Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
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Journal ArticleDOI
Direct-current output of silicon–organic monolayer–platinum Schottky TENGs: Elusive friction-output relationship
TL;DR: In this paper , the authors used dynamic and static conductive atomic force microscopy (C-AFM) measurements on monolayer-modified silicon crystals to detect evidence of a relationship between friction and zero-bias current, and between pressure and the direction of the putative flexovoltage.
Book ChapterDOI
Imaging and Force Spectroscopy of Single Transmembrane Proteins with the Atomic Force Microscope.
TL;DR: Practical and analytical protocols of single-molecule AFM methodologies related to high-resolution imaging and single- molecule force spectroscopy of transmembrane proteins in a lipid bilayer (reconstituted or native).
Book ChapterDOI
Imaging Membranes by High-Resolution Atomic Force Microscopy
Mingjun Cai,Jing Gao,Hongda Wang +2 more
TL;DR: High-resolution imaging of morphological details demonstrated that the membrane proteins are mainly located on the cytoplasmic side of membranes, and the ectoplasmicSide membrane is smooth.
Journal ArticleDOI
Imaging the subcellular viscoelastic properties of mouse oocytes.
Guillaume Fle,Elijah E. W. Van Houten,Gaudeline Rémillard-Labrosse,Greg FitzHarris,Guy Cloutier +4 more
TL;DR: In this article , a framework for mapping viscoelasticity at the subcellular scale is proposed and applied to live mouse oocytes, which relies on the principles of optical microelastography for imaging in combination with the overlapping subzone nonlinear inversion technique for complex-valued shear modulus reconstruction.
Book ChapterDOI
High-resolution imaging and force spectroscopy of single membrane proteins by atomic force microscopy
TL;DR: In this article , atomic force microscopy (AFMEM) was used to image the topographical structures and detect the mechanical properties of single membrane proteins in their native states, considerably complementing the studies of membrane proteins by traditional methods and remarkably providing novel insights into the biophysics of membrane protein with molecular resolution.
References
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Journal ArticleDOI
Models for the specific adhesion of cells to cells
TL;DR: The force required to separate two cells is shown to be greater than the expected electrical forces between cells, and of the same order of magnitude as the forces required to pull gangliosides and perhaps some integral membrane proteins out of the cell membrane.
Journal ArticleDOI
Force measurements with the atomic force microscope: Technique, interpretation and applications
TL;DR: The atomic force microscope (AFM) is not only used to image the topography of solid surfaces at high resolution but also to measure force-versus-distance curves as discussed by the authors, which provide valuable information on local material properties such as elasticity, hardness, Hamaker constant, adhesion and surface charge densities.
Journal ArticleDOI
Single-molecule mechanics of mussel adhesion
TL;DR: A single-molecule study of the substrate and oxidation-dependent adhesive properties of dopa is reported, in which dopa exploits a remarkable combination of high strength and chemical multifunctionality to accomplish adhesion to substrates of widely varying composition.
Journal ArticleDOI
Nanomechanical analysis of cells from cancer patients
TL;DR: This work reports the stiffness of live metastatic cancer cells taken from the body fluids of patients with suspected lung, breast and pancreas cancer, and shows that nanomechanical analysis correlates well with immunohistochemical testing currently used for detecting cancer.
Journal ArticleDOI
Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.