Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
More filters
Journal ArticleDOI
The microbial adhesive arsenal deciphered by atomic force microscopy
TL;DR: Some of the major breakthroughs facilitated by AFM in deciphering the microbial adhesive arsenal are covered, including the exciting development of anti-adhesive strategies.
Journal ArticleDOI
Accurate Wide-Modulus-Range Nanomechanical Mapping of Ultrathin Interfaces with Bimodal Atomic Force Microscopy.
Victor G. Gisbert,Ricardo Garcia +1 more
TL;DR: In this article, a bottom-effect correction method that yields the intrinsic Young's modulus value of a material independent of its thickness is proposed. But the method is limited to the case of thin materials and interfaces.
Journal ArticleDOI
Viability of Cladosporium herbarum spores under 157 nm laser and vacuum ultraviolet irradiation, low temperature (10 K) and vacuum
Evangelia Sarantopoulou,Aikaterina L. Stefi,Zoe Kollia,Dimitrios Palles,P. S. Petrou,Athanasia Bourkoula,G. Koukouvinos,Athanassios D. Velentzas,S.E. Kakabakos,A.C. Cefalas +9 more
TL;DR: In this paper, the authors showed that Cladosporium herbarum spore monolayers exhibit high viability (7%) when exposed to 157 nm laser irradiation (412 kJm−2) or vacuum-ultraviolet irradiation under standard pressure and temperature in a nitrogen atmosphere.
Journal ArticleDOI
Physiological, vascular and nanomechanical assessment of hybrid poplar leaf traits in micropropagated plants and plants propagated from root cuttings: A contribution to breeding programs.
Jaroslav Ďurkovič,Hana Husárová,Lucia Javoříková,Ingrid Čaňová,Miriama Šuleková,Monika Kardošová,Ivan Lukáčik,Miroslava Mamoňová,Rastislav Lagaňa +8 more
TL;DR: The performance of the plants propagated from root cuttings was superior for instantaneous water-use efficiency which signifies a higher acclimation capacity to stressful conditions during a severe drought particularly for this stock type.
Book ChapterDOI
Pathogenesis of biomaterial-associated infection
TL;DR: Recognition of the importance of the immune system interaction in the pathogenesis of biomaterial-associated infections will not only update the paradigm of this condition but also help to identify and develop potential therapeutic targets.
References
More filters
Journal ArticleDOI
Models for the specific adhesion of cells to cells
TL;DR: The force required to separate two cells is shown to be greater than the expected electrical forces between cells, and of the same order of magnitude as the forces required to pull gangliosides and perhaps some integral membrane proteins out of the cell membrane.
Journal ArticleDOI
Force measurements with the atomic force microscope: Technique, interpretation and applications
TL;DR: The atomic force microscope (AFM) is not only used to image the topography of solid surfaces at high resolution but also to measure force-versus-distance curves as discussed by the authors, which provide valuable information on local material properties such as elasticity, hardness, Hamaker constant, adhesion and surface charge densities.
Journal ArticleDOI
Single-molecule mechanics of mussel adhesion
TL;DR: A single-molecule study of the substrate and oxidation-dependent adhesive properties of dopa is reported, in which dopa exploits a remarkable combination of high strength and chemical multifunctionality to accomplish adhesion to substrates of widely varying composition.
Journal ArticleDOI
Nanomechanical analysis of cells from cancer patients
TL;DR: This work reports the stiffness of live metastatic cancer cells taken from the body fluids of patients with suspected lung, breast and pancreas cancer, and shows that nanomechanical analysis correlates well with immunohistochemical testing currently used for detecting cancer.
Journal ArticleDOI
Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.