Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
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Journal ArticleDOI
GSDMD membrane pore formation constitutes the mechanism of pyroptotic cell death
Lorenzo Sborgi,Sebastian Rühl,Estefania Mulvihill,Joka Pipercevic,Rosalie Heilig,Henning Stahlberg,Christopher J. Farady,Daniel J. Müller,Petr Broz,Sebastian Hiller +9 more
TL;DR: Overall, these data demonstrate that GSDMD is the direct and final executor of pyroptotic cell death.
Journal ArticleDOI
Imaging modes of atomic force microscopy for application in molecular and cell biology
Yves F. Dufrêne,Toshio Ando,Ricardo Garcia,David Alsteens,David Martinez-Martin,Andreas Engel,Christoph Gerber,Daniel J. Müller +7 more
TL;DR: The basic principles, advantages and limitations of the most common AFM bioimaging modes are reviewed, including the popular contact and dynamic modes, as well as recently developed modes such as multiparametric, molecular recognition, multifrequency and high-speed imaging.
Journal ArticleDOI
Tau protein liquid–liquid phase separation can initiate tau aggregation
Susanne Wegmann,Bahareh Eftekharzadeh,Katharina Tepper,Katarzyna Marta Zoltowska,Rachel E. Bennett,Simon Dujardin,Pawel R. Laskowski,Danny MacKenzie,Tarun V. Kamath,Caitlin Commins,Charles R. Vanderburg,Allyson D. Roe,Zhanyun Fan,Amandine Molliex,Amayra Hernández-Vega,Daniel J. Müller,Anthony A. Hyman,Eckhard Mandelkow,Eckhard Mandelkow,J. Paul Taylor,J. Paul Taylor,Bradley T. Hyman +21 more
TL;DR: It is demonstrated that phosphorylated or mutant aggregation prone recombinant tau undergoes LLPS, as does high molecular weight soluble phospho‐tau isolated from human Alzheimer brain, and it is suggested that LLPS represents a biophysical process with a role in multiple different neurodegenerative diseases.
Journal ArticleDOI
Atomic force microscopy-based mechanobiology
Michael Krieg,Gotthold Fläschner,David Alsteens,Benjamin M. Gaub,Wouter H. Roos,Gijs J.L. Wuite,Hermann E. Gaub,Christoph Gerber,Yves F. Dufrêne,Daniel J. Müller +9 more
TL;DR: The potential of combining AFM with complementary techniques, including optical microscopy and spectroscopy of mechanosensitive fluorescent constructs, super-resolution microscopy, the patch clamp technique and the use of microstructured and fluidic devices to characterize the 3D distribution of mechanical responses within biological systems and to track their morphology and functional state as discussed by the authors.
Journal ArticleDOI
Bacterial adhesion at the single-cell level
TL;DR: Understanding of the mechanisms governing bacterial adhesion at the single-cell level is summarized, including the physical forces experienced by a cell before reaching the surface, the first contact with a surface and the transition from reversible to permanent adhesion.
References
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Journal ArticleDOI
Observing single biomolecules at work with the atomic force microscope.
Andreas Engel,Daniel J. Müller +1 more
TL;DR: The unique capability of the AFM to directly observe single proteins in their native environments provides insights into the interactions of proteins that form functional assemblies and provides unprecedented possibilities for analyzing intramolecular and intermolecular forces.
Journal ArticleDOI
The emergence of multifrequency force microscopy
Ricardo Garcia,Elena T. Herruzo +1 more
TL;DR: Five different modes of multifrequency force microscopy are reviewed and their application in studies of proteins, the imaging of vibrating nanostructures, measurements of ion diffusion and subsurface imaging in cells is examined.
Journal ArticleDOI
Forces and Bond Dynamics in Cell Adhesion
Evan Evans,David A. Calderwood +1 more
TL;DR: Combining molecular cell biology with single-molecule force spectroscopy provides a powerful tool for exploring the complexity of cell adhesion, that is, how cell signaling processes strengthen adhesion bonds and how forces applied to cell-surface bonds act on intracellular sites to catalyze chemical processes or switch molecular interactions on and off.
Journal ArticleDOI
Sensing specific molecular interactions with the atomic force microscope
Ernst-Ludwig Florin,Matthias Rief,Horst Lehmann,Markus Ludwig,C. Dornmair,Vincent T. Moy,Hermann E. Gaub +6 more
TL;DR: Here, different techniques that allow for the in situ measurement of the absolute value of the spring constant of the cantilevers are compared.
Journal ArticleDOI
Specific antigen/antibody interactions measured by force microscopy
U. Dammer,Martin Hegner,Dario Anselmetti,Peter Wagner,M Dreier,Walter Huber,Hans-Joachim Güntherodt +6 more
TL;DR: It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to their antigens when probed with an AFM in the force mode, and the rupture force between individual antibody-antigen complexes was measured.