Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
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Design and use of model membranes to study biomolecular interactions using complementary surface-sensitive techniques.
Luke A. Clifton,Richard A. Campbell,Federica Sebastiani,José Campos-Terán,Juan F. Gonzalez-Martinez,Sebastian Björklund,Javier Sotres,Marité Cárdenas +7 more
TL;DR: This review will present the main planar model membranes used in the field to date with a focus on monolayer at the air-liquid interface, supported lipid bilayers at the solid- liquid interface and advanced membrane models such as tethered and floating membranes.
Journal ArticleDOI
Nanomechanical Mapping of Hydrated Rat Tail Tendon Collagen I Fibrils
TL;DR: It is proposed that collagen I fibrils may be in a glassy state while hydrated as well as a similar behavior observed for cross-linked polymer networks below the glass transition temperature.
Journal ArticleDOI
High-Speed AFM Images of Thermal Motion Provide Stiffness Map of Interfacial Membrane Protein Moieties
Johannes Preiner,Andreas Horner,Andreas Karner,Nicole Ollinger,Christine Siligan,Peter Pohl,Peter Hinterdorfer +6 more
TL;DR: Stiffness maps of the main periplasmic loops of single reconstituted water channels revealed the spatial and temporal organization of loop-stabilizing intraproteinaceous H-bonds and salt bridges.
Journal ArticleDOI
Atomic force microscopy: From red blood cells to immunohaematology
TL;DR: The applications of AFM in determining RBC health by the observation of cell morphology, elasticity and surface roughness are described and the reaction of RBC to changes in the physico-chemistry of its environment and the presence of R BC surface antigen specific-biomolecules is achievable.
Journal ArticleDOI
Solid surface vs. liquid surface: nanoarchitectonics, molecular machines, and DNA origami.
TL;DR: Recent research examples of nanoarchitectonics, molecular machines, DNA nanotechnology, and DNA origami are compared with respect to the type of surface used, i.e. solid surfaces vs. liquid surfaces, for future perspectives of interfacial physics and chemistry.
References
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Journal ArticleDOI
Models for the specific adhesion of cells to cells
TL;DR: The force required to separate two cells is shown to be greater than the expected electrical forces between cells, and of the same order of magnitude as the forces required to pull gangliosides and perhaps some integral membrane proteins out of the cell membrane.
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Force measurements with the atomic force microscope: Technique, interpretation and applications
TL;DR: The atomic force microscope (AFM) is not only used to image the topography of solid surfaces at high resolution but also to measure force-versus-distance curves as discussed by the authors, which provide valuable information on local material properties such as elasticity, hardness, Hamaker constant, adhesion and surface charge densities.
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Single-molecule mechanics of mussel adhesion
TL;DR: A single-molecule study of the substrate and oxidation-dependent adhesive properties of dopa is reported, in which dopa exploits a remarkable combination of high strength and chemical multifunctionality to accomplish adhesion to substrates of widely varying composition.
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Nanomechanical analysis of cells from cancer patients
TL;DR: This work reports the stiffness of live metastatic cancer cells taken from the body fluids of patients with suspected lung, breast and pancreas cancer, and shows that nanomechanical analysis correlates well with immunohistochemical testing currently used for detecting cancer.
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Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.