and diabetic mice
Hubert Plovier,Amandine Everard,Céline Druart,Clara Depommier,Matthias Van Hul,Lucie Geurts,Julien Chilloux,Noora Ottman,Thibaut Duparc,Laeticia Lichtenstein,Antonis Myridakis,Nathalie M. Delzenne,Judith Klievink,Arnab Bhattacharjee,Kees C. H. van der Ark,Steven Aalvink,Laurent O. Martinez,Marc-Emmanuel Dumas,Dominique Maiter,Audrey Loumaye,Michel P. Hermans,Jean-Paul Thissen,Clara Belzer,Willem M. de Vos,Willem M. de Vos,Patrice D. Cani +25 more