Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
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Journal ArticleDOI
Generation of living cell arrays for atomic force microscopy studies
Cécile Formosa,Flavien Pillet,Flavien Pillet,Marion Schiavone,Raphaël E. Duval,Raphaël E. Duval,Laurence Ressier,Laurence Ressier,Etienne Dague,Etienne Dague +9 more
TL;DR: This protocol describes how to immobilize living cells, ranging from spores of bacteria to yeast cells, into polydimethylsiloxane (PDMS) stamps, with no chemical or physical denaturation.
Journal ArticleDOI
Fundamental High-Speed Limits in Single-Molecule, Single-Cell, and Nanoscale Force Spectroscopies
Carlos A. Amo,Ricardo Garcia +1 more
TL;DR: This work demonstrates that the total force acting on the probe has three different components: the interaction, the hydrodynamic, and the inertial, which depend on the ratio between the resonant frequency and the frequency at which the data are measured.
Journal ArticleDOI
Combining confocal and atomic force microscopy to quantify single-virus binding to mammalian cell surfaces
Richard Newton,Martin Delguste,Melanie Koehler,Andra C. Dumitru,Pawel R. Laskowski,Daniel J. Müller,David Alsteens +6 more
TL;DR: Detailed procedures for probing the specific interactions of viruses with living cells are described; these procedures cover tip preparation, cell sample preparation, step-by-step FD-based AFM imaging and data analysis, and should be able to master the entire set of protocols in 1 month.
Journal ArticleDOI
Automated AFM force curve analysis for determining elastic modulus of biomaterials and biological samples
Yow-Ren Chang,Vijay Krishna Raghunathan,Shaun P. Garland,Joshua T. Morgan,Paul Russell,Christopher J. Murphy +5 more
TL;DR: An automated algorithm for the selection of CPs in AFM force data and the evaluation of elastic moduli is presented and it is proposed that CP may be algorithmically easier to detect by identifying a linear elastic indentation region of data (high SNR) rather than the contact point itself (low SNR).
Journal ArticleDOI
Multiparametric AFM reveals turgor-responsive net-like peptidoglycan architecture in live streptococci.
TL;DR: This work shows a new net-like arrangement of PG, which stretches and stiffens following osmotic challenge in live Group B Streptococcus, and the same structure also exists in isogenic mutants lacking surface appendages.
References
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Journal ArticleDOI
Models for the specific adhesion of cells to cells
TL;DR: The force required to separate two cells is shown to be greater than the expected electrical forces between cells, and of the same order of magnitude as the forces required to pull gangliosides and perhaps some integral membrane proteins out of the cell membrane.
Journal ArticleDOI
Force measurements with the atomic force microscope: Technique, interpretation and applications
TL;DR: The atomic force microscope (AFM) is not only used to image the topography of solid surfaces at high resolution but also to measure force-versus-distance curves as discussed by the authors, which provide valuable information on local material properties such as elasticity, hardness, Hamaker constant, adhesion and surface charge densities.
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Single-molecule mechanics of mussel adhesion
TL;DR: A single-molecule study of the substrate and oxidation-dependent adhesive properties of dopa is reported, in which dopa exploits a remarkable combination of high strength and chemical multifunctionality to accomplish adhesion to substrates of widely varying composition.
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Nanomechanical analysis of cells from cancer patients
TL;DR: This work reports the stiffness of live metastatic cancer cells taken from the body fluids of patients with suspected lung, breast and pancreas cancer, and shows that nanomechanical analysis correlates well with immunohistochemical testing currently used for detecting cancer.
Journal ArticleDOI
Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.