Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
More filters
Journal ArticleDOI
Single cell profiling of surface carbohydrates on Bacillus cereus
TL;DR: AFM-based ‘recognition force mapping’ is demonstrated as a versatile platform to quantitatively detect and spatially map key bacterial surface biomarkers (such as carbohydrate compositions), and monitor in situ changes in surface biochemical properties during intracellular activities at the single cell level.
Journal ArticleDOI
Overlapping and essential roles for molecular and mechanical mechanisms in mycobacterial cell division
Pascal D. Odermatt,Mélanie T. M. Hannebelle,Haig A. Eskandarian,Adrian P. Nievergelt,John D. McKinney,Georg E. Fantner +5 more
TL;DR: Atomic force microscopy reveals that the accumulation of mechanical stress works together with enzymatic activity to ensure the rapid cleavage of dividing bacteria.
Journal ArticleDOI
Atomic force microscopy of membrane pore formation by cholesterol dependent cytolysins.
TL;DR: Atomic force microscopy provides real-time perspective on membrane pore formation and has been successfully applied to cholesterol-dependent cytolysins (CDCs) and seen to transform from mobile prepore assemblies into transmembrane pores.
Journal ArticleDOI
Scratching the Surface: Bacterial Cell Envelopes at the Nanoscale.
TL;DR: Recent progress in the use of AFM imaging for understanding the architecture and dynamics of the bacterial envelope is discussed, and the power of the technique to observe isolated membranes and live cells at (sub)nanometer resolution and under physiological conditions and to track in vitro structural dynamics in response to growth or to drugs is surveyed.
Journal ArticleDOI
Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy
TL;DR: Based on the authors’ own researches on AFM measurement of cellular mechanical properties, the principle and method of AFM indentation technique was presented, the recent progress of measuring the mechanical properties using AFM was summarized, and the challenges ofAFM single-cell nanomechanical analysis were discussed.
References
More filters
Journal ArticleDOI
Models for the specific adhesion of cells to cells
TL;DR: The force required to separate two cells is shown to be greater than the expected electrical forces between cells, and of the same order of magnitude as the forces required to pull gangliosides and perhaps some integral membrane proteins out of the cell membrane.
Journal ArticleDOI
Force measurements with the atomic force microscope: Technique, interpretation and applications
TL;DR: The atomic force microscope (AFM) is not only used to image the topography of solid surfaces at high resolution but also to measure force-versus-distance curves as discussed by the authors, which provide valuable information on local material properties such as elasticity, hardness, Hamaker constant, adhesion and surface charge densities.
Journal ArticleDOI
Single-molecule mechanics of mussel adhesion
TL;DR: A single-molecule study of the substrate and oxidation-dependent adhesive properties of dopa is reported, in which dopa exploits a remarkable combination of high strength and chemical multifunctionality to accomplish adhesion to substrates of widely varying composition.
Journal ArticleDOI
Nanomechanical analysis of cells from cancer patients
TL;DR: This work reports the stiffness of live metastatic cancer cells taken from the body fluids of patients with suspected lung, breast and pancreas cancer, and shows that nanomechanical analysis correlates well with immunohistochemical testing currently used for detecting cancer.
Journal ArticleDOI
Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.