Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
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Journal ArticleDOI
GSDMD membrane pore formation constitutes the mechanism of pyroptotic cell death
Lorenzo Sborgi,Sebastian Rühl,Estefania Mulvihill,Joka Pipercevic,Rosalie Heilig,Henning Stahlberg,Christopher J. Farady,Daniel J. Müller,Petr Broz,Sebastian Hiller +9 more
TL;DR: Overall, these data demonstrate that GSDMD is the direct and final executor of pyroptotic cell death.
Journal ArticleDOI
Imaging modes of atomic force microscopy for application in molecular and cell biology
Yves F. Dufrêne,Toshio Ando,Ricardo Garcia,David Alsteens,David Martinez-Martin,Andreas Engel,Christoph Gerber,Daniel J. Müller +7 more
TL;DR: The basic principles, advantages and limitations of the most common AFM bioimaging modes are reviewed, including the popular contact and dynamic modes, as well as recently developed modes such as multiparametric, molecular recognition, multifrequency and high-speed imaging.
Journal ArticleDOI
Tau protein liquid–liquid phase separation can initiate tau aggregation
Susanne Wegmann,Bahareh Eftekharzadeh,Katharina Tepper,Katarzyna Marta Zoltowska,Rachel E. Bennett,Simon Dujardin,Pawel R. Laskowski,Danny MacKenzie,Tarun V. Kamath,Caitlin Commins,Charles R. Vanderburg,Allyson D. Roe,Zhanyun Fan,Amandine Molliex,Amayra Hernández-Vega,Daniel J. Müller,Anthony A. Hyman,Eckhard Mandelkow,Eckhard Mandelkow,J. Paul Taylor,J. Paul Taylor,Bradley T. Hyman +21 more
TL;DR: It is demonstrated that phosphorylated or mutant aggregation prone recombinant tau undergoes LLPS, as does high molecular weight soluble phospho‐tau isolated from human Alzheimer brain, and it is suggested that LLPS represents a biophysical process with a role in multiple different neurodegenerative diseases.
Journal ArticleDOI
Atomic force microscopy-based mechanobiology
Michael Krieg,Gotthold Fläschner,David Alsteens,Benjamin M. Gaub,Wouter H. Roos,Gijs J.L. Wuite,Hermann E. Gaub,Christoph Gerber,Yves F. Dufrêne,Daniel J. Müller +9 more
TL;DR: The potential of combining AFM with complementary techniques, including optical microscopy and spectroscopy of mechanosensitive fluorescent constructs, super-resolution microscopy, the patch clamp technique and the use of microstructured and fluidic devices to characterize the 3D distribution of mechanical responses within biological systems and to track their morphology and functional state as discussed by the authors.
Journal ArticleDOI
Bacterial adhesion at the single-cell level
TL;DR: Understanding of the mechanisms governing bacterial adhesion at the single-cell level is summarized, including the physical forces experienced by a cell before reaching the surface, the first contact with a surface and the transition from reversible to permanent adhesion.
References
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Journal ArticleDOI
Atomic Force Microscopy Measurement of Heterogeneity in Bacterial Surface Hydrophobicity
TL;DR: The use of atomic force microscopy (AFM) is reported to explore the morphology of soft, living cells in aqueous buffer, to map bacterial surface heterogeneities, and to directly correlate the results in the AFM force-distance curves to the macroscopic properties of the microbial surfaces.
Journal ArticleDOI
Quantification of cell adhesion force with AFM: distribution of vitronectin receptors on a living MC3T3-E1 cell.
TL;DR: Distribution of vitronectin (VN) receptors on a living murine osteoblastic cell was successfully measured by atomic force microscopy by using a microbead attached to the cantilever tip to increase the area of contact and VN was immobilized on the microBead.
Journal ArticleDOI
A nanomechanical interface to rapid single-molecule interactions
Mingdong Dong,Ozgur Sahin +1 more
TL;DR: A nanomechanical sensor is reported that allows single-molecule force spectroscopy on the previously unexplored microsecond timescale and provides experimental evidence for an additional energy barrier in the biotin-streptavidin complex.
Journal ArticleDOI
Recognition imaging and highly ordered molecular templating of bacterial S-layer nanoarrays containing affinity-tags.
Jilin Tang,Andreas Ebner,Helga Badelt-Lichtblau,Christine Völlenkle,Christian Rankl,Bernhard Kraxberger,Michael Leitner,Linda Wildling,Hermann J. Gruber,Uwe B. Sleytr,Nicola Ilk,Peter Hinterdorfer +11 more
TL;DR: Functional nanoarrays were fabricated using the chimeric bacterial cell surface layer (S-layer) protein rSbpA fused with the affinity tag Strep-tagII and characterized using various atomic force microscopy techniques in aqueous environment to construct highly ordered molecular binding blocks.
Journal ArticleDOI
Built-In Mechanical Stress in Viral Shells
Carolina Carrasco,Antoni Luque,Mercedes Hernando-Pérez,Roberto Miranda,José L. Carrascosa,Pedro A. Serena,M. de Ridder,Arvind Raman,Julio Gómez-Herrero,Iwan A. T. Schaap,David Reguera,P. J. de Pablo +11 more
TL;DR: It is shown that empty prolated φ29 bacteriophage proheads exhibit an intriguing anisotropic stiffness which behaves counterintuitively different from standard continuum elasticity predictions.