Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
More filters
Journal ArticleDOI
Probing of Nanoscale Friction and Mechanical Characteristics of Cotton Fiber’s Surface
TL;DR: In this article, the surface topography and nanomechanical attributes of two samples of cotton fibers, namely, A and B, were characterized with various operation modes of an Atomic Force Microscope (AFM).
Journal ArticleDOI
Les calpaïnes - Une arme à double tranchant
TL;DR: In this paper, the role of mediateurs anti-inflammatoires, proteiques, and lipidiques is discussed, and a switch in the metabolism of l’acide arachidonique is described.
Book
Atomic Force Microscopy: A New Look at Microbes
TL;DR: In this article, the authors highlight the wealth of AFM-based modalities that have been implemented over the recent years leading to the multiparametric and multifunctional characterization of, specifically, bacterial surfaces.
Journal ArticleDOI
From fixed-dried to wet-fixed to live – comparative super-resolution microscopy of liver sinusoidal endothelial cell fenestrations
Karolina Szafranska,Tanja Neuman,Zbigniew Baster,Zenon Rajfur,Oskar Szelest,Christopher Florian Holte,Agata Kubisiak,Edyta Kus,Deanna Wolfson,Stefan Chlopicki,Balpreet Singh Ahluwalia,Małgorzata Lekka,Marek Szymonski,Peter McCourt,Bartlomiej Zapotoczny +14 more
TL;DR: It is concluded that sample dehydration alters fenestration diameters, and the combination of AFM with conventional microscopy is proposed that allows for easy super-resolution observation of the cell dynamics with additional chemical information that can be traced back for the whole experiment.
Journal ArticleDOI
Local permittivity measurement of dielectric materials based on the non-contact force curve of microwave atomic force microscopy
TL;DR: A non-contact and quantitative method to measure the local permittivity of dielectric materials with a nanometer-scale spatial resolution and observed the variation in the force caused by the microwave.
References
More filters
Journal ArticleDOI
Models for the specific adhesion of cells to cells
TL;DR: The force required to separate two cells is shown to be greater than the expected electrical forces between cells, and of the same order of magnitude as the forces required to pull gangliosides and perhaps some integral membrane proteins out of the cell membrane.
Journal ArticleDOI
Force measurements with the atomic force microscope: Technique, interpretation and applications
TL;DR: The atomic force microscope (AFM) is not only used to image the topography of solid surfaces at high resolution but also to measure force-versus-distance curves as discussed by the authors, which provide valuable information on local material properties such as elasticity, hardness, Hamaker constant, adhesion and surface charge densities.
Journal ArticleDOI
Single-molecule mechanics of mussel adhesion
TL;DR: A single-molecule study of the substrate and oxidation-dependent adhesive properties of dopa is reported, in which dopa exploits a remarkable combination of high strength and chemical multifunctionality to accomplish adhesion to substrates of widely varying composition.
Journal ArticleDOI
Nanomechanical analysis of cells from cancer patients
TL;DR: This work reports the stiffness of live metastatic cancer cells taken from the body fluids of patients with suspected lung, breast and pancreas cancer, and shows that nanomechanical analysis correlates well with immunohistochemical testing currently used for detecting cancer.
Journal ArticleDOI
Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.