Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
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Surface imaging beyond the diffraction limit with optically trapped spheres
TL;DR: It is demonstrated that, by the combination of a time-shared twin-optical trap and nanometre-precise three-dimensional interferometric particle tracking, both reliable height profiling and surface imaging are possible with a spatial resolution below the diffraction limit.
Journal ArticleDOI
Unraveling the Pore-Forming Steps of Pneumolysin from Streptococcus pneumoniae.
TL;DR: A four-step mechanism of membrane attachment and pore formation by PLY is suggested, which is discussed in the context of recent structural models for the understanding how cholesterol-dependent cytolysins form pores and lyse cells.
Journal ArticleDOI
Multivalent binding of herpesvirus to living cells is tightly regulated during infection
TL;DR: Biophysical results show that the major envelope glycoprotein functions as a regulator of binding valency during both attachment and release steps, determining the binding, diffusion, and release potential of virions at the cellular surface.
Journal ArticleDOI
Nanoscale membrane architecture of healthy and pathological red blood cells.
Andra-Cristina Dumitru,Mégane A. Poncin,Louise Conrard,Yves F. Dufrêne,Donatienne Tyteca,David Alsteens +5 more
TL;DR: Atomic force microscopy multiparametric imaging is used to probe how cellular organization influences nanoscale and global mechanical properties of cells in both physiological and pathological conditions and finds that red blood cells from patients with hereditary spherocytosis are stiffer.
Journal ArticleDOI
Progress in the Correlative Atomic Force Microscopy and Optical Microscopy
TL;DR: In this review, the principles of AFM and optical microscopy, such as confocal microscopy and single-molecule localization microscopy are reported, and the development and use of correlative AFm and optical microscopeopy are focused on.
References
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Journal ArticleDOI
Models for the specific adhesion of cells to cells
TL;DR: The force required to separate two cells is shown to be greater than the expected electrical forces between cells, and of the same order of magnitude as the forces required to pull gangliosides and perhaps some integral membrane proteins out of the cell membrane.
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Force measurements with the atomic force microscope: Technique, interpretation and applications
TL;DR: The atomic force microscope (AFM) is not only used to image the topography of solid surfaces at high resolution but also to measure force-versus-distance curves as discussed by the authors, which provide valuable information on local material properties such as elasticity, hardness, Hamaker constant, adhesion and surface charge densities.
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Single-molecule mechanics of mussel adhesion
TL;DR: A single-molecule study of the substrate and oxidation-dependent adhesive properties of dopa is reported, in which dopa exploits a remarkable combination of high strength and chemical multifunctionality to accomplish adhesion to substrates of widely varying composition.
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Nanomechanical analysis of cells from cancer patients
TL;DR: This work reports the stiffness of live metastatic cancer cells taken from the body fluids of patients with suspected lung, breast and pancreas cancer, and shows that nanomechanical analysis correlates well with immunohistochemical testing currently used for detecting cancer.
Journal ArticleDOI
Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.